A kind of rapid propagation Cymbidium cymbidium tissue culture method

A tissue culture, Cymbidium orchid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of unsuccessful induction of protocorms, poor quality of regenerated seedlings, low seed germination rate, etc., and achieve improvement The effect of induction rate, emerald green color and short incubation time

Active Publication Date: 2022-03-08
宜宾云朵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because the seeds of Cymbidium are not fully developed and have almost no endosperm, under natural conditions, seed germination needs to be symbiotic with fungi, so the seed germination rate is extremely low, and the traditional branch reproduction is extremely slow. In order to quickly reproduce Cymbidium in large quantities, To achieve the industrialization of orchids, the technology of rapid propagation of plant tissue culture is applied in production. However, at present, this technology has problems such as unsuccessful protocorm induction, low induction rate, low value-added rate, long cultivation time, and poor quality of regenerated seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Cymbidium tissue culture was carried out according to the following method.

[0025] (1) Disinfection of explants: take the petals of Cymbidium grandiflora on the second day of flowering, rinse them with sterile water for 15 minutes, and soak them in phosphate buffer solution with a pH of 7.0 for 30 minutes (the preparation method of phosphate buffer solution is: take 0.2mol / L Potassium dihydrogen phosphate solution 250ml, add 0.2mol / L sodium hydroxide solution 118ml, dilute to 1000ml with water, shake well, and get it), then wipe with 75% alcohol for 30s, and then use 0.1% HgCl 2 Soak for 5 minutes, rinse with sterile water for 20 minutes, blot dry, cut into 0.8cm segments;

[0026] (2) Pseudoprotocorm induction: inoculate the explants obtained in step (1) into the first medium, and the medium consists of: WPM basic medium, 6-BA0.5mg / L, NAA1.0mg / L, Grape juice 5g / L, fish oil 5g / L, pH adjusted to 5.0, culture environment: culture temperature 25°C, daily light 12h, lig...

Embodiment 2

[0031] Cymbidium tissue culture was carried out according to the following method.

[0032] (1) Disinfection of explants: take the petals of Cymbidium grandis on the first day of flowering, wash them with sterile water for 15 minutes, and soak them in phosphate buffer solution with a pH of 7.0 for 30 minutes (the preparation method of phosphate buffer solution is: take 0.2mol / L Potassium dihydrogen phosphate solution 250ml, add 0.2mol / L sodium hydroxide solution 118ml, dilute to 1000ml with water, shake well, and get it), then wipe with 75% alcohol for 30s, and then use 0.1% HgCl 2 Soak for 5 minutes, rinse with sterile water for 20 minutes, blot dry, cut into 0.6cm segments;

[0033] (2) Pseudoprotocorm induction: inoculate the explants obtained in step (1) into the first medium, and the medium consists of: WPM basic medium, 6-BA0.5mg / L, NAA1.0mg / L, Grape juice 5g / L, fish oil 5g / L, pH adjusted to 5.4, culture environment: culture temperature 25°C, daily light 12h, light int...

Embodiment 3

[0038] Cymbidium tissue culture was carried out according to the following method.

[0039](1) Disinfection of explants: take the petals of Cymbidium grandis on the first day of flowering, wash them with sterile water for 15 minutes, and soak them in phosphate buffer solution with a pH of 7.0 for 30 minutes (the preparation method of phosphate buffer solution is: take 0.2mol / L Potassium dihydrogen phosphate solution 250ml, add 0.2mol / L sodium hydroxide solution 118ml, dilute to 1000ml with water, shake well, and get it), then wipe with 75% alcohol for 30s, and then use 0.1% HgCl 2 Soak for 5 minutes, rinse with sterile water for 20 minutes, blot dry, cut into 0.7cm segments;

[0040] (2) Pseudoprotocorm induction: inoculate the explants obtained in step (1) into the first medium, and the medium consists of: WPM basic medium, 6-BA0.5mg / L, NAA1.0mg / L, Grape juice 5g / L, fish oil 5g / L, pH adjusted to 5.4, culture environment: culture temperature 23°C, daily light 9h, light inten...

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PUM

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Abstract

The invention discloses a tissue culture method for rapidly propagating Cymbidium cymbidium. In the method, petals of Cymbidium cymbidium are used as explants, soaked in phosphate buffer solution for disinfection, and WPN culture medium containing grape juice and fish oil Protocorm induction was carried out, and proliferation was carried out in a specific DKW medium containing lecithin, followed by a specific B 5 Secondary rooting culture in the culture medium, planting seedlings are obtained by hardening and transplanting. This method has high induction rate, high multiplication multiple, short cultivation time, and the planting seedlings grow robustly and have emerald green color.

Description

technical field [0001] The invention belongs to the field of flower cultivation and propagation, and in particular relates to a tissue culture method for rapidly propagating Cymbidium grandiflora. Background technique [0002] Cymbidium hybridum (Cymbidium hybridum) is an evergreen perennial epiphytic herb of the genus Orchid. It is artificially cultivated by many generations of large-flowered epiphytic species, small-flowered weeping species and some ground orchids in the genus Orchid. Hybrid breeds. Cymbidium grandiflora has long green leaves, rough and magnificent flower posture, and is a world-renowned "orchid star". It has the elegant fragrance of Chinese orchid and the richness and variety of western orchid. It is very popular in the international flower market and is deeply loved by flower lovers. [0003] Because the seeds of Cymbidium are not fully developed and have almost no endosperm, under natural conditions, seed germination needs to be symbiotic with fungi, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刁银祥
Owner 宜宾云朵生物科技有限公司
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