250results about How to "Meet the experimental needs" patented technology

The present invention relates to a time / digital converter based on FPGA and its conversion method, belonging to the field of time measurement technology. Said converter includes the following components: fine time measurement unit, coding unit, coarse time measurement unit and data buffer storage unit. The main application of said invention is to make high accuracy time measurement, it can be used in the fields of basis research and various application researches.

The invention provides a high-precision and high-integrality time-digital converter based on an FPGA (Field Programmable Gate Array), and an implementation method. The time-digital converter comprises a fine time measuring unit, wherein the fine time measuring unit consists of a time alternate sampling unit, a multi-stage sampling data buffering unit and a snapshot and encoding unit. The invention aims to provide the high-integrality precision time-digital converter based on an FPGA. Innovation points lie in that: a fine time measuring part adopts a technology of a latch snapshot and rapid encoding combined architecture based on phase splitting clock alternate sampling, multi-stage sampling data caching and cached multi-stage sampling data. The high-precision and high-integrality time-digital converter has the characteristics of simple structure, design flexibility, high portability, short measuring dead time, large dynamic range, low cost, flexible interface and the like, and can be applied to the fields of aerospace, space research, communication, biomedicine, earth dynamics, relativity research and the like.

The invention provides a method and device for detecting the rotation angle of a vehicle steering wheel based on an image, and belongs to the technical field of automobiles. The steering wheel is marked, and then a marking image is obtained and identified, so that the rotation direction of the steering wheel and the rotation angle of the steering wheel are judged. By the method, the rotation angle of the steering wheel can be detected in real time, the detection result is accurate, and when the rotation angle of the steering wheel is more than one circle, the rotation angle of the steering wheel is also accurately obtained; by the device, sight of a driver for observing an instrument is not blocked; and the device is convenient to install and demount and strong in portability, and experimental requirements of conventional vehicles are met.

The invention relates to an experiment device and method for simulating drilling-production double-work-condition pit shaft sand carrying multi-phase flowing. The experiment device consists of a gas supply system, a liquid supply system, a sand injection system, a test pipe section, a separation recovery system and a monitoring system, wherein the test pipe section consists of a transparent inner tube and an outer tube; and the inner tube is dismountable, and is arranged in the outer tube for forming a concentric annular space. The experiment device can be used for meeting the test requirements of six different experiments on flow tests and sand sinking rule tests of gas drilling sand-carrying gas flow, slurry drilling sand-carrying fluid, foam drilling sand-carrying multi-phase flow, sand discharge gas well sand-carrying gas flow, sand discharge oil well sand-carrying oil flow and sand-carrying multi-phase flow when sand-discharge gas well stratum releases water in horizontal and vertical pit shafts under two kinds of work conditions including the drilling work condition and the production work condition; the functions are comprehensive; and economy and practicability are realized.

The invention discloses a natural gas hydrate saturation and sediment permeability synchronous measurement device. The device comprises an inverted reaction kettle, wherein a vacuum pumping system, a confining pressure control system and a prepared sample circulation system are connected to the bottom end of the reaction kettle, a permeability transient measurement system, a hydrate saturation measurement system and a data collection system are connected to the top end of the reaction kettle and connected with a computer, and the reaction kettle, the prepared sample circulation system and the permeability transient measurement system are all arranged in a temperature control system. The invention also discloses a natural gas hydrate saturation and sediment permeability synchronous measurement method. Through the device and method, synchronous transient measurement of the hydrate saturation and sediment permeability in the synthesis and decomposition process can be realized, and a reliable experimental platform is provided for research on the change law of reservoir permeability in the hydrate reservoir-forming and exploiting process.

The invention discloses a nuclear reactor pressure regulator safety valve water seal test system and method. A circuit can carry out low-pressure and medium-pressure hydraulic water seal experiments under vacuum, air and multi-component non-condensable gas, and reveals the water seal process and the hydraulic water seal mechanism phenomenon of the hydraulic water seal structure. The circuit mainlyincludes a deionized water system, a heating water tank, a hot water pump, a pressure vessel, a vacuum pump, a non-condensable gas, an exhaust pressure relief port, a water seal experimental section,and a control and acquisition system. The experimental pressure range is from 0.1 to 1.0 MPa and the temperature range is 0 to 180 degrees centigrade. The experimental circuit of the present invention can realize the visualization of the experimental phenomenon of the water seal structure. The circuit pressure vessel is designed in an integrated manner and has multiple sensing devices. The systemand method have the advantages of high safety, high precision of experimental data, and easy installation and maintenance.

