A kind of Cymbidium seed aseptic germination method
A seed and sterile water technology, applied in the field of artificial planting of rare and endangered plants, can solve the problems of difficult germination, high cost and low reproduction rate.
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Embodiment 1
[0020] 1. Seed treatment
[0021] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 1.0mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.
[0022] 2. Sowing and cultivating
[0023] Under sterile conditions, mix the treated seeds in step 1 with...
Embodiment 2
[0030] 1. Seed treatment
[0031] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 0.5mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.
[0032] 2. Sowing and cultivating
[0033] Under sterile conditions, mix the treated seeds in step 1 with...
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