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Cymbidium seed aseptic germination method

A seed and sterile water technology, applied in the field of artificial planting of rare and endangered plants, can solve the problems of small Cymbidium seeds, slow reproduction speed, and low reproduction rate, and achieve the effects of short germination period, low cost, and low reproduction rate

Active Publication Date: 2019-01-04
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of Cymbidium cymbidium in view of the problem that the seeds of Cymbidium are small, have no endosperm, and are extremely difficult to germinate under natural conditions, and the traditional reproduction method of Cymbidium ramets has low reproduction rate, slow propagation speed and high cost. How to quickly germinate orchid seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Seed treatment

[0021] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 1.0mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.

[0022] 2. Sowing and cultivating

[0023] Under sterile conditions, mix the treated seeds in step 1 with...

Embodiment 2

[0030] 1. Seed treatment

[0031] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 0.5mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.

[0032] 2. Sowing and cultivating

[0033] Under sterile conditions, mix the treated seeds in step 1 with...

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PUM

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Abstract

The invention discloses a cymbidium seed aseptic germination method. According to the method, cymbidium seeds are disinfected and then treated by gibberellin GA3; sowing the treated seeds into an aseptic germination culture medium under aseptic conditions to culture the seeds. The method is simple, easy, low in cost, high in seed germination rate and short in germination period, the seed germination rate can reach 30-50%, a lot of cymbidium seedlings can be rapidly acquired, and large-area and large-scale seedling culture requirements can be met.

Description

technical field [0001] The invention belongs to the technical field of artificial planting of rare and endangered plants, and in particular relates to a seed aseptic germination method of the orchid plant Cymbidium. Background technique [0002] Cymbidium faberi (Cymbidium faberi) is a ground herbaceous plant of the genus Orchidaceae, with inconspicuous pseudobulbs. As a large species of Chinese orchids in the traditional sense, it is deeply loved by people for its rich and pure aroma, elegant and graceful flower shape, and elegant and firm plant shape. At present, it is mainly produced in southern Shaanxi, southern Gansu, Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan, Guizhou, Yunnan and other places. Cymbidium has a long history of cultivation, and there are many traditional fine varieties. Some of them have been cultivated for hundreds of years. [0003] Cymbidium grows naturally in hillside forests, and most of the cultivated varieties are selected from the natural v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 牛俊峰刘帅王喆之吴小强王世强胡喜风胡晓瑜张淑柯黄飞
Owner SHAANXI NORMAL UNIV
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