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Neoseiulus californicus low-temperature storage method

A low-temperature storage and mite-growing technology, which can be used in animal husbandry and other fields, and can solve the problem of time-consuming packaging work

Inactive Publication Date: 2015-03-11
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes a lot of time to subpackage Neoseius californica before refrigeration. The mite is active and small in size. When subpackaging it into each centrifuge tube, it cannot be directly picked into the centrifuge tube with a small brush, because the mite will Instantaneous climb out along the nozzle, unable to achieve the goal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] 1. Collection of female adults of Neoseius californica at the same time

[0012] Choose a disposable plastic lunch box that is clean and free of impurities, and lay a layer of saturated absorbent cotton inside. Put the blades of the sharp bean leaves that are severely infected with Tetranychus cinnabarinus on the absorbent cotton as a breeding container. The mature female adults of Neoseius californica live on the leaves of sword bean. After 12 hours, the eggs of the same period produced by Neoseius californica were collected. The collected eggs of the same period were placed in the above-mentioned new rearing container, and raised in an artificial climate box at 25° C. After 5 to 6 days, the first eclosion female adult mite of Neoseiusius californica could be obtained. When Neoseius californica develops to the second nymph, it is necessary to frequently observe whether any male mites have emerged, and the male mites should be picked out in time to avoid mating with fe...

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PUM

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Abstract

The invention discloses a neoseiulus californicus low-temperature storage method, and belongs to the field of agricultural insect biological control. According to the method, the collected neoseiulus californicus homochronous female adults are in split charge into 2ml plastic centrifugal tubes sterilized by two kinds of cold storage media, and the centrifugal tubes subjected to the split charging are respectively placed into cold storage rooms being 4 DEG C, 7 DEG C and 10 DEG C according to different cold storage substrate and cold storage periods, and are covered by black light-tight plastic films. The method provided by the invention has the advantages that the neoseiulus californicus can be stored for a long time at low temperature, under the condition of not influencing the survival rate, the breeding capability and the predation capability similar to those of the neoseiulus californicus which is not subjected to cold storage can be maintained, in addition, the predatory neoseiulus californicus is enabled to be protected from mechanical injury in the conveying process, and the guarantee is provided for the commercialized operation of the local neoseiulus californicus.

Description

technical field [0001] The invention relates to the field of biological control of agricultural insects, in particular to a low-temperature storage method for Neoseius californica. Background technique [0002] Acarids are the second largest group of arthropods after insects. Among them, spider mites are the ones that cause serious damage to agricultural and forestry production. Predatory mites have the ability to prey on harmful mites and pests. One of the most important natural enemies of commercial production. Neoseius californica is a predatory mite that is relatively obligate to spider mites. It can exert a strong control effect on a variety of spider mites, reproduce rapidly and have strong environmental adaptability. The population of Neoseius californica present in the Laboratory of Agricultural Insects and Pest Control of Sichuan Agricultural University is a local population in Sichuan, and was identified as a new domestic species by the Institute of Plant Protecti...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李庆税玲蒲颇马孝龙李颖蒋春先杨群芳王海建
Owner SICHUAN AGRI UNIV
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