High-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum

A technology of hybrid orchids and large flowers, applied in the field of plant tissue culture, can solve the problems of long flowering period, large number of flowering, lack of flower fragrance, etc., and achieve the effect of good flower quality, fast reproduction speed, and easy and precise control

Active Publication Date: 2021-10-08
HANGZHOU ACAD OF AGRI SCI
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  • Claims
  • Application Information

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Problems solved by technology

Chunjian is one of the representative varieties of orchids, mainly produced in the southwestern region of my country. Chunjian is verdant and green all the year round, its leaves are straight like swords, and its flowers are light and elegant. Based on white, yello...

Method used

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  • High-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum
  • High-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum
  • High-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum

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Embodiment 1

[0076] Embodiment 1, the tissue culture method of the high-efficiency seedling growth of the hybrid orchid of Chunjian 'Tonghai Jianlan' × Cymbidium 'KK560', carries out the following steps successively:

[0077] 1. Treatment of explants:

[0078] Select new shoots that are robust (3 to 6 cm long and without disease spots) from hybrid orchid plants, remove surface impurities and dirt, and bring them back to the laboratory, soak them in laundry detergent with a concentration of 10% to 15% (W / V) Wash in powder water, soak for about 1 hour, then rinse with running water for 1 hour, and put it on an ultra-clean workbench for sterilization: immerse the new sprouts in 75% (volume %) alcohol for 1 minute, and wash with sterile water 3 times (each time) 1 minute); peel off 1 to 2 outer bract leaves, immerse in 10% (V / V) sodium hypochlorite for disinfection for 10 minutes, wash with sterile water 3 times (each time for 1 minute); immerse in mercuric chloride disinfectant for 16 minutes...

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Abstract

The invention discloses a high-efficient seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum, which comprises the following steps: selecting new buds with strong growth vigor from the hybrid cymbidium, and treating to obtain sterilized small lateral buds and sterilized stem tips; performing induction culture on the sterilized stem tips on a protocorm induction culture medium, firstly inoculating the sterilized small lateral buds into a growth culture medium for re-greening growth culture, and then inoculating the small lateral buds into the protocorm induction culture medium for induction culture; dividing the obtained protocorm, and inoculating the protocorm into a subculture multiplication/differentiation culture medium for culturing; and cutting off the adventitious buds obtained by culturing, inoculating the adventitious buds into a rooting and seedling strengthening culture medium as buds, and culturing to obtain transplantable seedlings. The high-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum provided by the invention is suitable for tissue culture and rapid propagation of hybrid cymbidium varieties formed by taking the longibracteatum and the cymbidium hybridum as parents, and the problem that the traditional division propagation process is slow is solved.

Description

technical field [0001] The invention discloses a high-efficiency seedling-growing method for a hybrid orchid of Spring Sword×Cymbidium grandiflora, and the method relates to the field of plant tissue culture. Background technique [0002] Both Cymbidium longibracteatum and Cymbidium hybridum are herbaceous perennials belonging to the genus Cymbidium of the family Orchidaceae. Chunjian is one of the representative varieties of orchids, mainly produced in the southwestern region of my country. Chunjian is verdant and green all the year round, its leaves are straight like swords, and its flowers are light and elegant. Based on white, yellow, green, and red colors, the fragrance of flowers is quiet and pleasant; large flowers Cymbidium is a kind of orchid variety selected by hybridization of some large-flowered epiphytic orchids in the genus. Its plant type and flower type are huge, the flower color is bright and colorful, the scape is long, the number of flowers is large, and th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 李春楠傅巧娟沈国正赵福康张晓莹阮若昕
Owner HANGZHOU ACAD OF AGRI SCI
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