Single-subunit RNA polymerase and its purification method and application in RNA synthesis

A technology of RNA polymerase and purification method, applied to single-subunit RNA polymerase, its purification method and application field in RNA synthesis, can solve problems such as uneven transcription products
CN108018271AActive Publication Date: 2018-05-11RNASYN BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
RNASYN BIOTECH CO LTD
Publication Date
2018-05-11

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Abstract

The invention discloses a single-subunit RNA polymerase and its purification method and application in RNA synthesis. The single-subunit RNA polymerase is a single-subunit RNA polymerase derived froma phi KMV phage or an other phage single-subunit RNA polymerase with a protein sequence which is 25% or more homologous to the single-subunit RNA polymerase derived from the phi KMV phage, contains acharacteristic amino acid sequence shown in the formula of SEQ ID NO. 1 in the sequence table and has the total amino acid sequence number of 800 and 830. The research result shows that the RNA polymerase has a long-distance relationship with the existing RNA tool enzyme and high transcription efficiency. Compared with the existing T7 RNA polymerase and Syn5 RNA polymerase, the single-subunit RNApolymerase solves the problem that the synthesis of RNA rich in a stable and high-level structure from the T7 RNA polymerase and Syn5 RNA polymerase produces complex products. The single-subunit RNA polymerase produces same transcription products.
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Description

technical field

[0001] The invention belongs to the research field of microbial nucleic acid metabolizing enzymes, in particular to single-subunit RNA polymerase, its purification method and its application in RNA synthesis. Background technique

[0002] RNA (ribonucleic acid) is the most important class of macromolecules for the transmission of genetic information in all organisms, and it also plays a variety of regulatory roles in organisms. RNA research has become one of the hottest fields in biological research in recent years. RNA mainly includes mRNA, tRNA, rRNA and other categories. With the rapid development of biological technology in recent years, scientists have discovered some novel RNAs such as microRNA (miRNA) (Cheng et al., 2005), long non-coding RNA (lncRNA) (Shi et al., 2016), nanostructure RNA (Geary et al., 2014), etc. also play a vital role in organisms. The designed RNA sequence can encode and be translated into any protein, so it can theoretically rep...

Claims

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