Complex flora for preventing and treating peanut root rot and application thereof

A compound flora and peanut root technology, applied in the direction of application, microbial-based methods, chemicals for biological control, etc., can solve the problem of weak competitiveness of indigenous flora, unsatisfactory control effect, and the vulnerability of single strains to the outside world. Environmental interference and other problems, to achieve the effect of stable disease prevention, prevention and control of infested peanut roots, and optimization of the rhizosphere micro-ecological environment

Pending Publication Date: 2020-10-13
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, single strains are easily disturbed by the external environment and are weakly competitive with the indigenous flora, resulting in unsatisfactory control effects

Method used

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  • Complex flora for preventing and treating peanut root rot and application thereof
  • Complex flora for preventing and treating peanut root rot and application thereof
  • Complex flora for preventing and treating peanut root rot and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] The present invention has no special limitation on the preparation method of the complex flora. After culturing Pantoea, Pseudomonas, Enterobacter, Paenibacillus, Paenibacillus, Bacillus and Pseudomonas aeruginosa respectively, the cultured Mix the mixture evenly.

[0019] The present invention also provides the application of the composite flora described in the above scheme in the prevention and treatment of peanut root rot; It cannot effectively colonize in the rhizosphere of peanuts in continuous cropping, but exists in the rhizosphere of peanuts in rotation; the peanut root rot preferably includes peanut root rot caused by Fusarium oxysporum.

[0020] In the present invention, the application preferably includes the following steps: mixing and applying the complex flora and peanut seeds; and / or applying after mixing the complex flora and decomposed organic fertilizer.

[0021] In the present invention, the mass ratio of the complex flora and peanut seeds is prefer...

Embodiment 1

[0023] The acquisition of embodiment 1 peanut rhizosphere key flora

[0024] The peanut experimental field that has been continuously cropped for more than 5 years was selected as the test soil, and the experimental field where corn, soybean and peanut were rotated for the past five years was selected as the control experimental soil. The pathogenic bacteria tested was peanut root rot Fusarium (F.oxysporum) ACCC36194.

[0025] The rhizosphere soil was collected by root shaking method, and the rhizosphere bacteria of peanut were isolated by flat plate coating method.

[0026] First use a small steel shovel to remove the soil around the potted peanut plant, pull out the peanut carefully, shake the root system gently to remove large pieces of soil, and then use a sterile brush to collect the soil on the surface of the peanut rhizosphere; take 1g of the rhizosphere soil of the peanut in 50mL of sterile Add 9 mL of sterile water to the Erlenmeyer flask, shake at 25 °C, 180 r / min fo...

Embodiment 2

[0037] Example 2: Development of composite flora and its disease suppression experiment on pathogenic bacteria

[0038] Use NB medium to activate 7 continuous cropping peanut deletion strains, 3000g, centrifuge for 5min, remove the bacterial fermentation liquid), add sterile water to adjust the bacterial concentration to make the OD 600nm =1, spare. According to 7 kinds of strains, 2 kinds of strains were randomly combined and mixed, and there was 1 treatment for the 7-bacteria combination; 10 treatments for the 2-bacteria combination. Ensure that the concentration of each strain in each treatment is the same. Take 1mL of each strain into a 10mL sterile centrifuge tube, add sterile water to ensure the same concentration of each strain in each treatment, mix the bacterial suspension upside down several times, and set aside.

[0039] Further, the method for determining the combined pathogenicity of the 7 mixed strains is:

[0040] The direct confrontation method of the plate ...

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Abstract

The invention provides a complex flora for preventing and treating peanut root rot and an application thereof, and belongs to the technical field of soil-borne disease prevention and treatment. The complex flora comprises pantoea, pseudobacillus, enterobacter, paenibacillus, sporosarcina, bacillus and pseudomonas aeruginosa. The pantoea, the pseudobacillus, the enterobacter, the paenibacillus, thesporosarcina, the bacillus and the pseudomonas aeruginosa in the complex flora have a synergistic interaction effect on inhibition of pathogenic fungi; compared with a single strain, the complex flora has the advantages that the rhizosphere colonization capacity is obviously improved, and the rhizosphere micro-ecological environment can be effectively optimized. The complex flora is inoculated topeanut roots, so that the peanut roots can be effectively prevented from being infected by fusarium oxysporum, and the health of the peanut roots is protected.

Description

technical field [0001] The invention relates to a compound flora for preventing and treating peanut root rot and its application, and belongs to the technical field of soil-borne disease prevention and control. Background technique [0002] Peanut is an important oil crop in my country, and the annual sown area accounts for about 35% of the total sown area of ​​oil crops. Limited to the area of ​​arable land and the development of agricultural industrialization, the phenomenon of intensive peanut production is common, resulting in serious obstacles to continuous cropping. Continuous cropping of peanuts often leads to aggravation of soil-borne fungal diseases, among which root rot is the most serious disease in continuous peanut cropping in red soil areas, and is an important factor restricting local peanut production. [0003] At present, common measures to prevent and control soil-borne diseases include physical, chemical and biological methods. Among them, biological con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/25A01N63/27A01N63/20A01P3/00C12R1/01C12R1/07C12R1/385
CPCA01N63/20A01N63/22A01N63/25A01N63/27C12N1/20
Inventor 李孝刚王兴祥杨珍
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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