Method for extracting coronatine

A technology for coronatine and microbial fermentation broth, applied in the field of coronatine extraction, can solve the problems of large consumption of organic solvents, difficult removal of impurities, easy environmental pollution, etc., and achieves the effects of low cost, good selectivity, and large adsorption capacity

Active Publication Date: 2007-10-17
CHENGDU NEWSUN CROPSCI
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In above-mentioned preparation technique, there are following shortcoming: (1) consume a large amount of organic solvents, cost height (2) operation is loaded down with trivial details (3) easily pollute environment, (4) shortcoming (Uppalapati S R , Ayoubi P, Weng H, Palmer D A, Mitchell R E, Jones W and Bender C L. The phytotoxin coronatine and methyl jasmonate impact multiple phytohormone pathways in tomato. Plant. J. 2005, 42, 201-217.)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, extracting coronatin and its effect verification

[0024] Pick the high-temperature-resistant mutant of Pseudomonas syringae soybean pathogenic variety MFB141 (the MFB141 strain has been preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), the preservation number is CGMCC1276, and the Chinese patent number is ZL 200510011466.2). In slant medium (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl 0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; solid medium: add 1.3% agar powder, sterilize at 121°C for 30min), cultivate at 28°C for 48-60h to activate, then pick a ring of activated bacteria, insert The liquid culture medium of the kanamycin of 10ug / ml and the streptomycin of 30ug / ml (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; sterilized at 121°C for 30min), cultivated at 28°C for 36-48h, and when it reach...

Embodiment 2

[0026] Example 2, extracting coronatin and its effect verification

[0027] Pick the high-temperature-resistant mutant of Pseudomonas syringae soybean pathogenic variety MFB141 (the MFB141 strain has been preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), the preservation number is CGMCC1276, and the Chinese patent number is ZL 200510011466.2). In slant medium (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl 0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; solid medium: add 1.3% agar powder, sterilize at 121°C for 30min), cultivate at 28°C for 48-60h to activate, then pick a ring of activated bacteria, insert 10ug / ml of kanamycin and 30ug / ml of streptomycin (please provide the name and concentration of antibiotics added) liquid medium (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl 0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; sterilized at 121°C for 30min), culti...

Embodiment 3

[0029] Example 3, Extraction of coronatin and verification of its effect

[0030] The low-temperature high-yield mutant M-18 of Pseudomonas syringae pathogenic strain of soybean picked and preserved in cryopreservation (the M-18 strain has been preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC), the preservation number is CGMCC1412, and the Chinese patent Application number: 200510085280.1) in slant medium (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl 0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; solid medium: add 1.3% agar powder, sterilize at 121°C for 30min), cultivate at 28°C for 48-60h to activate, then pick a ring of activated bacteria, insert The liquid culture medium of the kanamycin of 10ug / ml and the streptomycin of 30ug / ml (containing mannitol 1%, L-sodium glutamate 0.2%, KH 2 PO 4 0.05%, NaCl0.02%, MgSO 4 ·7H 2 O 0.02%, pH 7.0; sterilized at 121°C for 30min), culti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a method for extracting coronatine, in which coronatine is generated through eluting and separating supernatant of microorganism fermentation liquids containing coronatine with adsorption column that takes macroporous adsorption resin as adsorbing agent. The method of present invention has the features of high extraction yield for coronatine, simple operation, low production cost and low environment pollution.

Description

technical field [0001] The invention relates to a method for extracting coronatine. Background technique [0002] Coronatine (COR) is a non-host specialized toxin produced by several pathogenic strains of the plant pathogen Pseudomonas syringae. (Bender C L, Alarco..n-Chaidez F and Gross DC. Pseudomonas syringae phytotoxins: mode of action, regulation and biosynthesis by peptide and polyketide synthetases. Microbiol. Mol. Biol. Rev. 1999, 63, 266-292; Palmer D A, and C L Bender. Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. Glycinea. Appl. Environ. Microbiol. 1993, 59, 1619-1626.) coronatine is a ring of methyl jasmonate (MeJA) Pentane structural analogs have multiple physiological functions, especially in stress resistance, and can be used as a new type of plant growth regulator. (Uppalapati S R, Ayoubi P, Weng H, Palmer D A, Mitchell R E, Jones W and Bender C L. Thephytotoxin coronatine ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C235/82C07C231/24
Inventor 李召虎章家长段留生张明才吴慧玲焦睿
Owner CHENGDU NEWSUN CROPSCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap