Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis

A technology for safety evaluation of Lygus spp., applied in animal husbandry and other fields

Active Publication Date: 2014-06-25
HUAZHONG AGRI UNIV
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Problems solved by technology

[0003] However, while the use of insect-resistant transgenic plants brings benefits to humans, there are also certain potential ecological risks.

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  • Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis
  • Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis
  • Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis

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Embodiment Construction

[0036] The Bt-gene insect-resistant rice of the present invention is selected from cry2Aa gene-transformed rice T2A-1, which has high resistance to lepidopteran pests. The non-transgenic parent is selected from Guangminghui 63 rice, and the rice seeds are all inherited from crops of Huazhong Agricultural University. Provided by the State Key Laboratory of Improvement. The rice was cultivated in a cultivation tray (length×width×height=25cm×20cm×3cm) with Yoshida nutrient solution to a height of about 15cm and used for the evaluation test. The conditions for cultivating rice seedlings are temperature (26±2°C) and relative humidity (80%).

[0037] The tested brown planthoppers of the present invention were collected from paddy fields in Wuhan, Hubei, and established populations on rice seedlings of T2A-1 and Minghui 63 respectively indoors, and reared for more than 10 generations. The green Lygus black-shouldered bug was collected from the paddy fields of Xiaogan, Hubei, and it ...

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Abstract

The invention relates to a safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis. The safety evaluation method includes the five parts including Tier-1 toxicity testing, three-level nutrition testing, protein transferring rules, predation function reaction and population density and population dynamic comparison. The potential toxicity of Bt protein expressed by Bt transgene insect-resistant rice on the cyrtorhinus lividipennis are respectively evaluated by the five parts; influences of the Bt transgene insect-resistant rice on the cyrtorhinus lividipennis on prey media are evaluated; paths and degrees for the cyrtorhinus lividipennis to be exposed in Bt protein are evaluated; influences of the Bt transgene insect-resistant rice on the cyrtorhinus lividipennis population are evaluated. Finally generated ecological effects of transgenic crop planting on non-target organisms are comprehensively evaluated according to data in the five parts. The safety evaluation method is systematic and scientific, and is of great significance on perfecting influence studying of transgenic crops on the non-target organisms and determining the ecological safety of transgenic crop planting.

Description

technical field [0001] The invention belongs to the technical field of ecological risk assessment of transgenic crops, and in particular relates to a method for evaluating the safety of transgenic insect-resistant rice against predatory natural enemy Lygus spp. Background technique [0002] Rice is one of the most important food crops in the world, and more than 3 billion people in the world depend on rice for food. At the same time, rice is one of the most pest-damaged food crops. Every year, due to the damage of rice leaf rollers, borer borers and other Lepidoptera insects, major yield losses are caused (Cheng, 1996; Nathan, 2006). In actual production, the control of lepidopteran pests has long relied on chemical pesticides. The abuse of chemical pesticides not only increases the production cost of rice, but also pollutes the environment. It is also easy to cause pests to develop resistance and reduce the efficacy of pesticides (Su et al. , 2003). Therefore, highly resi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 华红霞韩宇何月平赵景蔡万伦
Owner HUAZHONG AGRI UNIV
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