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Cell cryopreservation solution and cryopreservation method

A cryopreservation method and cryopreservation liquid technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of reducing the effect of cell cryopreservation and low cell survival rate, and achieve cell survival rate guarantee, The effect of simple freezing method and low operation difficulty

Inactive Publication Date: 2020-10-16
西部医学科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a cell cryopreservation solution and a cryopreservation method to solve the problem that the existing cell cryopreservation method proposed in the above-mentioned background technology leads to a low cell survival rate, thereby reducing the effect of cell cryopreservation

Method used

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  • Cell cryopreservation solution and cryopreservation method
  • Cell cryopreservation solution and cryopreservation method
  • Cell cryopreservation solution and cryopreservation method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The specific steps of the cryopreservation method of the cell cryopreservation solution are as follows:

[0037] S1: Pre-cooling: The composition of the cell cryopreservation solution contains 22 mg of fetal bovine serum, 12 mg of dimethyl sulfoxide, and 66 mg of 1640 culture medium per 100 mg according to the weight ratio. Replace the cryopreservation medium with a new cell cryopreservation medium, select exponentially growing cells for centrifugation, obtain the centrifuged cells and place them in the cell cryopreservation medium, the cell content in the cryopreservation tube is 1-3*10 6 cells / ml, store the cell cryopreservation solution containing the centrifuged cells in a cryopreservation tube, and place the cryopreservation tube at a temperature of 3-4°C for 10-15 minutes for pre-cooling;

[0038] S2: Preliminary freezing: Take out the cryopreservation tube pre-cooled in step S1 and store it at -20°C for 30-40 minutes for preliminary freezing;

[0039] S3: Second...

Embodiment 2

[0041] The specific steps of the cryopreservation method of the cell cryopreservation solution are as follows:

[0042] S1: Pre-cooling: The composition of the cell cryopreservation solution contains 20 mg of fetal bovine serum, 10 mg of dimethyl sulfoxide, and 70 mg of 1640 culture medium per 100 mg according to the weight ratio. Replace the cryopreservation medium with a new cell cryopreservation medium, select exponentially growing cells for centrifugation, obtain the centrifuged cells and place them in the cell cryopreservation medium, the cell content in the cryopreservation tube is 1-3*10 6 cells / ml, store the cell cryopreservation solution containing the centrifuged cells in a cryopreservation tube, and place the cryopreservation tube at a temperature of 3-4°C for 10-15 minutes for pre-cooling;

[0043] S2: Preliminary freezing: Take out the cryopreservation tube pre-cooled in step S1 and store it at -20°C for 30-40 minutes for preliminary freezing;

[0044] S3: Second...

Embodiment 3

[0046] The specific steps of the cryopreservation method of the cell cryopreservation solution are as follows:

[0047] S1: Pre-cooling: The composition of the cell freezing solution contains 18 mg of fetal bovine serum, 8 mg of dimethyl sulfoxide, and 74 mg of 1640 culture medium per 100 mg according to the weight ratio. Replace the cryopreservation medium with a new cell cryopreservation medium, select exponentially growing cells for centrifugation, obtain the centrifuged cells and place them in the cell cryopreservation medium, the cell content in the cryopreservation tube is 1-3*10 6 cells / ml, store the cell cryopreservation solution containing the centrifuged cells in a cryopreservation tube, and place the cryopreservation tube at a temperature of 3-4°C for 10-15 minutes for pre-cooling;

[0048] S2: Preliminary freezing: Take out the cryopreservation tube pre-cooled in step S1 and store it at -20°C for 30-40 minutes for preliminary freezing;

[0049] S3: Secondary long-...

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Abstract

The invention belongs to the technical field of cell cryopreservation, and particularly relates to a cell cryopreservation solution and a cryopreservation method. The cell cryopreservation solution comprises fetal calf serum, dimethyl sulfoxide and a 1640 culture solution; the cryopreservation method of the cell cryopreservation liquid comprises the following specific steps: S1, precooling: selecting cells growing in an exponential state, centrifuging, obtaining centrifuged cells, putting the centrifuged cells into the cell cryopreservation liquid, storing the cell cryopreservation liquid containing the centrifuged cells through a cryopreservation tube, and precooling the cryopreservation tube at the temperature of 3-4 DEG C for 10-15 minutes; s2, primary cryopreservation: taking out the precooled cryopreservation tube in the step S1, and storing the taken-out cryopreservation tube at the temperature of -20 DEG C for 30-40 minutes for primary cryopreservation; and S3, performing secondary long-term cryopreservation. Through the cooperation of fetal calf serum, dimethyl sulfoxide and a 1640 culture solution, the prepared cell cryopreservation solution has small damage to cells, andthe survival rate of frozen cells is high.

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a cell cryopreservation solution and a cryopreservation method. Background technique [0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in -196°C liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderately preserving a certain amount of cells can prevent the cells from being lost due to contamination of the cells being cultured or other accidents, which plays a role in cell preservation. [0003] The existing cell cryopreservation method leads to low cell survival rate, thereby reducing the effect of cell cryopreservation. Contents of the invention [0004] The purpose of the present invention is to provide a cell cryopreservation sol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0252
Inventor 吴亚剑杨锋闵雨蒙张素芳
Owner 西部医学科技集团有限公司
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