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Artificial hybridization method of taxus chinensis

An artificial hybridization and yew technology, applied in the field of artificial hybridization of yew, can solve the problems affecting the process of hybridization and breeding of yew plants, difficulty in hybridization, and asynchronous flowering of male and female plants of yew, and achieve good prospects and strong The effect of heterosis

Inactive Publication Date: 2017-11-24
JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Taxus is a dioecious plant. Because the flowering period of male flowers of different species of Taxus is different, and the flowering period of male and female plants of the same species of Taxus is not synchronized, it makes hybridization difficult, which seriously affects the process of hybrid breeding of Taxus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment one: a kind of yew artificial hybridization method comprises the following steps:

[0015] (1) Pollen collection:

[0016] When the female Taxus chinensis plant is in full bloom, collect the male flowers of Taxus chinensis that bloom on the same day;

[0017] (2) Processing method:

[0018] Spread the male flowers thinly on white paper, cover the top with white paper, put them in the sun, and turn them over every 2 hours. After 1 day, you can see the powder, sieve lightly with a sieve to remove the sundries; wash the pollen with clean water first According to the weight ratio of pollen: water = 1:1000 ratio to prepare pollen liquid, then add chitosan, borax and sugar in the pollen liquid, the addition amount is by weight: chitosan 0.1%, borax 3%, sugar 5% %, to ensure the vitality of pollen and improve the success rate of hybridization;

[0019] (3) Spray hybridization with a sprayer. The best hybridization time for the female Taxus chinensis plant is just...

Embodiment 2

[0022] Embodiment two: a kind of yew artificial hybridization method comprises the following steps:

[0023] (1) Pollen collection:

[0024] When the female Taxus chinensis plant is in full bloom, collect the male flowers of Taxus chinensis that bloom on the same day;

[0025] (2) Processing method:

[0026] Spread the male flowers thinly on the white paper, cover the top with white paper, put it in the sun, and turn it over every 2.5 hours. After 1 day, you can see the powder, and use a sieve to remove the sundries; wash the pollen with water first. According to weight ratio pollen: water=1: 2000 ratio is modulated into pollen liquid, then adds chitosan, borax and sugar in pollen liquid, and addition is by weight: chitosan 0.3%, borax 5%, sugar 10% %, to ensure the vitality of pollen and improve the success rate of hybridization;

[0027] (3) Spray hybridization with a sprayer. The best hybridization time for the female Taxus chinensis plant is just after the dew dries in ...

Embodiment 3

[0030] Embodiment three: a kind of yew artificial hybridization method comprises the following steps:

[0031] (1) Pollen collection:

[0032] When the female Taxus chinensis plant is in full bloom, collect the male flowers of Taxus chinensis that bloom on the same day;

[0033] (2) Processing method:

[0034] Spread the male flowers thinly on white paper, cover the top with white paper, put them in the sun, and turn them over every 2 hours. After 1 day, you can see the powder, sieve lightly with a sieve to remove the sundries; wash the pollen with clean water first According to the weight ratio of pollen: water = 1:1500 ratio to prepare pollen liquid, then add chitosan, borax and sugar in the pollen liquid, the addition amount is by weight: chitosan 0.2%, borax 4%, sugar 6 %, to ensure the vitality of pollen and improve the success rate of hybridization;

[0035] (3) Spray hybridization with a sprayer. The best hybridization time for the female Taxus chinensis plant is jus...

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Abstract

The invention relates to an artificial hybridization method of taxus chinensis. The artificial hybridization method is characterized by comprising the following steps: (1) collection of pollen: when taxus chinensis female plant is at a full-bloom stage, collecting taxus chinensis var mairei male flowers flowering on the day; (2) treatment method: thinly spreading the male flowers on white paper, covering with white paper, airing under sunshine, overturning once for every 2 to 2.5h till seeing pollen after only 1 day, and slightly sieving to remove impurities; blending pollen and clear water into pollen liquid according to the weight ratio of pollen to water being 1:(1000-2000), and then adding the following components in percentage by weight into the pollen liquid: 0.1%-0.3% of chitosan, 3%-5% of borax and 5%-10% of sugar; and (3) conducting spray hybridization through a sprayer, namely spraying once for every 3 to 4 days through the sprayer, and totally performing hybridization for three times. The filial generation obtained by adopting the artificial hybridization method of taxus chinensis not only has the rapid growth characteristics of taxus chinensis var mairei, but also has the cold-resistant and drought-resistant advantages of taxus chinensis.

Description

technical field [0001] The invention relates to a yew artificial hybridization method, which belongs to the technical field of plant planting. Background technique [0002] Taxus is a dioecious plant. Because the flowering period of male flowers of different species of Taxus is different, and the flowering period of male and female plants of the same species of Taxus is not synchronized, it makes hybridization difficult, which seriously affects the process of hybrid breeding of Taxus. . [0003] Hybrid breeding is of great significance to the development and popularization of Taxus chinensis. Contents of the invention [0004] The purpose of the present invention is to overcome the deficiencies in the prior art and to provide a method for artificial hybridization of yew. The hybrid offspring not only has the characteristics of rapid growth of southern yew, but also has the advantages of cold resistance and drought resistance of Mandia yew. It has strong heterosis and has...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 朱波风
Owner JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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