Method for extracting and purifying two kinds of taxane compound from yew branches and leaves

Abstract

The invention relates to a method for using specific resin and silicon gel column chromatography purification via negative pressure cavitation water extraction to high-efficiency produce 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel, which uses taxus branch leaves as raw material, dries and breaks to process negative pressure cavitation water extraction, filters and uses AB-8 resin to process dynamic absorption on filter liquor to concentrate 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel and process further purification via middle pressure silicon gel column chromatography, to obtain the 10-deacetylbaccatin III of at least 60% purity and 7-xylose-10-deacetylpaclitaxel of at least 67% purity. The invention can use renewable taxus branch leaves to protect ecological resource and effectively extract effective component and biological semi synthesis precursor materials, with simple operation, support for industrial application and important industrial production value.

Description

technical field [0001] The invention relates to a method for extracting and purifying 10-deacetylbaccatin III and 7-xylosyl-10-deacetyl paclitaxel from branches and leaves of Taxus chinensis. Background technique [0002] Taxol (paclitaxel, Figure 1) is a diterpenoid compound with a pentamethylpentadecene skeleton, which is a natural anticancer drug extracted from the bark of Pacific yew (Taxus brevifolia) for the first time (Wani et al., 1971), can bind to tubulin, promote its polymerization, inhibit cancer cell mitosis, and prevent cancer cell proliferation (Kelling et al., 2003; She Kun et al., 2001) was officially approved by the US FDA in 1992 as a new anticancer drug , Paclitaxel has a good effect on ovarian cancer, breast cancer, and lung cancer. Studies in recent years have shown that paclitaxel also has certain potential in the treatment of other diseases, such as malignant melanoma (Roshid et al., 1994), rheumatoid arthritis (Oliver et al., 1994) and Alzheimer's d...

AI Technical Summary

Problems solved by technology

Taxanes with xylosyl attached at the C-7 position were originally isolated by Senilh, mainly including: 7-xylosyl-10-deacetyl paclitaxel, 7-xylosyl-10-deacetyl tri Pimemannine, 7-xylosyl-10-deacetyl paclitaxel C, etc. These compounds are often discarded as waste in the traditional production process of extracting paclitaxel, which not only wastes plant resources, but also pollutes the environment

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