The invention relates to a method for testing the vortex-induced vibration of a suspended flexible standpipe based on an open channel experiment water tank. The method employs the open channel experiment water tank and comprises the following steps of: (1) placing an experiment standpipe; (2) constructing a water flow environment of the open channel experiment water tank; (3) placing a testing instrument; (4) testing the velocity profile of a shear flow; and (5) testing the vibration response of the flexible standpipe. The water tank bottom slope, the water flow, the water depth, the velocity profile of the shear flow, the flexible standpipe material and size can be adjusted according to an experiment requirement. The method captures the vibration displacement of a mark point on the flexible standpipe by using two high-speed video cameras so as to achieve non-intervention interference-free flexible standpipe vibration testing under a shear flow velocity profile condition.

The invention discloses a volatile organic compound (VOC) collecting system used in the wood heat treatment process and a collecting method utilizing the VOC collecting system. The VOC collecting system comprises a heat treatment device, a gas condensation device, a gas drying device, a VOC absorption device, a gas circulation device and a deoxygenation device which are sequentially connected through connecting pipelines. The VOC absorption device is composed of a VOC liquid phase absorption device and a VOC solid phase absorption device which are connected in series and used for absorbing VOC released in the heat treatment process. The VOC released by wood heat treatment is cooled and dried and then enters the VOC absorption device. After the VOC is collected, residual gas returns to a wood high-temperature heat treatment device through the deoxygenation device, and circulation is conducted until the VOC is completely absorbed. The VOC collecting system is simple, the collecting method enables operation to be convenient, process conditions are easy to control in the collecting process, and the collecting system and method are suitable for collecting released VOC of various types of wood under different heat treatment conditions.

The invention discloses a big gecko's vertebral column three-dimensional positioning device and a big gecko's vertebral column three-dimensional positioning method applied to big gecko in-vivo spinal cord preparation and electrophysiological related experiments thereof, and belongs to the technical field of positioning. According to the method, by using the characteristic that a concave arc structure between the front and rear articular processes of the cervical vertebra, the thoracic vertebra and the lumbar vertebra of a big gecko can be used for clamping and positioning, a clamping device is designed to clamp two adjacent vertebras, so that different zones of the vertebral column of the big gecko are reliably positioned. The device consists of a supporting seat, a moving block, a vertebra clamp, a set screw and a locking screw. The device is reliable in positioning and easy to operate, fills the blank that a special vertebral column three-dimensional positioning device for gecko reptiles does not exsit at home and abroad, and lays a foundation for the big gecko in-vivo spinal cord preparation and electrophysiological experimental study thereof.

The invention discloses a sound emission probe precise positioning device of a rock uniaxial test and a use method. The device comprises an annular dial plate with a positioning hole and an annular nonius track, a nonius with a threaded hole and a set screw, a probe containing cap with a screw rod, and a scale bar; the nonius determines an orientation according to the annular dial plate; the annular dial plate is connected with the positioning hole by the scale bar; the scale bar has the effects of connecting and determining the position of the dial plate; one end, pointing to the inner side of the annular dial plate, of the screw rod is connected with the probe containing cap, and a sound emission probe is fixed by a spring (screw); during mounting, the screw rod is adjusted to increase frictional force between the probe and a rock test piece, and the probe and the positioning device are mounted on the surface of the rock test piece by using the frictional force. The device is flexible and convenient, and can perform sound emission probe precise positioning and fixing on rock test pieces with different shapes and sizes, meanwhile, the number and mounting orientation of the probe can be selected, and the time and vigor of researchers are effectively saved.

The invention belongs to the technical field of material analysis and testing, and relates to a manufacturing method of an in situ double-tilt electronic microscope sample rod for which an electric field and a light signal can be added externally; the method comprises the following steps that: first, three independent channels are arranged in a hollow copper bar of a sample rod based on an existing single-title electronic microscope sample rod in an insulated and closed way; the three independent channels are respectively distributed to a horizontal transmission rod, a lead and an optical fiber and are insulated and sealed; the sample rod tilts rotationally along a Y axis through a stepper motor which is arranged outside the sample rod and is connected with the horizontal transmission rod; then an electric field terminal is arranged on a sample cup and is more than 10cm away from an optical axis, so that the concentration of charge and the affection of the imaging quality are prevented; then an optical fiber fixed column is arranged on the sample cup, metal is sprayed onto the surface of a transmitting optical fiber and the optical fiber is connected in, and a laser led-in sample is formed into an in situ transmission double-tilt sample rod; and according to the preparation method of the in situ double-tilt electronic microscope sample rod, a design idea is novel, the used equipment and technologies are mature, the manufacturing cost is low, the market blank is filled, experimental needs are met, and the practicality and test results are good.

