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Tissue culture rapid propagation method of cymbidium faberi

A technology of tissue culture rapid propagation and subculture, which is applied in the field of plant tissue culture, can solve the problems of long reproduction cycle, low value-added rate, and poor quality of tissue culture seedlings, and achieve the effect of rich nutrition and increased disinfection

Inactive Publication Date: 2018-03-30
GUANGXI YIMU AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because each orchid variety has its own characteristics on the habit, when explants of Cymbidium pedicel buds are used for tissue culture, the existing explant disinfection method and common medium for tissue culture cannot be obtained. Satisfactory tissue culture results, resulting in long propagation cycle, low value-added rate, and poor quality of tissue culture seedlings

Method used

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Examples

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Effect test

Embodiment 1

[0029] A method for tissue culture and rapid propagation of Cymbidium, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0030] (1) Disinfection of explants: with the Cymbidium that is blooming, growing robustly and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 1min, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 8min and then continue to peel off one layer of bracts, then continue soaking in the plant disinfectant for 5min, use sterile water 3 times to obtain the sterilized explants; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.05 part of glycyrrhizic acid, 0.8 part of pomegranate peel extract, 0.5 part of ...

Embodiment 2

[0035] A method for tissue culture and rapid propagation of Cymbidium, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0036] (1) Disinfection of explants: with the Cymbidium that is blooming, growing robustly and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of 75% alcohol soaked for 1.5min, then peeled off the bracts on the pedicel bud surface, then continued to peel off one layer of bracts after soaking in the plant disinfectant for 12min, then continued to soak for 8min in the plant disinfectant, and used Bacterial water 4 times, get the explant after the disinfection; Described plant disinfectant is mixed by the raw material of following weight parts: 0.06 part of glycyrrhizic acid, 1.0 part of pomegranate peel extract, 0.6 p...

Embodiment 3

[0041] A method for tissue culture and rapid propagation of Cymbidium, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0042] (1) Disinfection of explants: with the Cymbidium that is blooming, growing robustly and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 2 minutes, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 15 minutes and then continue to peel off one layer of bracts, then continue soaking in the plant disinfectant for 10 minutes, use aseptic water 5 times to obtain the sterilized explants; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.08 parts of glycyrrhizic acid, 1.2 parts of pomegranate peel ex...

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Abstract

The invention provides a tissue culture rapid propagation method of cymbidium faberi, and belongs to the technical field of plant tissue culture. The method comprises the steps of disinfecting of pedicel bud explants, inducing culture, subculture, strong seedling culture and the like, wherein, the explants are disinfected through a method of matching alcohol disinfection and plant disinfection solution. Active ingredients of the plant disinfection solution are glycyrrhizic acid, pomegranate peel extract and herba houttuyniae extract; the media used in each culture stage are added with okra juice and folium mori juice as nutrients. The method of the invention has little harm to the pedicel bud explants, and a medium formula provided by the invention can significantly improve induction differentiation rate of the cymbidium faberi, proliferation coefficient and rooting rate of the explants, shorten cultivation period, and improve quality of cymbidium faberi tissue cultured seedlings.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Cymbidium. 【Background technique】 [0002] Cymbidium is a ground herbaceous plant; it has inconspicuous pseudobulbs, 5-8 leaves, which are band-shaped, relatively strong upright, about 25-80 cm long, 7-12 mm wide, and the base is often folded in half V-shaped, the veins are relatively translucent, and the edges are often thickly serrated. Cymbidium is one of the oldest and most popular orchids in China. It is a national second-level key protected wild species and one of the more cold-resistant orchid varieties. For a long time, Cymbidium has been mainly propagated by ramets, and the propagation speed of ramets is relatively slow. If you want to get more plants in a short time, you can use tissue culture to propagate. [0003] At present, many researches have been done on the tissue culture of Cymbidium at home ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金显陈财宝
Owner GUANGXI YIMU AGRI TECH CO LTD
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