Method for extracting coronatine from fermentation liquor by using membrane separation technique

Abstract

The invention discloses a method for extracting coronatine from fermentation liquor by using a membrane separation technique, comprising micro-filtration and hyper-filtration steps: filtering a fermentation liquor containing coronatine by a micro filtering membrane with the aperture of 0.1-10 Mum so as to remove impurity particles and thalli; then filtering the liquor by a hyper-filtration membrane with the aperture of 0.001-0.1 Mum so as to remove macromolecule impurities of proteins, nucleic acids and colloid granules to obtain clear filtrate; and then carrying out common resin exchange or column chromatography, concentrating and crystallizing to obtain the coronatine. In order to improve the effect of separating purification, pre-treatment can be added before the micro-filtration, namely, the coronatine fermentation liquor is filtered though the filtration membrane with the aperture of 1-10 Mum; nano-filtration can be added after the hyper-filtration, namely, the effluence liquor isfiltered by a nano-membrane system with cut-off molecular weight of 400-800. The method in the invention has simple operation and high yield which can be over 90%; in addition, the method has no influence on the structure and activity of the coronatine; and has low energy consumption and low pollution, thus completely satisfying the requirements of industrialized production.

Description

technical field

[0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for separating and extracting active components from fermented liquid by using membrane separation technology. Background technique

[0002] Coronatine (I), also known as coronatine (COR for short), is a physiologically active substance produced by microorganisms discovered in the late 1970s. It consists of two special structural parts, namely bicyclic carboxylic acids, called crowns. Acid (II) (coronafacic acid, referred to as CFA), and cyclopropyl amino acid, known as crown amino acid (III) (coronamicacid, referred to as CMA).

[0003] Coronatins exhibit multiple functions in plant growth regulation, such as promoting tuber formation and expansion, inhibiting seed germination and root growth, and promoting fruit abscission, etc. (Weiler, 1994; Vignutelli, 1998; Koda, 1994). A number of patents have been applied for the application of coronatin. For exa...

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