The invention discloses a simulation experiment device for hydrostatic stress corrosion electrochemical action of a magnesium alloy and an experiment method of the simulation experiment device. The simulation experiment device comprises an electrolyte tank, a test device, a computer terminal and an electrochemical workstation, wherein the test device is placed in the electrolyte tank; electrolyte in the electrolyte tank is forced by a high-precision peristaltic pump to cyclically crawl. The simulation experiment device is simple in overall structure, and static pressure can be continuously adjusted. The experiment method is to adjust a screw rod of the test device to apply different sizes of hydrostatic stress on a magnesium alloy test sample, and then adjust the flow rate of the electrolyte through the peristaltic pump to really simulate a living body microenvironment in vitro, so as to carry out a series of electrochemical test operations on open circuit potential, a polarization curve, an alternating current impedance spectrum and electrochemical noise; test results are real and stable, and the reappearance and the reproducibility are high. The simulation experiment device and the simulation method can fully meet the requirement of the magnesium alloy for relevant research use on bearing of the hydrostatic stress in a human bone microenvironment.

The invention discloses a method for synchronously determining the content of trihalomethane and haloacetic acid in drinking water, relates to a method for determining the content of trihalomethane and haloacetic acid in the drinking water, and aims at solving the problems that in the prior art, synchronous pretreatment cannot be performed on trihalomethane and haloacetic acid in the drinking water, and time and labor are consumed and the cost is high when trihalomethane and haloacetic acid in the drinking water are synchronously determined and separately determined. The method comprises the steps that 1, a water sample to be determined is acidized; 2, the polarity of the water sample to be determined is enhanced; 3, extracting is performed; 4, deriving is performed; 5, neutralizing is performed; 6, the peak area of trihalomethane in the water sample to be determined is determined; 7, the peak area of haloacetic acid in the water sample to be determined is determined; 8, a standard curve is drawn; 9, the concentration of trihalomethane in the water sample to be determined is calculated; 10, the concentration of haloacetic acid in the water sample to be determined is calculated. The method for synchronously determining the content of trihalomethane and haloacetic acid in the drinking water can be achieved.

The invention discloses a design method for an annular structural member diffusion bonding fixture. A proper fixture material is selected, and a fixture is guaranteed to be safely used while an expansion pressure difference which is large enough is provided. In order to guarantee to generate even and stable connection pressure on a connection interface, a fixture structure and an annular member structure are corresponded, and detail characteristics are similarly matched; the inner wall of the fixture is in complete contact with the outer surface of a connection member as far as possible; and the outer wall of the fixture is convenient in manufacture, saves cost and is designed to be regular cylindrical surface. The coefficient of thermal expansion of an annular fixture manufacture materialis reasonably selected, since the expansion coefficient of the fixture material and the expansion coefficient of specimen alloy are similar in a cooling process, a cooling shrinking difference is small, and an influence on a connection interface is small. By use of the technical scheme of the invention, the successful connection of TiAl alloy and Ti2AlNb alloy is realized, and a reference is provided for the diffusion bonding of other annular structural members with a large outer layer metal expansion coefficient.

The invention discloses a lithium ion battery thermal runaway spreading simulation method and device. The invention relates to a lithium ion battery thermal runaway spreading simulation method and system. The method comprises the following steps: firstly, establishing a lithium ion battery normal heat generation prediction model and a lithium ion battery thermal runaway heat generation predictionmodel; setting a simulation monomer for simulating the lithium ion battery based on a wireless energy transmission principle of a primary side and a secondary side; then, heat generation estimation iscarried out on the lithium ion battery by utilizing the established heat generation model; calculating the heat generation power by utilizing heat generation estimation; the heat generation current is calculated by utilizing the heat generation power of the lithium ion battery; calculating expected values of corresponding parameters of a primary side by taking the calculated heat generation current of the lithium ion battery as a secondary side current demand value; corresponding parameters of the primary side are adjusted to simulate heat generation of the heat generation element; and judging thermal runaway spreading through the obtained surface or internal temperature distribution of the simulation monomer to complete lithium ion battery thermal runaway spreading simulation verification, thereby evaluating the safety of the lithium ion battery system and realizing lithium ion battery module thermal runaway management and control experiment verification.

