The invention discloses a gene-edited tobacco one-way liquid-conveying and multi-plant automatic hydroponic device. The device comprises a liquid conveying metering pump (1), a liquid input main pipe (2), a liquid output main pipe (3), liquid input branch pipes (4), liquid output branch pipes (5), a field planting plate (6), single-plant hydroponic units (7), hydroponic cup liquid level monitoring sensors (8) and a liquid level control computer. The one-way liquid-conveying hydroponic device is constructed, each plant hydroponic device is relatively independent and communicates with the whole hydroponic system through one-way input and output of a nutrient solution, independent use of the nutrient solution is achieved, and cross infection of tobacco diseases due to nutrient solution exchange is prevented; meanwhile, each liquid level monitoring sensor monitors the water level in real time and is matched with a conveying pump, and automatic water culture of plants is achieved; and the equipment is low in cost and simple to operate, can realize automatic planting, saves a large amount of labor, and can be directly used for screening of tobacco gene editing materials and factory-like hydroponic planting.