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Culture medium and method for culturing tissues of Chinese cymbidium faberi rolfe

A technology of tissue culture and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high medicinal cost and labor cost, complex formula, etc., and achieve the reduction of drug cost and labor cost, pollution rate The effect of lowering, step reduction

Inactive Publication Date: 2010-11-24
YANGZHOU UNIV
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Problems solved by technology

[0009] In order to overcome the shortcomings of the existing Cymbidium tissue culture formula which is complex, budding and rooting need to be carried out step by step, and high medicinal and labor costs, the present invention provides a Cymbidium protocorm or rhizome tissue culture for one-step seedling growth. Formula, using this formula can achieve one-step seedling growth, which effectively solves the problem

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  • Culture medium and method for culturing tissues of Chinese cymbidium faberi rolfe

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Embodiment 1

[0027] After the Cymbidium variety 'Laojipin'×'Taisu' is obtained, the seeds are cultivated on the germination medium for 8-10 months, and after protocorms or rhizomes are obtained, cultivate according to the method of the present invention:

[0028] 1) Choose healthy green protocorms or rhizomes as materials;

[0029] 2) Prepare medium: use Huabao No. 1 as the basic medium, add 0.5mg / L IBA+0.2mg / L NAA and add 30g·L sucrose -1 , agar 4g·L -1 , activated carbon 1g L -1 , coconut milk 10% (V / V), pH is 5.6, is packed in the Erlenmeyer flask at 121 ℃ and sterilized for 20min;

[0030] 3) Inoculation: inoculate the material selected in step 1) on the medium of step 2), try to connect with blocks without cutting or breaking; connect 6-8 protocorms per bottle ( figure 1 );

[0031] 4) Culture conditions: culture in a culture room at 25±2°C, with light of 2000-3000lx;

[0032] About 2 months after inoculation, some sprouts, 3 months, the buds grow taller, bloom leaves, some parts...

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Abstract

The invention relates to the field of culturing tissues of plants, in particular to a formula of a culture medium and a method for culturing the tissues of Chinese cymbidium faberi rolfe. In the culture medium of the invention, Hyponex No.1 is taken as a basic culture medium, and 0.5 mg / L lebutyric acid, 0.2 mg / L naphthylacetic acid, 30 g / L sucrose, 4 g / L agar, 1 g / L activated carbon and 10 volume percent coconut milk are added into the basic culture medium, wherein a pH value is 5.6. In tissue culture, a selected healthy protocorm or root stock is inoculated to the culture medium and is cultured to be a seedling. The method has the advantages of simple operation, one-step seedling culture, good practical effect and capacity of reducing medicament cost, labor cost and pollution ratio and shortening the period of producing and breeding seedlings of the Chinese cymbidium faberi rolfe.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a medium formula for Chinese Cymbidium tissue culture and a method for cultivating protocorms or rhizomes using the medium. Background technique [0002] New varieties of Cymbidium mainly rely on selection from wild plants. So far, the wild resources of Cymbidium are on the verge of extinction in Jiangsu, Zhejiang, and Anhui provinces. In the National Wildlife Conservation and Nature Reserve Construction Project officially launched at the end of 2001, orchids were listed as one of the 15 wild animal and plant groups and included in the focus of project construction, and all orchids were included in the "National List of Key Protected Wild Plants (Second Batch), thus included in the scope of protection of the Regulations of the People's Republic of China on the Protection of Wild Plants. Regulations prohibit commercial international trade in wild-sourced orchids. [0003] Onl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陶俊孔芬俞丽琴
Owner YANGZHOU UNIV
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