Method for stopping Taxus chinensis var. mairei seed dormancy

A southern yew, seed technology, applied in the direction of seed and rhizome treatment, application, agriculture, etc., can solve the problems of time-consuming, laborious, seed embryo damage, etc., and achieve the effect of ensuring the quality of seeds and improving the utilization rate

Active Publication Date: 2013-07-17
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Although this method effectively improves the germination rate, it needs to peel off the outer seed coat of the seed. Obviously, the operation operation will

Method used

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  • Method for stopping Taxus chinensis var. mairei seed dormancy
  • Method for stopping Taxus chinensis var. mairei seed dormancy
  • Method for stopping Taxus chinensis var. mairei seed dormancy

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Embodiment 1（ comparative Embodiment ）

[0033] The reagents used in this embodiment are as follows: GA 3 : Analytical grade, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110418; 6-benzylaminoadenine (6-BA): analytical grade, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110505; Aiduoshou: analytical grade, Asahi Chemical Co., Ltd., batch number: 20110701; concentrated sulfuric acid: analytically pure, Hangzhou Gaojing Fine Chemical Co., Ltd., batch number: 20101009; NaClO: analytically pure, Hangzhou Gaojing Fine Chemical Co., Ltd., batch number: 20101230; absolute ethanol: analytically pure , Jinhua Xianke Chemical Factory, batch number: 110404.

[0034] ①Seed selection and processing

[0035] Select plump southern yew seeds, soak them in concentrated sulfuric acid with a mass concentration of 60% for 3 hours, wash them with running water for 24 hours, and prepare 0, 50, 200, and 400 mg·L -1 GA3 solution, 0, 5, 10, 20mg·L -1 For 6-BA solution, dilute the Aidoshen so...

Embodiment 2

[0053] Embodiment 2 (comprising comparative example):

[0054] The reagents used in this embodiment are as follows: GA 3 : analytically pure, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110418; 6-BA: analytically pure, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110505; vitamin D: analytically pure, Shanghai Blue Season Technology Development Co., Ltd., batch number : 110623; vitamin E: analytically pure, Shanghai Lanji Technology Development Co., Ltd., batch number: 110720; indole butyric acid: analytically pure, Shanghai Lanji Technology Development Co., Ltd., batch number: 110810; paclobutrazol: analytically pure, Shanghai Lanji Technology Development Co., Ltd., batch number: 20111015; L-sodium glutamate: analytically pure, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110328; glycine: analytically pure, Shanghai Blue Season Technology Development Co., Ltd., batch number: 110915; citric acid: Analytic...

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Abstract

The invention belongs to the technical field of plant budding, particularly relates to a method for stopping Taxus chinensis var. mairei seed dormancy, and solves the technical problem of slow budding, low budding rate and the like. The method includes the steps of A, soaking Taxus chinensis var. mairei seeds in sulfuric acid 55-65% in mass concentration for 2-4 hours, and washing the soaked seeds with flow water for 12-36 hours; B, soaking the acid etched seeds in pre-prepared solution for at least 3 hours, and soaking the soaked seeds in clean water for at least 3 hours; and C, performing low-temperature stratification or variable-temperature stratification to the seeds soaked by clean water. The method has the advantages that the method is time-saving, effective and economical, the problems that dormancy is deep, budding is difficult and budding rate is low are solved, standards are provided for seed seedling market circulation, seed quality is guaranteed, and great significance is brought to breeding cultivation development of Taxus chinensis var. mairei.

Description

technical field [0001] The invention belongs to the technical field of plant seed germination, and in particular relates to a method for breaking the dormancy of the southern yew seeds. Background technique [0002] Southern yew is a plant of the genus Taxus in the Taxaceae family. It is widely distributed in Europe, North America, East Asia and other northern hemisphere regions. In my country, it is mainly distributed in the Yangtze River Basin and its southern provinces. Modern studies have shown that it mainly contains taxane components such as paclitaxel and 10-deacetylbaccatin III, as well as a small amount of flavonoids and polysaccharides. Among them, paclitaxel, an anticancer active ingredient, can induce the apoptosis of cancer cells by inhibiting the depolymerization of tumor cell microtubules. Because of its unique anticancer mechanism and significant curative effect, it is used to treat lung cancer, breast cancer, ovarian cancer and esophageal cancer and other m...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 张春椿熊耀康俞冰张水利李效贤蒋剑平
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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