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Production method of culture medium for landscaping

A production method and landscaping technology, applied in the field of landscaping medium production, can solve the problems of not preventing fungal pollution, reducing the success rate of cultivation, and single nutritional structure, so as to promote the growth of callus and accelerate the growth of callus. The effect of plant growth speed and increased survival rate

Inactive Publication Date: 2022-01-28
禾朴生态建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the cultivation of some precious landscaping trees, the method of plugging is usually used for cultivation, and the cultivation efficiency is low, and the success rate is relatively low, especially for the cultivation of the southern yew. The success rate of inoculation is low, and it is usually cultivated in the form of a petri dish. The internal nutritional structure of the existing petri dish is relatively single, and there are no good measures to prevent fungal contamination in the subsequent cultivation, thereby reducing the The overall success rate of cultivation, for this reason, we propose a production method of culture medium for landscaping

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for producing a medium for landscaping, comprising the following raw materials in parts by weight: 1L of distilled water, 0.5mmol of inorganic elements, 8g of glucose, 200mg of vitamins, 8g of agar and 10mg of streptomycin, comprising the following steps:

[0018] S1. Mixing of inorganic elements: take MgSO4 7H20, KCL and NH4-N and add them into water according to 3:1.5:1 and mix them;

[0019] S2. Vitamin mixing: take Vpp niacin and inositol and mix them in water at a ratio of 1:1;

[0020] S3. Heating and mixing: keep warm after removing distilled water, add inorganic elements and vitamins and mix thoroughly, then add agar, and stir fully after the agar is completely melted;

[0021] S4. High-temperature disinfection: After the mixed solution is divided into packages and sealed, put into an autoclave, take out after high-temperature cooking and sterilization, add streptomycin after cooling and put it into a sterile environment, and wait for complete solidific...

Embodiment 2

[0025] A method for producing a medium for landscaping, comprising the following raw materials in parts by weight: 3L of distilled water, 1.5mmol of inorganic elements, 24g of glucose, 600mg of vitamins, 24g of agar and 30mg of streptomycin, comprising the following steps:

[0026] S1. Mixing of inorganic elements: Take MgSO4 7H20, KCL and NH4-N and add them into water according to 3:1.5:1 and mix them;

[0027] S2. Vitamin mixing: take Vpp niacin and inositol and mix them in water at a ratio of 1:1;

[0028] S3. Heating and mixing: keep warm after removing distilled water, add inorganic elements and vitamins and mix thoroughly, then add agar, and stir fully after the agar is completely melted;

[0029] S4. High-temperature disinfection: After the mixed solution is divided into packages and sealed, put into an autoclave, take out after high-temperature cooking and sterilization, add streptomycin after cooling and put it into a sterile environment, and wait for complete solidif...

Embodiment 3

[0033] A method for producing a medium for landscaping, comprising the following raw materials in parts by weight: 2L of distilled water, 1mmol of inorganic elements, 16g of glucose, 400mg of vitamins, 16g of agar and 20mg of streptomycin, comprising the following steps:

[0034] S1. Mixing of inorganic elements: Take MgSO4 7H20, KCL and NH4-N and add them into water according to 3:1.5:1 and mix them;

[0035] S2. Vitamin mixing: take Vpp niacin and inositol and mix them in water at a ratio of 1:1;

[0036] S3. Heating and mixing: keep warm after removing distilled water, add inorganic elements and vitamins and mix thoroughly, then add agar, and stir fully after the agar is completely melted;

[0037] S4. High-temperature disinfection: After the mixed solution is divided into packages and sealed, put into an autoclave, take out after high-temperature cooking and sterilization, add streptomycin after cooling and put it into a sterile environment, and wait for complete solidific...

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Abstract

The invention discloses a production method of a culture medium for landscaping. The culture medium comprises the following raw materials in parts by weight: 1-3 L of distilled water, 0.5-1.5 mmol of inorganic elements, 8-24 g of glucose, 200-600 mg of vitamins, 8-24 g of agar and 10-30 mg of streptomycin. The process is mature, microelements are provided for plant growth through MgSO<4>.7H2O, KCl and NH4-N in the inorganic elements, and through Vpp nicotinic acid in the vitamins, plant metabolism and embryo development are promoted, 100 mg / L of inositol helps mutual transformation of carbohydrates, can promote growth of calluses and formation of embryoids and buds, has a promoting effect on propagation and differentiation of tissues and cells, also has an effect on formation of cell walls and accelerates root development, and the streptomycin prevents bacterial contamination and reduces material loss in culture. The whole method can be widely applied to cuttage grafting culture of taxus chinensis var mairei.

Description

technical field [0001] The invention relates to a plant culture medium production process, and more specifically, relates to a production method of a landscaping culture medium. Background technique [0002] Culture medium is like soil, which is the base for the survival and development of in vitro materials in tissue culture. Therefore, in each link of tissue culture, we should focus on mastering the culture medium, understanding its composition and preparation methods, and the success of tissue culture depends to a large extent on the choice of culture medium. Different media have different characteristics and are suitable for different plant species and inoculum materials. When carrying out tissue culture activities, various media should be understood and analyzed so that they can be selected for use. [0003] For the cultivation of some precious landscaping trees, the method of plugging is usually used for cultivation, and the cultivation efficiency is low, and the suc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 孙迪陈敏徐杰
Owner 禾朴生态建设有限公司
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