Endophytic bacteria capable of producing ACCD, its soil renovation bacterium agent and its application

A plant endophytic bacteria and bioremediation technology, applied in the fields of agriculture and environmental pollution control, can solve the problems of small biomass, slow growth of hyperaccumulating plants, affecting the efficiency of phytoremediation, etc., to improve the restoration efficiency and improve plant stress resistance. Sex, the effect of promoting plant growth

Inactive Publication Date: 2012-03-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in the implementation process, such as the low bioavailability of heavy metals in soil, which is not conducive to the absorption and removal of heavy metals by plants; hyperaccumulative plants usually grow slowly and have small biomass, etc. These shortcomings seriously affect phytoremediation. s efficiency

Method used

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  • Endophytic bacteria capable of producing ACCD, its soil renovation bacterium agent and its application
  • Endophytic bacteria capable of producing ACCD, its soil renovation bacterium agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The plant endophytic bacteria Jp3-3 bacterial strain of high-yield ACCD in the present invention is obtained from the separation and purification of the stem of Comnyza canadensis growing in a certain copper tailing area in Jiangxi Province, China, and the separation method is as follows:

[0027] First clean the surface of the stems of the small fly with tap water, then wash it again with sterile water, then soak it in 75% ethanol for 5 minutes, rinse it with sterile water, then immerse it in 2.5% sodium hypochlorite solution for 5 minutes, rinse it several times with sterile water . Put the surface-sterilized stems in a mortar and grind until homogenized, take 0.1ml of the homogenate and spread it on an LB plate (10g of peptone, 5g of yeast extract, 10g of NaCl, 1000mL of water, 20g of agar, pH 7.0), and incubate at 28°C for 3 days , Pick a single colony, streak it on an LB plate and save it after purification. The strains were inoculated in LB culture medium, shaken...

Embodiment 2

[0029] Streak inoculation of Jp3-3 (CCTCC NO: M 2011250) in LB medium containing different concentrations of heavy metals, culture at 30°C for 3 days, and observe its growth and growth conditions. The results are shown in Table 1. Jp3-3 strain can tolerate a variety of heavy metals (minimum inhibitory concentrations of Cu, Pb, Cd, Ni are 3.1, 4.8, 4.5, 0.9mM, respectively).

[0030] Table 1 The growth of bacterial strains on LB medium containing different concentrations of heavy metals

[0031]

[0032] In the above table, "+" indicates good growth, and "-" indicates no growth.

Embodiment 3

[0034] Jp3-3 (CCTCC NO: M 2011250) was inoculated in LB culture medium and cultured on a shaking table for 20 h, collected by centrifugation at 4°C, and was mixed with SM culture medium (glucose 1.0 g, sucrose 1.0 g, sodium citrate 1.0 g, apple Acid 1.0g, Mannitol 1.0g, Sodium Acetate 1.0g, KH 2 PO 4 0.4g, K 2 HPO 4 2.0g, MgSO 4 0.2g, CaCl 2 0.1g, CuSO 4 1mg, NiSO 4 1mg, ZnSO 4 5mg, FeSO 4 5mg, MnSO 4 3 mg, CoSO 4 1 mg, Na 2 MoO 4 1 mg, H 3 BO 3 2mg, biotin 2 (VB 6 ) 10mg, thiamine (VB 1 ) 2mg, cyanocobalamin (VB 12 )0.1mg, pantothenic acid (VB 3) 5mg, folic acid 2mg, riboflavin 5mg, niacin 5mg, distilled water 1000mL, pH 6.4. Vitamins were added after filter sterilisation. ) was washed and centrifuged twice, the bacteria were suspended in SM culture medium, and inoculated in SMA culture medium according to the inoculum size of 5% (v / v) [Add filter-sterilized ACC (1-aminocyclopropane- 1-carboxylic acid deaminase) to a final concentration of 3 mM. ], cultur...

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Abstract

The present invention belongs to the technical fields of agriculture and environmental pollution treatment, and relates to endophytic bacteria capable of producing ACCD, its soil renovation bacterium agent and an application thereof. The endophytic bacteria Jp3-3 which is named as Pantoea agglomerans Jp3-3 and preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is 13th July, 2011, and the collection number of CCTCC is No: M 2011250. The ACCD enzymatic activity of the bacteria can reach as high as 402umM alpha-KB.h<-1>.mg<-1>; a liquid preparation contains effective live bacteria with amount of more than 1 billion / milliliter, a solid preparation contains live bacteria with amount of more than 0.2 billion / gram. The direct application of the soil renovation bacterium agent is capable of promoting the growth of the plants, absorbing the heavy metal and reinforcing the efficiency of plants for restoring the soil polluted by the heavy metal.

Description

technical field [0001] The invention belongs to the technical field of agriculture and environmental pollution control, and relates to an ACCD-producing plant endophytic bacterium, a soil remediation bacterium agent and an application thereof. Background technique [0002] The environmental problems caused by the mining of mineral resources and mine development are issues of global concern, and more and more people pay more and more attention to them, mainly because of the release of heavy metal toxic elements such as cadmium, lead, manganese, and zinc to the environment. The phytoremediation technology of heavy metal-contaminated soil has become a hot spot in the world because of its low cost and environmental friendliness, and it has become the best strategy for environmental restoration in mining areas. However, there are many problems in the implementation process, such as the low bioavailability of heavy metals in soil, which is not conducive to the absorption and remov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/01
Inventor 盛下放何琳燕黄智张艳峰张文辉
Owner NANJING AGRICULTURAL UNIVERSITY
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