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Coronatine aqueous solution and preparation method thereof

A technology of coronatin and water preparation, which is applied in the field of coronatin water preparation and its preparation, and can solve the problems of no reports of coronatin preparations, etc.

Active Publication Date: 2013-11-20
CHENGDU NEWSUN CROPSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no reports on coronatine preparations

Method used

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  • Coronatine aqueous solution and preparation method thereof
  • Coronatine aqueous solution and preparation method thereof
  • Coronatine aqueous solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the preparation of coronatin

[0038] The non-polar macroporous adsorption resin HPD300 used in the examples was purchased from Cangzhou Baoen Adsorption Material Co., Ltd.

[0039] Silica gel (model ZCX.II, particle size 100-200 mesh) was purchased from Qingdao Ocean Chemical Factory.

[0040] The method for detecting the content of coronatin COR by HPLC is as follows:

[0041] The purified sample was dried and dissolved in chromatographic grade methanol, and then detected by HPLC.

[0042] The HPLC conditions in this example are as follows: the chromatographic column is a 150×4.60mm C8 chromatographic column of Kromasil 5 μm 100A, the mobile phase is 80% methanol solution; the detection wavelength is 208nm, the flow rate is 2mL / min, the injection volume is 20 μL, the standard The retention time corresponding to the peak position of the product is 10min.

[0043] 1. Fermentation

[0044] Pseudomonas syringae pathogenic variety MW123 of soybean with th...

Embodiment 2、01

[0060] Embodiment 2, the preparation of 0.1% coronatin aqueous solution

[0061] 1. Selection and ratio of original drug and auxiliary agent

[0062] 0.11 parts by mass of coronatine with a purity of 95% prepared in Example 1 (that is, about 0.1 parts by mass of coronatine);

[0063] Methanol (used as solvent) 5 mass parts;

[0064] Surfactant: 5 parts by mass;

[0065] Preservative: 1 part by mass of Kason;

[0066] Antifreeze: 10 parts by mass of ethylene glycol;

[0067] Defoamer: TSA 775 0.1 parts by mass;

[0068] The remainder is water;

[0069] The total mass of all the above components is 100 parts by mass.

[0070] Two, the preparation of 0.1% coronatin aqueous solution

[0071] Dissolve coronatine with methanol and part of water to obtain coronatin mother liquor; add remaining water to the stirring tank, then add coronatin mother liquor, stir at 2000rpm for 30 minutes, then add antifreeze, surfactant and preservative in turn And antifoaming agent, contin...

Embodiment 3

[0074] Embodiment 3, the preparation of 0.003% coronatin aqueous solution

[0075] 1. Selection and ratio of original drug and auxiliary agent

[0076] 0.0032 parts by mass of coronatine with a purity of 95% prepared in Example 1 (that is, about 0.003 parts by mass of coronatine);

[0077] Methanol (used as solvent) 1 mass part;

[0078] Surfactant: 2 parts by mass of alkyl glycoside APG1200;

[0079] Preservative: 0.5 parts by mass of sodium benzoate;

[0080] Antifreeze: 5 parts by mass of ethylene glycol;

[0081] Defoamer: TSA 775 0.5 parts by mass;

[0082] The remainder is water;

[0083] The total mass of all the above components is 100 parts by mass.

[0084] Two, the preparation of 0.003% coronatin aqueous solution

[0085] Dissolve coronatine with methanol and part of water to obtain coronatin mother liquor; add remaining water to the stirring tank, then add coronatin mother liquor, stir at 3000rpm for 20 minutes, then add antifreeze, surfactant and preservati...

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Abstract

The invention discloses a coronatine aqueous solution and a preparation method thereof. The coronatine aqueous solution having functions of a plant-growth regulator and comprises coronatine, a surfactant, an antifreezing agent, an antifoaming agent, an antiseptic and water. The coronatine aqueous solution also comprises an organic solvent. 100 mass parts of the coronatine aqueous solution comprises 0.0001-0.1 mass parts of coronatine, 0.5-5 mass parts of the surfactant, 2-10 mass parts of the antifreezing agent, 0.1-1 mass part of the antifoaming agent, 0.1-1 mass part of the antiseptic, 0.1-5 mass parts of an organic solvent and the balance water. The coronatine aqueous solution has reasonable coronatine content, adopts a small amount of the organic solvent, does not product dust drifting, does not burn and explode easily, has high biological activity, is safe and environmentally friendly, effectively improves a plant object yield and improves quality.

Description

technical field [0001] The invention relates to an aqueous solution of coronatin and a preparation method thereof. Background technique [0002] Coronatine is a non-host transforming toxin with a methyl jasmonate (Methyl Jasmonate) cyclopentane structure produced by several pathogenic strains of the plant pathogen Pseudomonas syringae. Function, especially in stress resistance, can be used as a new type of plant growth regulator. [0003] Existing studies have reported that coronatine has a growth-promoting effect on rice, corn, wheat, cucumber, tomato, sweet pepper, cauliflower, cabbage and other crops grown under adverse conditions such as high temperature, cold damage, salt damage, and drought, and can significantly improve Tolerance of plant adversity stress. [0004] The current fermentation production yield of coronatin is too low, and the preparation cost of high content of the original drug is high, which is not convenient for dosage form processing. As a plant st...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01N25/02A01P21/00
Inventor 谭伟明段留生李召虎于春欣高飞
Owner CHENGDU NEWSUN CROPSCI
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