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Microbial source combined biostimulant and application thereof

A technology of biostimulants and microbial sources, applied in the field of agricultural applications, can solve the problems of inability to increase production, disease resistance, and stress resistance, and achieve the effects of improving disease resistance and stress resistance, improving disease resistance, and improving crop quality

Inactive Publication Date: 2020-10-09
轩凯生物科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The internal cause of crop production increase is the crop itself, and the external cause is fertilizer. If one aspect is emphasized alone, the goals of maximum yield increase, disease resistance, and stress resistance cannot be achieved.

Method used

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  • Microbial source combined biostimulant and application thereof
  • Microbial source combined biostimulant and application thereof
  • Microbial source combined biostimulant and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0025] Preparation of polyglutamic acid: polyglutamic acid fermentation broth after fermentation (refer to literature Xu Z, Feng X, DanZ, et, al.Enhanced poly (γ-glutamic acid) fermentation by Bacillus subtilis, NX-2 immobilized in Add concentrated sulfuric acid to aerobic plant fibrous-bed bioreactor[J].Bioresour Technol,2014,155:8-14) to adjust the pH to about 3.5, raise the temperature to 80°C, keep warm and stir for 1 hour, then adjust the pH with sodium hydroxide to 7.5, that is, the polyglutamic acid whose molecular weight is 5-100kDa according to the present invention is prepared;

[0026] Preparation of amino oligosaccharins: crude enzyme preparation prepared by Escherichia coli fermentation was added to 50 g / L chitosan solution at a ratio of 1:100 (v crude enzyme preparation / v substrate volume), and reacted at 50°C for 8 hours. After the reaction mixture is deactivated, it becomes the concentrated solution of amino oligosaccharides, that is, the concentrated solution ...

Embodiment 1

[0030] Example 1 A microbial source combined biostimulant and its growth-promoting effect on rice seedlings.

[0031] The combined biostimulants include the following three combinations:

[0032] Combination 1: Mix polyglutamic acid, amino oligosaccharin, microbial polysaccharide, and water to prepare a combined biostimulant. Wherein, the polyglutamic acid concentration is 10g / L, the amino oligosaccharin concentration is 8g / L, and the microbial polysaccharide concentration is 15g / L.

[0033] Combination 2: Mix polyglutamic acid, γ-aminobutyric acid, and water to prepare a combined biostimulant. Wherein, the concentration of polyglutamic acid is 10 g / L, and the concentration of γ-aminobutyric acid is 13 g / L.

[0034] Combination 3: Mix microbial polysaccharides, amino oligosaccharins, γ-aminobutyric acid and water to prepare a combined biostimulant. Wherein, the concentration of microbial polysaccharide is 5g / L, the concentration of oligosaccharin is 8g / L, and the concentrat...

Embodiment 2

[0046] Example 2 Combination biostimulants derived from microorganisms and their effects on rice salt tolerance.

[0047] The combined biostimulants include the following three combinations:

[0048] Combination 4: Mix polyglutamic acid, pantoea and water to prepare a combined biostimulant. Wherein, the polyglutamic acid concentration is 20g / L, and the pantoea polysaccharide concentration is 10g / L.

[0049] Combination 5: Mix pantoea, γ-aminobutyric acid, and water to prepare a combined biostimulant. Wherein, the concentration of pantoea polysaccharide is 10g / L, and the concentration of γ-aminobutyric acid is 13g / L.

[0050] Combination 6: Mix polyglutamic acid, γ-aminobutyric acid, and water to prepare a combined biostimulant. Wherein, the concentration of polyglutamic acid is 10 g / L, and the concentration of γ-aminobutyric acid is 13 g / L.

[0051] In order to investigate the impact of the combined plant biostimulant of the present invention on the salt tolerance of rice,...

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Abstract

The invention discloses a microbial source combined biostimulant. The microbial source combined biostimulant is characterized by being prepared from the following components, by mass: 0.1 to 17.5 percent of polyglutamic acid, 0 to 5.0 percent of amino-oligosaccharin, 0.05 to 2.0 percent of pantoea polysaccharide, 0 to 1.5 percent of gamma-aminobutyric acid and the balance of auxiliary materials. The microbial source combined biostimulant can effectively improve the utilization rate of a plant fertilizer, improve the disease resistance and stress resistance of the plant fertilizer and induce aself-defense system of the plant fertilizer, can be completely biodegraded in soil, and is non-toxic, harmless and pollution-free to plants and the soil environment.

Description

technical field [0001] The invention belongs to the field of agricultural applications, and in particular relates to a combination biostimulant derived from microorganisms and its application. Background technique [0002] my country is a large agricultural country, and cultivated land is the foundation of agricultural development. Excessive use and overdraft of soil power are the current situation of my country's land environment. At the same time, although the extensive use of chemical fertilizers and pesticides has made outstanding contributions to agricultural development and food production, it has also brought many negative problems. The low utilization rate of fertilizers, soil compaction, soil-borne diseases, salinization, water pollution and other phenomena have largely threatened the sustainable development of agriculture. "Achieving zero growth in the use of chemical fertilizers and pesticides by 2020" has entered the action period, the development of green preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/60C05G3/70C05G3/80A01G13/00
CPCA01G13/00C05C11/00C05G3/00C05G3/60C05G3/70C05G3/80C05F11/00
Inventor 周哲詹伊婧殷文锋
Owner 轩凯生物科技(山东)有限公司
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