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Using Pantoea agglomcrans KFS-9 fungus to produce 2, 3 butanediol, and application in cosmetic

A technology for butanediol and cosmetics, which is used in the production of 2,3-butanediol by Pantoea agglomerans KFS-9 bacteria and its application in cosmetics, and achieves the effects of high yield, strong anti-oxidation and length reduction.

Inactive Publication Date: 2007-05-09
OCEAN UNIV OF CHINA
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Problems solved by technology

There is no relevant report about its use in cosmetics

Method used

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  • Using Pantoea agglomcrans KFS-9 fungus to produce 2, 3 butanediol, and application in cosmetic
  • Using Pantoea agglomcrans KFS-9 fungus to produce 2, 3 butanediol, and application in cosmetic
  • Using Pantoea agglomcrans KFS-9 fungus to produce 2, 3 butanediol, and application in cosmetic

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Embodiment Construction

[0010] 1. Obtaining method of strain:

[0011] Collect samples from mangroves. After diluting the collected samples with sterile saline to a certain concentration, take 4ml of them and place them in a series of sterile small plates (6cm) to form a layer of bacterial suspension with a thickness of about 15mm on the bottom of the plates. Each series of small plates were irradiated with UV-B for a series of time (5-20min), and then coated on the modified peptone beef extract glucose medium, which will survive the maximum dose of radiation without spore structure The bacteria were separated and purified, and their activity was verified by passaging. Finally, a strain with anti-UV-B radiation activity was obtained, named KFS-9. The 16S rRNA gene sequencing technology combined with traditional physiological and biochemical characteristics was used to carry out the KFS-9 strain. After identification, it was finally determined to belong to Pantoea agglomerans (the composition of the impro...

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Abstract

This invention provides a strain for producing 2, 3-butanediol, and its application as anti-UV and anti-oxidation agent in cosmetics. The strain with strong UV tolerance is separated from mangrove, and can produce 2, 3-butanediol. The strain has such advantages as wide nutrition range, short culture period, low cost and high yield. 2, 3-butanediol has good anti-UV and anti-oxidation effects, and can eliminate active oxygen free radicals and block lipid peroxidation, thus can be used as anti-UV and anti-oxidation agent in cosmetics.

Description

Technical field [0001] The invention relates to a bacterium Pantoea agglomerans KFS-9 which produces 2,3-butanediol and the application of 2,3-butanediol in cosmetics. Background technique [0002] According to the inventors' knowledge by consulting data and literature search, the strains capable of producing 2,3-butanediol are: Klebsiella, Bacillus, Aeromonas, and Serratia. There is no report about the production of 2,3-butanediol by the strain Pantoea agglomerans involved in this patent. At present, only Klebsiella pneumoniae (Klebsiella oxytoca) and Bacillus polymyxa can be industrialized. The former has the highest final concentration of 2,3-butanediol produced by batch fermentation, which can reach 118g / L, and the yield is 24g / L·h. The amount of 2,3-butanediol produced is twice that of the latter, but The latter lacks the ability to saccharify starch. This patented strain adopts two-stage fermentation culture, the final concentration of 2,3-butanediol produced by fermentatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K8/34
Inventor 江晓路王洪媛管华诗
Owner OCEAN UNIV OF CHINA
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