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Complex microbial agent for low-temperature methane fermentation and preparation method thereof

A compound microbial agent and low-temperature fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of biogas production in biogas digesters in winter when decomposing straws, and no low-temperature fermentation of biogas has yet been seen. No need for a variety of products, troublesome use methods, etc., to achieve the effect of accelerated start-up, simple use methods, and strong digestion

Inactive Publication Date: 2012-05-02
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation of this method is that, firstly, focusing on decomposing the straw cannot solve the problem of biogas production in the user's biogas digester in winter; secondly, the compound bacterial agent can only be used in the pretreatment of the straw, and cannot be directly put into the biogas digester to promote biogas generation, and the method of use is cumbersome , limiting its promotion
[0010] According to the domestic patent search, there is no report on the microbial strains suitable for biogas low-temperature fermentation and related products for promotion and application.

Method used

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  • Complex microbial agent for low-temperature methane fermentation and preparation method thereof
  • Complex microbial agent for low-temperature methane fermentation and preparation method thereof
  • Complex microbial agent for low-temperature methane fermentation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Screening, domestication, breeding and classification of strains

[0039] Under the condition of 10℃, the dominant microorganisms in several differently treated sheep manure biogas reactors were separated, screened, cultured, identified and verified by back-connection experiments, and their effects were compared and analyzed to determine the types of fermentation bacteria. Simultaneously. Using molecular biology methods, the predominant flora of bacteria and fungi in the biogas fermentation tank was separated and identified, samples were collected and DNA extracted from the well-fermented sheep manure biogas reactor, and the 16S rDNA and 18S rDNA of the microbial population were analyzed. Identification and comparison and identification of microbial diversity to determine the type of flora.

[0040] Finally, two strains of R1 and R4 with obvious effects were determined.

[0041] Through the cultivation, separation and screening of microbial strains from the sheep ...

Embodiment 2

[0048] Example 2: Preparation of composite microbial agent for low-temperature fermentation of biogas

[0049] The present invention provides the fermentation broths of Acetobacter, Pseudomonas sp. CGMCC No. 4764 and Pantoea agglomerans CGMCC No. 4765 respectively according to slant culture and activation , Shake flask culture, seed tank liquid fermentation culture, fermentation tank liquid fermentation production, the production conditions are as follows:

[0050] Pseudomonas sp. CGMCC No. 4764 was cultured in LB liquid medium for 24 hours to prepare the strain, 200 ml of the strain was inoculated into 10 liters of PR medium; the fermentation temperature was 28 ℃, and NH 4 OH 28% solution adjusts the pH to 6-8 constantly, preferably 6.8; the stirring rate is 400rpm, the aeration rate is set to 0.4 (V / V); when the biomass concentration reaches 10g / L-40g / L, the fermentation is stopped .

[0051] Pantoea agglomerans (Pantoea agglomerans) CGMCC No. 4765 was cultured in R4F liquid mediu...

Embodiment 3

[0059] Example 3: Experimental study on biogas fermentation at 10°C, 15°C-18°C, and 30°C to produce gas

[0060] A 5L tank of sheep manure biogas fermentation bacteria inoculation test (adding 5-10 grams of bacteria) at 10℃, 15℃-18℃ (room temperature) and 30℃ were carried out, and the gas production conditions and gas production rate were tested. . The result is: as long as the temperature of the material liquid in the biogas fermentation device is maintained at more than 10°C, the biogas digester with cattle and sheep dung as the main raw material will increase by more than 30% compared with the non-infected biogas digester. figure 1 , 2 .

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Abstract

The invention discloses a complex microbial agent for low-temperature methane fermentation. According to the ccomplex microbial agent, fermentation broths obtained by fermenting pseudomonas sp. CGMCC (China General Microbiological Culture Collection) No.4764, pantoea agglomerans CGMCC No.4765 and pantoea agglomerans are uniformly mixed according to the weight ratio of 1:1:1, so that a liquid formis obtained. The complex microbial agent disclosed by the invention can be directly fed into a methane tank, so that the methane tank can be used for normally producing gas in winter; and in addition, the complex microbial agent has a wide application value.

Description

Technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a composite microbial agent used for producing biogas under low temperature conditions and capable of improving the use of cow dung and straw as raw materials and the technical field of preparation thereof. Background technique [0002] Under the concept of sustainable development, the Chinese government takes energy conservation and emission reduction as its main direction, and rural areas pay great attention to the construction of "agriculture, rural areas and farmers" and "ecological enrichment projects". Rural biogas has become an important link for the construction of rural ecological homes, the construction of a new socialist countryside, the realization of the recycling of agricultural resources, and energy conservation and emission reduction. It is closely related to the economic development of the entire rural society. [0003] Biogas is a ground-based bioma...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12P5/02C02F11/04C12R1/01C12R1/02C12R1/38
CPCY02E50/343Y02E50/30
Inventor 冯蕾杨新平陈竞马彩雯史慧锋秦新政唐娴
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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