Method for Rooting of Aseptic Short Branches of Medicinal Dioscorea scutellaria Tissue Cultured Plantlets

A technology of Dioscorea scutellaria and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of long cultivation period, difficult root differentiation, and low transplanting survival rate of Dioscorea scutellaria, and improve the The effect of transplanting survival rate, solving difficult rooting and simplifying production procedures

Inactive Publication Date: 2017-04-05
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

On the one hand, it is because of the long culture period of Dioscorea scutellaria, and most of the research reports are based on the technical route of explant-callus-callus differentiation into a type of protocorm or bulbils giving birth to a root and a complete plant; inducing callus from explants It takes 120-150 days to form a complete plant after wounding the tissue. The bigger problem is that the conduction system between roots and shoots induced by this method is not connected, and the survival rate of transplanting is lower than 30%. Branches are difficult to take root because of the particularity of the stem structure of Dioscorea scutellaris. There are two circles of vascular bundles in the stem of Dioscorea scutum. The cells are reduced, so it is difficult to have adventitious root differentiation when inducing the rooting of sterile spurs
The above-mentioned problems lead to the inability of the tissue culture method of Dioscorea scutellaria to form a standardized planting, and it is also a bottleneck for the expansion of the market for pharmaceutical products using Dioscorea scutellaria as raw material

Method used

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  • Method for Rooting of Aseptic Short Branches of Medicinal Dioscorea scutellaria Tissue Cultured Plantlets
  • Method for Rooting of Aseptic Short Branches of Medicinal Dioscorea scutellaria Tissue Cultured Plantlets
  • Method for Rooting of Aseptic Short Branches of Medicinal Dioscorea scutellaria Tissue Cultured Plantlets

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1, the method for rapid propagation of Dioscorea scutellaria aseptic short shoot rooting by tissue culture

[0025] 1. Culture medium preparation and sterilization

[0026] Sterile short branch rooting medium: 1 / 2MS large amount + 10*MS trace amount + MS organic + DL iron salt + CCC1.0mg / L + PP 333 0.5mg / L+IAA0.05mg / L+IBA0.01mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.

[0027] 2. Rooting and cultivation

[0028] Take the terminal buds and stems of salvia hispanica L, first wash them with washing powder, wash them 5-7 times with sterile water on a sterile operating table, soak them in 70% alcohol for 1 minute, and then put them in 0.1% mercuric chloride solution for 12 to 18 minutes, rinsed with sterile water for 5 to 6 times, and transferred the terminal buds and axillary buds to the primary culture medium MS+6-BA2.0mg / L+KT0.5mg / L. The number of explants in each flask is 3-5, and the callus and buds are induced aft...

Embodiment 2

[0031] Embodiment 2, the method for rapid propagation of Dioscorea scutellaria aseptic short branch rooting by tissue culture

[0032] Media Preparation and Sterilization

[0033] Sterile short branch rooting medium: or preferred medium: 1 / 2MS large amount + 10*MS trace amount + MS organic + DL iron salt + CCC 0.8mg / L + PP 333 0.3mg / L+IAA0.03mg / L+IBA0.08mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.

[0034] From the test-tube plantlets of subculture, select the even and strong test-tube plantlets of Dioscorea shielbae in growing way, divide into individual plants and inoculate in the above-mentioned rooting medium, the basic operation method is consistent with embodiment 1; effect is not much different from embodiment 1.

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Abstract

The invention relates to a rooting method of a sterile short shoot of a medicinal peltate yam rhizome tissue culture seedling. The rooting method comprises the following steps: a sterile short shoot acquired by peltate yam rhizome through tissue culture is used as an explant, the sterile short shoot is inoculated in a culture medium formed by a large amount of 1 / 2MS, a little amount of 10*MS, organic MS, DL ferric salt, 0.1-2.0mg / L of CCC, 0.1-2.0mg / L of PP333, 0.01-0.1mg / L of IAA, 0.01-0.2mg / L of IBA, saccharose and agar for 25-40d to form a large number of adventive roots, wherein the addition amount of saccharose is 3% and the addition amount of agar is 0.75%; and finally, the adventive root is transplanted to the field for seed stem breeding. According to the rooting method, the production procedure of the health seedling can be simplified, the production cost is low, a problem that the sterile short shoot cannot realize rooting easily is solved and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of medicinal Dioscorea scutellaria, in particular to a method for rooting aseptic short shoots of medicinal Dioscorea scutellaria. Background technique [0002] Dioscorea (Dioscorea) is not only a national second-class protected plant, but the dioscorea contained in its rhizome is the most basic raw material for the synthesis of hormone drugs, and is known as the "mother of hormones". Dioscorea shield leaf is one of the medicinal plants with the highest diosgenin content in my country, and one of the medicinal plants with the highest diosgenin content in China. [0003] At home and abroad, tissue culture technology has been used to propagate yam plants in the mid-1970s, but most of them stay in the stage of laboratory technology research and fail to produce seedlings on a large scale. On the one hand, it is because of the long culture period of Dioscorea scutellaria, and most of the resear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 夏新界黄丽芳刘永源杨平辉
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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