16 results about "Gene Circuits" patented technology

Disclosed herein are nucleic acid constructs that can be used to build genetic circuits for producing antibodies comprising split toxins. Also disclosed herein are methods of producing platelets comprising the antibodies. The platelets produced by the methods disclosed herein can be used to target circulating tumor cells.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The invention discloses an application of a gene line DgCsp2-DrWhy in regulation and control of a rice tillering phenotype. The invention provides a method for preparing a transgenic plant with increased effective tillering number and / or plant height, which comprises the following step: introducing a DgCsp2 gene and a Drwhy gene into a receptor plant to obtain the transgenic plant with increased effective tillering number and / or plant height. The DgCsp2 gene is a gene for coding a protein as shown in SEQ ID NO: 1. And the Drwhy gene is a gene for coding a protein as shown in SEQ ID NO: 3. The method disclosed by the invention has application and popularization values for cultivating new plant germplasm, particularly new rice planting with increased tillering quantity and plant height.

Methods of detecting a target analyte in subsurface soil can providing a bacterial consortium embodying a genetic circuit. Such a consortium can include a sensor bacterium, a signal propagation bacterium, and a display bacterium, which are provided at a soil surface. The sensor bacterium and signal propagation can be allowed to migrate into subsurface soil below the soil surface. The sensor bacterium can be exposed to the target analyte in the subsurface soil. The sensor bacterium can produce a signal molecule in response to the presence of the target analyte. The signal propagation bacterium can amplify the signal molecule by producing additional signal molecules in response to the presence of the signal molecule. The display bacterium can produce an observable signal in response to presence of the signal molecule.

The application discloses a bacteria with limited reproduction capacity, a method for controlling bacterial reproduction and application, and belongs to the technical field of biotechnology and cell immunotherapy. The bacterial DNA comprises an unnatural amino acid orthogonal translation module, a suicide module and a rescue module. Based on the unnatural amino acid (ncAA) orthogonal translation system, the orthogonal genetic barrier theory is used, gene circuit design and element modularization are used, bacterial reproduction generations are accurately controlled, bacterial growth is effectively controlled, and the bacteria are prevented from escaping, and the bacteria have wide applicability. The bacteria and the method have wide application prospects in the development of attenuated live vaccines.

The invention relates to a T7 RNA polymerase mutant with K1F promoter recognition activity and application of the T7 RNA polymerase mutant in construction of gene circuit logic gate design. The T7 RNA polymerase mutant provided by the invention has RNA polymerase activity based on a K1F promoter, can recognize the corresponding K1F promoter, and keeps orthogonality with a T7 RNAP promoter on the full-length division polymerase variant and on the division polymerase variant with proximity dependency. Compared with the existing K1F RNA polymerase, the K1F RNA polymerase has the advantages of high specificity, enhanced RNA transcriptional activity and the like, the performance is obviously improved, the requirements of current gene circuit multi-path orthogonal regulation and control are met, and an effective candidate enzyme tool is provided for RNA research and application.

This invention relates to the field of gene editing technology, specifically to a gene circuit-based specific gene expression system and module, a pharmaceutical composition, and its applications. The system includes a first vector and a second vector. The first vector includes a first expression cassette containing a cell-specific promoter, a coding sequence encoding a transcriptionally activated fusion protein, and a regulatory region sequence downstream of the coding sequence. The regulatory region sequence is configured to form a response element in the 3' untranslated region of the fusion protein's mRNA after transcription. This response element binds to a specific long non-coding RNA within the silenced cell, leading to the degradation of the fusion protein's mRNA. The second vector includes a second expression cassette containing an associated promoter that can be activated by the transcriptionally activated fusion protein, and a target gene downstream of the associated promoter. Advantages: This ensures that the target protein is expressed only in target cells and not in cancer cells, avoiding adverse effects on non-target cells or tissues, and reducing treatment risks and side effects.

This invention discloses the construction and application of a highly robust quorum sensing gene circuit. The quorum sensing gene circuit exhibits more stable output performance under different fermentation pH conditions, demonstrating high robustness. The response promoters PHU3 and PEL of the quorum sensing gene circuit possess good quorum sensing performance unaffected by pH crosstalk and exhibit good specificity. Using this quorum sensing gene circuit to express an acid-resistant module can improve the acid tolerance and fermentation production capacity of the strain in acidic environments. This invention provides a quorum sensing gene circuit with greater industrial application value.

The application discloses a gene expression stabilization method and system based on sRNA feedback and non-periodic sampling regulation, relates to the technical field of gene expression optimization and regulation, and comprises the following steps: firstly, an sRNA-mediated feedback control gene circuit is designed and constructed; secondly, a state space model describing system dynamics is established, and minimization of the steady-state expression deviation of a target protein before and after ribosome disturbance is taken as an optimization target; through real-time calculation of a stability evaluation index, a non-periodic sampling strategy is used to dynamically judge a regulation triggering time; finally, when the triggering condition is met, the ribosome disturbance level is estimated in real time, and the optimal ribosome binding site strength adjustment value is calculated through solving an optimization problem and is implemented. The application can adaptively maintain the stability of target protein expression in a culture environment with dynamic competition of ribosome resources at a low intervention frequency, and significantly improves the robustness and output consistency of a synthetic biology system.

