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Pantoea agglomerans and application thereof

A technology of pantobacter agglomerates and bacteria, which is applied in the field of microorganisms to achieve the effects of being suitable for popularization, inhibiting growth and improving quality

Active Publication Date: 2016-01-06
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pantoea agglomerans (Pantoea agglomerans), as a biocontrol bacterium, can control the growth of pathogenic bacteria. At present, there are no related reports on the use of Pantoea agglomerans to control head blight and reduce the production of ZEN toxin and DON toxin.

Method used

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  • Pantoea agglomerans and application thereof
  • Pantoea agglomerans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 screening strains

[0027] Collect wheat ears in Liuhe area of ​​Jiangsu Province, after surface disinfection by conventional methods, grind wheat ears under aseptic conditions, add sterile water, mix well, perform serial dilutions, and apply different concentrations of dilutions on LB , PDA medium, cultured at 30°C for 5 days, pick different colonies on the plate, verify the antagonistic ability of different strains to Fusarium graminearum, pick the strains that inhibit the growth of Fusarium graminearum, in the early flowering stage of wheat Spray the antagonistic bacteria on the ears of wheat, harvest the wheat seeds after the wheat matures, detect DON toxin in wheat according to the method of national standard SN / 3136-2012, detect ZEN toxin in wheat according to the method of NY / T2070-2011, and finally To obtain a strain capable of simultaneously inhibiting the growth of Fusarium and reducing the production of gibberellin, the applicant named the obtained ...

Embodiment 2

[0031] Embodiment 2 prepares liquid bacterial agent

[0032] (a) Activate the Pantoea agglomerans strain AFXSJGH16 obtained in Example 1 in a petri dish, inoculate it in LB medium, and culture it on a test tube slope at 30°C for 48 hours to obtain a test tube strain;

[0033] (b) Pick the strains from the test tube and inoculate them into shake flasks containing LB medium, and cultivate them with shaking at 30°C and 150 rpm until the logarithmic growth phase to obtain the strains;

[0034] (c) Add 400L of LB medium to a 500L seed tank, then 121°C, 1.1kg / cm 2Autoclave for 20 minutes, cool to 30°C, add 10% of the volume of the LB medium into the strains obtained in step (b), and culture at a temperature of 28-33°C until the logarithmic growth phase, and the culture time is about For 48-60 hours, the stirring rate is 150-180rpm, during the cultivation period, sterile air is passed through, and the flow rate is 1:0.2-0.4 to obtain seed liquid;

[0035] (d) Add 4 tons of fermenta...

Embodiment 3

[0036] Embodiment 3 prepares wettable powder

[0037] Mix the Pantoea agglomerans liquid bacterial agent obtained in Example 2, diatomaceous earth, and attapulgite at a mass ratio of 1:1:1 to obtain a wettable powder of Pantoea agglomerans, and its bacterial content is not less than 10 10 cfu / g.

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Abstract

The invention provides pantoea agglomerans and application thereof. A preservation number of the pantoea agglomerans is CCTCC NO: M2015563. The thallus of the pantoea agglomerans is rod-shaped and is negative when being treated by Gram staining; the pantoea agglomerans can be prepared into a liquid bacterial agent or wettable powder and is used for preventing and treating wheat scab and reducing the content of wheat gibberellin; in a process of solving toxin pollution, no toxic byproducts are generated and secondary pollution is not caused, so that the quality of agricultural products is improved and the edible safety of people and livestock is ensured.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Pantoea agglomerans ( Pantoea agglomerans ) and its applications. Background technique [0002] Wheat head blight is a typical climate-type disease, which occurs in China, Japan, Southeast Asia, and the southern and northern parts of the United States, and the frequency of occurrence is gradually increasing. The region also has the characteristics of periodic epidemics, and is also called rotten wheat head, red wheat head, wheat ear dry etc. according to its pathogenic characteristics. With the promotion of no-tillage, straw returning planting techniques and crop rotation of wheat and corn in agricultural cultivation, the increase in the use of chemical fertilizers and the loss of resistance of wheat varieties, the incidence of the disease has increased year by year, and it has become a popular destructive disease in wheat fields. STDs. Since the 1990s, head blight ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 徐剑宏史建荣殷宪超徐圣佳周义东朱之薇
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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