Method for extracting and purifying two kinds of taxane compound from yew branches and leaves

A technology of yew branches and leaves and acetyl-paclitaxel, which is applied in organic chemistry and other fields, can solve the problems of wasting plant resources and polluting the environment, and achieve high-efficiency utilization, short time and high efficiency

Inactive Publication Date: 2008-08-06
NORTHEAST FORESTRY UNIVERSITY +1
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Problems solved by technology

Taxanes with xylosyl attached at the C-7 position were originally isolated by Senilh, mainly including: 7-xylosyl-10-deacetyl paclitaxel, 7-xylosyl-10-deacetyl tri Pimemannine, 7-xylosyl-10-deacetyl

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  • Method for extracting and purifying two kinds of taxane compound from yew branches and leaves

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Embodiment 1

[0020] Weigh 200g of southern yew branches and leaves, dry and pulverize, add 2000mL of water for negative pressure cavitation extraction, filter after 30min, and add the filtrate to a glass column equipped with 35g of AB-8 resin for dynamic adsorption. The loading solution is 15BV, The concentrations of 10-deacetylbaccatin III and 7-xylosyl-10-deacetylpaclitaxel in the sample solution were: 0.0657mg / mL and 0.1681mg / mL, the flow rate was 1mL / min, and the temperature was 35 ℃, using gradient elution method: 3BV of 30% ethanol was eluted to remove impurities, and then 6BV was eluted with 80% ethanol, the fractions were collected and concentrated to dryness. The content of -xylosyl-10-deacetyl paclitaxel was increased from 0.2% and 0.053% to 1.69% and 3.34%, respectively, which was increased by 8.54 times and 62.43 times respectively. After the resin was enriched, the sample was purified by 300-400 mesh medium-pressure silica gel column chromatography. The silica gel used was 40 ...

Embodiment 2

[0022]Weigh 200g of Chinese yew branches and leaves, dry and pulverize them, add 2000mL of water for negative pressure cavitation extraction, filter after 30min, add the filtrate to a glass column equipped with 35g of AB-8 resin for dynamic adsorption, the loading solution is 15BV, The contents of 10-deacetylbaccatin III and 7-xylosyl-10-deacetylpaclitaxel in the loading solution were 0.0519mg / mL and 0.1673mg / mL respectively, the flow rate was 1mL / min, and the temperature was 35 ℃, using gradient elution method: 3BV of 30% ethanol was eluted to remove impurities, and then 6BV was eluted with 80% ethanol, the fractions were collected and concentrated to dryness. The content of -xylosyl-10-deacetyl paclitaxel increased from 0.16% and 0.049% to 1.57% and 3.46%, respectively, increasing 9.81 times and 70.61 times respectively. After the resin was enriched, the sample was purified by 300-400 mesh medium-pressure silica gel column chromatography. The silica gel used was 40 g, the co...

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Abstract

The invention relates to a method for using specific resin and silicon gel column chromatography purification via negative pressure cavitation water extraction to high-efficiency produce 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel, which uses taxus branch leaves as raw material, dries and breaks to process negative pressure cavitation water extraction, filters and uses AB-8 resin to process dynamic absorption on filter liquor to concentrate 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel and process further purification via middle pressure silicon gel column chromatography, to obtain the 10-deacetylbaccatin III of at least 60% purity and 7-xylose-10-deacetylpaclitaxel of at least 67% purity. The invention can use renewable taxus branch leaves to protect ecological resource and effectively extract effective component and biological semi synthesis precursor materials, with simple operation, support for industrial application and important industrial production value.

Description

technical field [0001] The invention relates to a method for extracting and purifying 10-deacetylbaccatin III and 7-xylosyl-10-deacetyl paclitaxel from branches and leaves of Taxus chinensis. Background technique [0002] Taxol (paclitaxel, Figure 1) is a diterpenoid compound with a pentamethylpentadecene skeleton, which is a natural anticancer drug extracted from the bark of Pacific yew (Taxus brevifolia) for the first time (Wani et al., 1971), can bind to tubulin, promote its polymerization, inhibit cancer cell mitosis, and prevent cancer cell proliferation (Kelling et al., 2003; She Kun et al., 2001) was officially approved by the US FDA in 1992 as a new anticancer drug , Paclitaxel has a good effect on ovarian cancer, breast cancer, and lung cancer. Studies in recent years have shown that paclitaxel also has certain potential in the treatment of other diseases, such as malignant melanoma (Roshid et al., 1994), rheumatoid arthritis (Oliver et al., 1994) and Alzheimer's d...

Claims

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Application Information

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IPC IPC(8): C07D305/14
Inventor 付玉杰祖元刚孙蕊李双明王莹姜守刚汪琢张琳刘威
Owner NORTHEAST FORESTRY UNIVERSITY
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