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Pantoea for degrading organic phosphorus and inorganic phosphorus and application of pantoea

A technology of inorganic phosphorus and organic phosphorus, applied in the biological field, can solve the problems of soil phosphorus enrichment, and it is difficult for plants to directly absorb and utilize it.

Active Publication Date: 2015-04-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical phosphorus fertilizer applied to the soil has a utilization efficiency of 5%-25% this season, and most of the phosphorus is mixed with the Ca in the soil. 2+ , Fe 3+ 、Al 3+ Combined to form insoluble phosphate, it is difficult for plants to directly absorb and utilize, resulting in the phenomenon of phosphorus enrichment in the soil

Method used

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  • Pantoea for degrading organic phosphorus and inorganic phosphorus and application of pantoea
  • Pantoea for degrading organic phosphorus and inorganic phosphorus and application of pantoea
  • Pantoea for degrading organic phosphorus and inorganic phosphorus and application of pantoea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1. Isolation and identification of Pantoea sp. S-32CGMCC No.10017

[0072] 1. Isolation of Phosphorus Solubilizing Bacteria

[0073] 1. Soil sample collection

[0074] The reclaimed soil was collected from the Xiaoyi open-pit mining area in Shanxi Province (collection time: June 2012, collector: Li Jianhua, contact information: 0351-7639390).

[0075] 2. Primary screening

[0076] Add 10 g of the above-mentioned soil sample to a Erlenmeyer flask filled with 100 ml of sterile water, shake and incubate for 10 minutes to make a cloudy solution. Take 1ml of the above cloudy solution and add it to a test tube filled with 9ml sterile water and mix well (at this time, the dilution is recorded as 10 -1 ), then draw 1ml from this test tube and add it to another test tube filled with 9ml sterile water and mix evenly, and so on to make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Soil suspensions at different dilutions. will be 10 -4 、10 -5 、10 -6 1.0ml of each dilution o...

Embodiment 2

[0116] Example 2, Phosphatase activity secreted by Pantoea sp. S-32CGMCC No.10017

[0117]Get and measure the phosphatase activity of the supernatant after the pantoea (Pantoea sp.) S-32CGMCC No.100175d of embodiment 1 is cultivated with organophosphorus liquid medium, respectively adopt alkaline phosphatase kit (Nanjing Jiancheng Institute of Bioengineering , Cat. No. A059-1) and acid phosphatase kit (Nanjing Jiancheng Institute of Bioengineering, Cat. No. A060-1) were used to measure acid phosphatase activity and alkaline phosphatase activity according to the instructions. 100mL of fermentation broth was reacted with substrate (disodium phenyl phosphate) for 15min at 37°C to produce 1mg of phenol, which was defined as 1 Guinea unit (U). Bacillus megaterium As1.223 was used as the control, and the organophosphate liquid medium without adding any strain was used as the blank control.

[0118] The results showed that the acid phosphatase and alkaline phosphatase enzyme activit...

Embodiment 3

[0119] Example 3, Pantoea sp. (Pantoea sp.) S-32CGMCC No.10017 Environmental Adaptation and Determination of Phosphorus Solubilizing Ability

[0120] The phosphorus dissolving rate measuring method in the present embodiment is all the same as embodiment 1.

[0121] 1. Environmental adaptability of Pantoea sp. S-32CGMCC No.10017

[0122] The experiment was repeated three times, and the specific steps of each repeated experiment were as follows:

[0123] Cultivate, observe, and record the temperature adaptability of Pantoea sp. S-32CGMCC No.10017 at 4°C, 20°C, 28°C, 37°C, and 60°C respectively, using nutrient agar medium, and each treatment is performed 3 times Repeat; adjust the NaCl concentration of the nutrient agar medium to be 2%, 5%, 7%, 10%, 12%, 15% respectively, each treatment is repeated 3 times, cultivate, observe, and record the salt tolerance of the bacterial strain; adjust the nutrient agar The acidity of the culture medium was pH3, pH4, pH5, pH6, pH7, pH8, pH9, ...

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Abstract

The invention discloses pantoea for degrading organic phosphorus and inorganic phosphorus and application of the pantoea. The preservation number of the pantoea (Pantoea sp.)S-32 in a general microorganism center of the China Committee for Culture Collection of Microorganisms is CGMCC No.10017. According to the pantoea, the phosphorus solubility for degrading calcium phosphate reaches 24.19% and is equal to 3.64 times of that of bacillus megatherium As1,223; the phosphorus solubility for degrading lecithin reaches 2.47% and is equal to 1.46 times of that of bacillus megatherium As1,223; the pantoea has relatively strong environmental suitability and can adapt to relatively wide ranges of temperature, pH value and salinity. Experiments prove that the pantoea (Pantoea sp.)S-32 CGMCC No.10017 can serve as a strain resource of microbial fertilizers so as to increase the crop yield and accelerate the ecological restoration of soil of mining areas.

Description

technical field [0001] The invention relates to a pantoea that degrades organic phosphorus and inorganic phosphorus and its application in the field of biotechnology. Background technique [0002] Phosphorus is one of the essential nutrient elements for plant growth. Its importance is second only to nitrogen, accounting for about 0.2% of plant dry weight. It is the main component involved in energy metabolism, nucleic acid and cell synthesis, and some enzyme regulation, and it plays an important role in promoting plant growth. Development and metabolism play important roles and are irreplaceable components in the ecosystem. [0003] The average phosphorus content in the soil is about 0.05% (w / w). The phosphorus in the soil exists in two states of inorganic and organic compounds, but only 0.1% of the phosphorus can be used by plants, which becomes a limiting factor affecting plant growth. factor. Among them, insoluble organic phosphorus generally accounts for 20%-50% of the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 陈倩刘善江白杨李亚星郜春花李建华靳东升卢朝东
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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