The invention discloses a lower hybrid wave power control system in a Tokamak experiment, which comprises 24 high-power klystrons. The output end of each high-power klystron is provided with a directional coupler. Incident power coupled by the directional coupler is sent to a detector after direct current (DC)-blocking through a DC-block and filtering through a filter, detection voltage coming out of the output end of the detector is sent to an amplifier, amplified voltage output by the amplifier is collected through an analog input module, processed through a power control lower computer, and output by an analog output module, and the output end of the analog output module is connected to a personal identification number (PIN) electrically controlled attenuator of a microwave preceding stage. By means of collection of the power control lower computer, PID algorithm and output of the analog input module, the lower hybrid wave power control system in the Tokamak experiment controls the PIN electrically controlled attenuator of the preceding stage and controls lower hybrid wave preceding stage power so that output power of the klystrons is controlled. Meanwhile, by means of collection of output power and status signals of the klystrons, working status of the system is judged, PID control is adjusted according to requirements so as to avoid output overshoot of the klystrons, ensure stability of the lower hybrid wave system, and meet requirements of the Tokamak physical experiment. The power control lower computer of the system adopts a cRIO system, and enables the control system to have accuracy and reliability of a field programmable gate array (FPAG) and flexibility of an ordinary industrial personal computer.

The invention provides a stratum core holder. The stratum core holder comprises a holder cylinder, a rubber sleeve, a rubber sleeve end plug, cylinder covers and a sealing assembly, wherein the holder cylinder is of a hollow structure, and a core sample in arranged at the center position of the holder cylinder; the rubber sleeve end plug comprises an outer end plug body and inner end plug bodies; the rubber sleeve and the inner end plug bodies are arranged in the holder cylinder, and the periphery of the core sample is sleeved with the rubber sleeve; the inner end plug bodies are embedded to the two ends of the rubber sleeve and make direct contact with the core, and the inner end plug bodies and the rubber sleeve are matched to seal and wrap the core sample; in addition, the two ends of each inner end plug body are connected with metal pipe lines, and the other end of each metal pipe line is connected with a cylinder end cap and extends out of the holder cylinder; the metal pipe lines are fixed through sealing pressing caps. The stratum core holder can meet the experiment requirements of exerting static pressure on cores with different diameters, and is convenient to mount and dismount.

The invention discloses a hydraulic system for a torque test worktable and a control method of the hydraulic system. The hydraulic system comprises an oil tank and two hydraulic oil circuits for controlling the movement of hydraulic cylinders; both of the two hydraulic oil circuits for controlling the movement of the hydraulic cylinders are connected with the oil tank, wherein each hydraulic oil circuit comprises an oil absorption filter, an oil pump motor, a variable displacement piston pump, a one-way valve, a high pressure filter, a pressure switch, a pressure gauge, a pressure sensor, an energy accumulator, an integrated valve group, a safety protection valve group and one of the hydraulic cylinders, and the integrated valve group is composed of a proportional overflow valve, a proportional directional valve, a hydraulic control one-way valve and an oil circuit valve block; the oil absorption filter is placed in the oil tank; and the oil absorption filter, the variable displacement piston pump, the one-way valve, the high pressure filter, the integrated valve group, the safety protection valve group and the hydraulic cylinder are sequentially connected in series. According to the hydraulic system, an acting force of each hydraulic cylinder is applied to two ends of a mechanical rotating moment arm by virtue of a pull force (a thrust force) to form a torque required by a test, so that the test requirement is met.

The invention discloses a manufacturing method of a lamellar anisotropic rock similar material, and belongs to the technical field of rock surveying. Common cement, river sand, water and other raw materials are casted into a mould so as to form a mold, the lamellar anisotropic rock similar material is manufactured and is then cut into an international-standard cylinder sample, and a triaxial testis carried out. The manufacturing method is simple and fast, the materials are wide in source, low in cost and good in forming effect, and the rock parameters can be adjusted conveniently.

The invention discloses a separation culture method of the primary hepatocyte of a jian carp. The separation culture method comprises the following steps: selecting a healthy jian carp without injury, collecting blood from the caudal vein of the jian carp, then sterilizing the surface of a fish body, placing into a super clean bench, and aseptically collecting the liver of the jian carp; rinsing by using a PBS solution which contains double antibodies, adding a trypsin digestion solution, wherein the temperature of digestion treatment is 26-28 DEG C, and the time of the digestion treatment is 15-20 minutes; after digestion is finished, adding to a culture medium A to finish the digestion, filtering the trypsin digestion solution by using a filter screen of 200 meshes, and collecting filter liquor; and respectively centrifugalizing 50 grams of the filter liquor and 30 grams of the filter liquor for 5 minutes, then washing twice by using a culture medium B, removing a supernatant to obtain a precipitation, namely an extracted hepatocyte, preparing a cell suspension, and planking for culture. The experimental method disclosed by the invention is simple and fast and can save the separation time to a great extent. The separation time adopted by an experiment is about 40 minutes, and the obtained hepatocyte has the advantages of good growth condition, small erythrocyte quantity and cell survival rate of about 95%.

