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Drought-resistant agent and application thereof

A kind of technology of drought resistance agent and formulation, applied in the field of drought resistance agent

Active Publication Date: 2012-01-25
CHENGDU NEWSUN CROPSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of water resources affects the growth and development of crops, resulting in reduced yields, and even crop failure in severe cases

Method used

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  • Drought-resistant agent and application thereof
  • Drought-resistant agent and application thereof
  • Drought-resistant agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, preparation coronatin

[0058] The standard product of coronatin (COR) was purchased from Sigma-Aldrich Company, the content was >95%, and the product number was C8115-1MG.

[0059] The recombinant strain Pseudomonas syringae pathogenic strain MW123 of soybean (purchased from CGMCC, preservation number CGMCC No.2533) was inoculated into a culture medium containing kanamycin with a final concentration of 10 μg / L and streptomycin with a final concentration of 30 μg / L. In MG liquid culture medium, cultured on a shaker at a temperature of 28° C. and a rotation speed of 260 rpm for 48 hours to obtain a primary seed liquid.

[0060] The composition of MG liquid medium is as follows:

[0061]

[0062]

[0063] Solid medium: add 1.5% agar powder

[0064] Sterilize at 121°C for 20 minutes

[0065] The composition of HSC medium is as follows:

[0066] Liquid A:

[0067]

[0068] Liquid B:

[0069] Glucose 20g

[0070] Distilled water 10mL

[0071] L...

Embodiment 2

[0077] Embodiment 2, the drought resistance test on wheat seed germination

[0078] The wheat variety is Shan 253, which is relatively sensitive to moisture. Selected uniform seeds were soaked in 10% hydrogen peroxide solution for 10 minutes, rinsed with distilled water for 5 times, then soaked in distilled water for 12 hours, and transferred to 3 In a plastic box with a cover (12 × 15 cm) with a layer of filter paper, 100 grains per box, placed in a 20-degree artificial climate box for a germination test under dark conditions, each treatment was repeated 4 times, and the seeds of the drought blank control were not treated with pesticides. Soaking treatment; water blank control without PEG-6000 and drug treatment. The germination potential and germination rate were counted on the 4th day and the 7th day after the treatment started; 25 seedlings were randomly selected after 7 days to measure the seedling height, fresh weight per plant, and dry weight per plant; 10 seedlings we...

Embodiment 3

[0083] Embodiment 3, the drought resistance test of wheat field production

[0084] The material is wheat, and the variety is Jingdong 8. The test was carried out in 2009 at the experimental base of China Agricultural University in Shangzhuang Township, Haidian District, Beijing, and the soil was loam. Irrigation during the growth period mainly adopts the method of irrigation with hoses to the field, the irrigation volume is 900 cubic meters per hectare, and three drought methods are set: "no watering", "winter watering" and "winter watering + grouting water" , repeated 4 times, and investigated the yield of the plot when harvesting. Set up single-use medicine and water blank control. The area of ​​the plot is 30 square meters, and the treatment is carried out by uniform spraying on the leaves. See Table 3 for the treatment and concentration of the chemicals.

[0085] "No watering" group: the first chemical treatment was carried out at the rejuvenation stage, and the amount...

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Abstract

The invention discloses a drought-resistant agent and application thereof. The plant drought-resistant agent comprises the active ingredients such as coronatine (COR) and S-abscisic acid (S-ABA). Compared with the present substance for improving the drought resistance of crops, the drought-resistant agent has the following characteristics: unexpected synergy can be obtained through COR, S-ABA and other plant growth adjusting substances which are added on the basis of the COR and S-ABA; an expected addition effect on the growth of crops and the adjustment of the activities can be exceeded, and on one hand, the crop application range can be enlarged, and on the other hand, the application concentration of coronatine, S-ABA and other plant growth adjusting agents can be reduced; and moreover, under the condition that the concentration of a single compound is lower than the effective concentration, a good adjusting degree on the growth of plants can be obtained through the plant growth adjusting agent.

Description

technical field [0001] The present invention relates to a kind of anti-drought agent and its application. Background technique [0002] In recent years, with global warming, large-scale droughts have occurred frequently around the world, and water resource problems have become increasingly prominent. The tense situation of water resources in my country is particularly severe. According to statistics, my country's per capita fresh water resources are less than 1 / 4 of the world's average level, and the temporal and spatial distribution of water resources is extremely unbalanced. , water resources only account for 9.7% of the country (Jiang Heping, China Development Watch, 2009: 28-30). The lack of water resources affects the growth and development of crops, leading to reduced yields, and even to the failure of crops in severe cases. According to reports, the crop loss caused by drought is equivalent to the comprehensive loss caused by other natural disasters (Ding Shengjun, C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/46A01N37/42A01P21/00A01N61/00A01N43/16A01N43/22A01N43/40A01N43/653A01N57/20
CPCY02A40/10
Inventor 段留生谭伟明吴慧玲高飞李召虎田晓莉张明才
Owner CHENGDU NEWSUN CROPSCI
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