High-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum
A technology of hybrid orchids and large flowers, applied in the field of plant tissue culture, can solve the problems of long flowering period, large number of flowering, lack of flower fragrance, etc., and achieve the effect of good flower quality, fast reproduction speed, and easy and precise control
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[0076] Embodiment 1, the tissue culture method of the high-efficiency seedling growth of the hybrid orchid of Chunjian 'Tonghai Jianlan' × Cymbidium 'KK560', carries out the following steps successively:
[0077] 1. Treatment of explants:
[0078] Select new shoots that are robust (3 to 6 cm long and without disease spots) from hybrid orchid plants, remove surface impurities and dirt, and bring them back to the laboratory, soak them in laundry detergent with a concentration of 10% to 15% (W / V) Wash in powder water, soak for about 1 hour, then rinse with running water for 1 hour, and put it on an ultra-clean workbench for sterilization: immerse the new sprouts in 75% (volume %) alcohol for 1 minute, and wash with sterile water 3 times (each time) 1 minute); peel off 1 to 2 outer bract leaves, immerse in 10% (V / V) sodium hypochlorite for disinfection for 10 minutes, wash with sterile water 3 times (each time for 1 minute); immerse in mercuric chloride disinfectant for 16 minutes...
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