The invention relates to the field of hydraulic experiments, in particular to an experimental device and an experimental method for researching water flow characteristics of multiple types of river channel intersection ports. The aim is that the experimental device and the experimental method can carry out experiments on water flow characteristics of different types of river channel intersection ports, and are characterized by being easy to operate and being high in reutilization. The technical scheme is that the experimental device suitable for researching water flow characteristics of multiple types of river channel interaction ports is characterized by comprising a Y-shape symmetric river channel intersection experiment unit, two straight river channel interaction experiment units, a bending river channel and straight river channel intersection experiment unit, a water supplying pool set, and an external tank. The experimental method utilizing the experimental device for researching water flow characteristics of multiple types of river channel intersection ports includes the following steps: step 1, determining an experiment zone of intersection of three types of river channels; and step 2, conducting research on many issues through the structure of the external tank.

The invention relates to a tunnel environment quantitative simulation system and a use method thereof. The experiment system mainly comprises a control device, a camera scanning experiment platform and an environment adjustment device, and the environment adjustment device comprises a brightness adjustment unit, a humidity adjustment unit and a dust adjustment unit. The experiment system of tunnel face information collection equipment on environmental factor sensitiveness in a tunnel is developed, the experiment system changes main factors such as illumination intensity, dust density and water vapor humidity which influence equipment operation by simulating real environment characteristics of the tunnel, sensitiveness of experiment equipment on all influence factors is obtained, and therefore tunnel structure surface collection equipment accessory selection and relative parameter adjustment are guided to improve the reliability and practicability of research and development equipment. According to the tunnel environment quantitative simulation system and the use method thereof, the structure is simple, operation is easy and convenient, and the work efficiency is improved.

The invention relates to the technical field of outer window and curtain wall waterproofness detection, particularly to on-site water spraying detection equipment for building external windows and curtain walls. The equipment includes a lifting assembly, a frame body which is driven by the lifting assembly to vertically move outside of a building body, a spraying piece which is installed on the frame body and connected to a water source, a sucking disc assembly which is arranged on the frame body so as to adsorb and fix the frame body to a building external window or curtain wall, and an angleadjusting and positioning assembly that is arranged between the frame body and the sucking disc assembly. The equipment provided by the invention has the effect of facilitating the water spraying detection by workers on building external windows or curtain walls on site.

The invention discloses a seed screening device for agricultural experiment seedling. The seed screening device comprises a screening box; a first motor is arranged on the inner wall on one side of the screening box; a thread screw is arranged on the outer wall at one end of an output shaft of the first motor; a thread sleeve is connected onto the outer wall of the thread screw; an arc-shaped plate is arranged on the outer wall at the top of the thread sleeve; fixing plates are arranged on the inner walls on two side of the screening box; a sliding chute is formed in the inner wall on one sideof each fixing plate; two first pulleys are connected onto the inner wall at the bottom of each sliding chute in a sliding manner; supporting legs are arranged on the outer walls at the tops of the two first pulleys; and the outer walls at the tops of the supporting legs are separately connected with first connecting plates and second connecting plates. Air generated in an air blower is blown inthe screening box by means of an air outlet pipe, dried seeds can be effectively distinguished from full seeds under wind force, the seeds are effectively screened, the screening effect is good, and the working efficiency of people is improved.

The invention discloses a micro flapping-wing air vehicle experimental platform and a flight data acquisition method based on the same. The platform at least comprises a six-axis force sensor, a frequency meter, an oscilloscope and an upper compute. That measurable flight data include aerodynamic force, aerodynamic moment, flapping wing frequency and flapping wing power, which can meet the experimental requirements of a flapping wing type aircraft to the maximum extent and simulate a real flight state. The fixed bracket of the aircraft is designed as a mouth-shaped structure, which can be widely used in the flight test of micro flapping-wing aircraft with different sizes. The platform also comprises an attitude conversion joint, which can realize the multi-attitude experiment test of the micro flapping-wing aircraft. The fixed base of the invention supports three-degree-of-freedom position adjustment, and can realize multi-angle and multi-orientation adjustment of the photoelectric sensor.