The invention provides a microbial production cell for the synthesis of a product, further comprising a charge-dependent genetic circuit whose expression confers a selective growth and / or survival advantage to those cells that synthesize the product, while limiting the proliferation of unproductive or non-productive escape cells.

The application discloses an exonuclease fusing an NTPase domain and application thereof, and belongs to the technical field of bioengineering. The exonuclease is a Ppl protein, which comprises a PHP domain at the N terminal and an NTPase domain at the C terminal, wherein the PHP domain has 3'-5' ssDNA exonuclease activity. The NTPase domain of the application regulates the exonuclease activity of the PHP domain through conformational change. Under the condition of high NTP concentration, the NTPase domain inhibits the activity of the PHP domain; when the NTP concentration decreases, the inhibition is released, and the exonuclease activity is significantly activated. The activated PHP domain shows specific cleavage activity to DNA substrates with 3'-hydroxyl overhang. The unique 'NTP concentration sensing and enzyme activity regulation' characteristics make it a core module for developing biological sensors, gene circuits and controllable nucleic acid tools.

UO2F2 biosensors, and related U-sensing and / or F-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and / or neutralize uranium and in particular bioavailable UO2F2.

The invention discloses an expression vector of an AND gate positive feedback gene loop as well as a construction method and synthetic biology application thereof. The expression vector comprises an ignition element P1, a first terminator Tnos and a second terminator Tnos, wherein the ignition element P1 comprises a heat shock promoter PGmhsp17.6L, a nucleotide sequence for coding a transcription factor XVE and a first terminator Tnos; and a power element P2 comprising a chemically induced promoter PLexA35S, a nucleic acid sequence encoding a transcription factor XVE II, and a second terminator T35S. Through cooperation of P1 and P2, a dual induction control positive feedback loop is constructed: when heat shock and a chemical inducer exist at the same time, the loop is started, and rapid accumulation of transcription factors is realized through self-activation of P2, so that downstream gene expression is driven at high intensity. The design effectively overcomes the problems of insufficient expression quantity of a single induction system and heavy burden of constitutive expression metabolism, avoids cytotoxicity, and can be universally applied to synthesis of various secondary metabolites.

This invention relates to the field of gene regulation technology, and discloses a method for constructing and applying a light-controlled CRISPRi gene regulation system. The light-controlled CRISPRi gene regulation system includes a biological circuit and a light source device; the biological circuit includes a light control module and a CRISPRi module; the light source device is an electronic device that emits red or green light. This invention introduces red and green light to precisely regulate CRISPRi, making its on / off state spatiotemporally specific. This invention helps to deepen the understanding of light-controlled gene circuits and has important guiding significance for constructing large-scale synthetic biology systems that can target multiple genes. In practical applications, the light-controlled CRISPRi gene regulation system can be integrated into cells, allowing it to turn the expression of specific genes on or off in real time as needed, regulating cellular behavior or metabolic pathways, saving production costs, and improving production efficiency.

The invention discloses a protein loop for targeted killing of CMS2 colon cancer cells as well as a related biological material and application thereof. The invention relates to the technical field of biology, and provides a protein complex for targeted killing of CMS2 type colon cancer cells, which comprises three chimeric proteins of an activation element, a killing element and a regulating element, and is characterized in that: (1) the activation element activates the killing element only in MYC and WNT signal pathway activated cells through a cutting effect; (2) while the regulating element and the activating element are mutually inhibited to form a positive feedback cycle, the cutting effect of the activating element on the killing element is feedforward inhibited, so that the selectivity of a gene loop is increased. The protein loop constructed by the invention can be introduced in a single transcript form, and has excellent integration. Compared with the prior art, the protein loop constructed by the invention can specifically kill CMS2 type colon cancer cells in a targeting manner compared with a gene loop depending on a riboswitch, and has important application value in treatment of CMS2 type colon cancer.

The application discloses a cell-free cloning reaction system, a kit and application thereof. The system comprises a cell lysate, an inserted DNA fragment and a linear carrier. The cell lysate is obtained from induced and lysed Escherichia coli, wherein a lambda Red system and a ligase system are expressed. The lambda Red system comprises Exo protein, Beta protein and Gam protein. The ligase system comprises DNA ligase. The optimized lambda Red system and the high-efficiency DNA ligase system can complete high-efficiency splicing of multi-fragment DNA within 15 minutes at 37 DEG C, and still maintain a high positive rate in a multi-fragment assembly system, which is superior to Gibson cloning and expands the applicability in complex pathway synthesis and gene circuit reconstruction.