Iterative gene circuit based on vibrio fischeri quorum sensing system and T7 expression system, and application of iterative gene circuit

A quorum sensing system and gene circuit technology, applied in the field of genetic engineering, can solve the problems of inability to achieve strict regulation of target genes, weak activation of quorum sensing system, and limited application of quorum sensing system, so as to save fermentation costs and reduce expression leakage. , the effect of enhancing vitality
CN110747217AActive Publication Date: 2020-02-04NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2020-02-04

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Abstract

The invention discloses an iterative gene circuit based on a vibrio fischeri quorum sensing system and a T7 expression system. The iterative gene circuit comprises a signal molecular protein gene luxIof vibrio fischeri, a receptor protein gene luxR, a promoter sequence PluxI(1) for sensing a signal molecular and receptor protein complex, a T7 RNA polymerase gene T7 RNApoly and a green fluorescentprotein egfp for representing the circuit intensity. The gene circuit does not rely on an inducer and can utilize the T7 expression system to spontaneously and forcefully initiate target gene expression in any host bacterium; and gene elements are constructed on different expression vectors, the iterative gene circuit is constructed in engineering bacteria, the T7 expression system is utilized toamplify signals of the vibrio fischeri quorum sensing system, and the iterative gene circuit has the function of utilizing the T7 expression system to spontaneously and forcefully initiate target gene expression, and has a lower leakage ratio and higher initiating strength.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to an iterative gene circuit based on a Vibrio fischeri quorum sensing system and a T7 expression system and an application thereof. Background technique

[0002] Fermentation engineering bacteria need to synthesize a large amount of recombinant protein and build a synthetic pathway. If a large amount of recombinant protein is expressed at the starting point of culture, it will compete with cell metabolism for intracellular resources, which will bring a huge burden to the bacteria. The imbalance of metabolic network will lead to some toxic intermediates. The accumulation of products will also cause toxicity to the growth of the bacteria, making the strain unable to maintain a good growth state and affecting the yield of the final product. Therefore, the predecessors have explored a variety of operating elements to achieve the induction and regulation of genes, includin...

Claims

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