The invention discloses a device and a method for measuring the actual maximum torque of a motor. The device comprises a mounting assembly, which is used for installing the measured motor; a transmission mechanism, wherein the input end of the transmission mechanism is connected with the measured motor, and the output end is connected with the pulley block and, the transmission mechanism is used for driving the rotary motion of the measured motor to be transmitted into the pulley block; a weight part, which is connected with the output end of the transmission mechanism and is suspended below the pulley block. The invention has the beneficial effects that the supporting component, the transmission component, the pulley block and the weight part are ingeniously matched; measurement of the ultimate torques of the motor at different speeds is realized with the simple device and at a low cost in a limited condition; high-cost equipment such as a torque measuring instrument, a torque sensorand the like is not needed; complicated modification does not need to be carried out on different motor test structures, and the requirements of general small enterprises and school experiments can bewell met.

The invention discloses a constant temperature-constant humidity laboratory, which belongs to the field of laboratories. A workshop, an equipment room, an air feeding bin and an air mixing bin are arranged in the laboratory, the top of the workshop is provided with a porous plate suspended ceiling, an air feeding pipe is arranged in the air feeding bin, a fresh air hole introduces fresh air into the air mixing bin by a fresh air pipeline, an air conditioner unit is arranged in the equipment room, the lower part of the air mixing bin is connected to the air conditioner unit, the air conditioner unit is connected to the air feeding pipe by an air conditioner auxiliary electric heater, a plenum chamber and a humidifier, air returning upright columns are arranged in the workshop, the lower parts of the air returning upright columns are provided with air returning holes, and the air returning upright columns are connected to the air mixing bin by air returning pipelines. The constant temperature-constant humidity laboratory also comprises an intelligent control system having a PLC. Compared with the prior art, the constant temperature-constant humidity laboratory has the characteristics of high airflow circulation strength, stable wind speed, uniform temperature and humidity, no dust accumulation, good stability, capability of meeting a fresh air volume demand of workers, high disturbance resistance, high control precision and direct and visible temperature and humidity changes in the laboratory.

The invention discloses a plugging and unplugging effect evaluation method for a plugging material. The method comprises the steps of S1 preparing a plurality of core columns with different crack widths, the core columns are made of stainless steel, and the crack width is in a range of 0.05 to 8 mm; S2 injecting a liquid plugging material into the crack of one of the core columns, maintaining under the simulated formation temperature and pressure condition, and obtaining the core column solidified by the liquid plugging material after the maintenance is completed; S3 carrying out plugging evaluation on the core column prepared in the step S2 by adopting a dynamic damage evaluation instrument; S4 performing a dynamic acid dissolution experiment on the core column prepared in the step S2 to obtain the mass, the acid dissolution depth and the gas logging permeability of the core column after acid dissolution; and S5 carrying out a static acid dissolution experiment on the core column or the consolidation block of the liquid plugging material prepared in the step S2, and recording the acid dissolution soaking time and the mass of the core column or the consolidation block after acid dissolution. According to the invention, the plugging and unplugging effects of the plugging material can be evaluated with high precision.

The invention relates to the field of biotechnology, in particular to a monoclonal cell strain of a human embryo kidney cell 293T-C10 for packaging viruses as well as a screening method of the monoclonal cell strain. 293T cell mycoplasmas are removed, monoclonal screening is carried out during logarithmic growth period to obtain uniform monoclonal cells, different transfection and virus removal efficiencies are compared, and screening is carried out to obtain the monoclonal cell strain. Such cell strain has a collection number of CCTCC C201281, is very high in transfection efficiency and virus packaging stability, is remarkably improved in virus removal efficiency of 293T cells than that before screening, and can infect a majority of tumors and other cells to be infected without ultracentrifugation, so as to meet most of experimental demands.

The invention provides a supercritical water particulate matter visual measurement experimental device and method. The device comprises a preheating section for primarily heating fluid in a pipeline,a heating section for secondarily heating the fluid in the pipeline, a visual section for observing particulate matters, a cooling section for cooling the fluid in the pipeline, a pressure stabilizingsection for adjusting the pressure of a loop and a feeding section for adding water and particulate matters into the loop. The experimental device can study the flowing condition of fluid in the pipeline and the deposition movement condition of particulate impurities in the fluid in a visual measurement mode, is visual in observation, can accurately and effectively measure related data, and meetsexperimental requirements and engineering application.