Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting 10-DAB from taxus chinensis var mairei

A technology of 10-DAB and Taxus chinensis, applied in the field of extraction of 10-DAB in Taxus chinensis, can solve the problems of low 10-DAB content, unfavorable industrialized production and high cost, reduce the use of organic solvents, and solve production problems. The effect of long cycle and reduction of liquid-material ratio

Pending Publication Date: 2020-10-09
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of 10-DAB in yew has been reported. The general method for extracting 10-DAB from yew includes the following steps: yew→crushing→methanol ultrasonic extraction→combining extracts→chloroform extraction and purification→decompression Concentration → Methanol to constant volume. Due to the lack of research on extraction conditions, the content of 10-DAB extracted from Taxus chinensis is low and the cost is high, which is not conducive to industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting 10-DAB from taxus chinensis var mairei
  • Method for extracting 10-DAB from taxus chinensis var mairei
  • Method for extracting 10-DAB from taxus chinensis var mairei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Extraction of 10-DAB in Taxus chinensis of the present invention

[0022] The southern yew sample was dried, crushed, and passed through a No. 4 sieve. Accurately weigh 20g, add 300ml methanol, and extract by ultrasonic for 23min at a temperature of 40°C, extract three times in total, and centrifuge at a high speed (4000r min -1 ) for 5 minutes, combined the three supernatants, concentrated under reduced pressure for 20 minutes at a temperature of 60° C. and a pressure of 0.08 MPa to 1 g, and obtained the concentrate.

Embodiment 2

[0023] Example 2 Extraction of 10-DAB in Taxus chinensis of the present invention

[0024] The southern yew sample was dried, crushed, and passed through a No. 4 sieve. Accurately weigh 20g, add 100ml of methanol, and conduct ultrasonic extraction for 40min at a temperature of 20°C for a total of three extractions, high-speed centrifugation (4000r min -1 ) for 5 minutes, combined the three supernatants, concentrated under reduced pressure for 20 minutes at a temperature of 60° C. and a pressure of 0.08 MPa to 1 g, and obtained the concentrate.

Embodiment 3

[0025] Example 3 Extraction of 10-DAB in Taxus chinensis of the present invention

[0026] The southern yew sample was dried, crushed, and passed through a No. 4 sieve. Accurately weigh 20g, add 500ml of methanol, and ultrasonically extract for 25min at a temperature of 40°C, extract three times in total, and centrifuge at a high speed (4000r min -1 ) for 5 minutes, combined the three supernatants, concentrated under reduced pressure for 20 minutes at a temperature of 60° C. and a pressure of 0.08 MPa to 1 g, and obtained the concentrate.

[0027] The beneficial effects of the present invention are illustrated below through test examples.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting 10-DAB from taxus chinensis var mairei, which comprises the following steps: taking taxus chinensis var mairei, drying, pulverizing, screening, adding methanol according to a solid-liquid ratio of 1: (5-25) g / ml, carrying out ultrasonic extraction, centrifuging the extracting solution, taking a supernatant, and concentrating to obtain the 10-DAB-containing extract. Compared with the existing extraction technology, the method for extracting the 10-DAB from the taxus chinensis var mairei has the advantages that the liquid-to-solid ratio is reduced,the use of an organic solvent is reduced, the extraction time is shortened, the extraction efficiency is improved, and the problem of long production period of the 10-DAB extraction technology is fundamentally solved. The 10-DAB obtained by the preparation method disclosed by the invention is high in content and has practical promotion and application values.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting 10-DAB from Taxus chinensis. Background technique [0002] Southern yew (Taxus chinensis var.mairei) is one of the most widely distributed and fastest-growing species in the Taxaceae (Taxaceac) genus Taxus. It is a unique natural anti-cancer plant in my country and belongs to the national first-class Protect plants. Paclitaxel is its main anticancer active ingredient. However, paclitaxel is a secondary metabolite, which is mostly obtained from the bark of Taxus plants, and its content is extremely low. And there are disadvantages such as poor water solubility, low oral availability and drug resistance, which limit the safety and effectiveness of clinical medication. Therefore, it is necessary to develop and utilize the remaining active substances in Taxus chinensis to meet market demand. [0003] The full name of 10-DAB is 10-deacetyl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 李玉芝曹治兴裴瑾宋肖敏婷
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com