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Casein-derived antioxidant peptide and preparation method thereof

A technology of antioxidant peptides and casein, applied in the field of bioactive peptides, can solve problems affecting physiological activity and antioxidant peptides

Active Publication Date: 2016-01-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, before these bioactive peptides reach the blood circulation through oral administration, they will be hindered by a series of obstacles, such as the low-acid environment in the stomach and pepsin, and the alkaline environment in the intestine and trypsin, peptidase, etc. These factors will affect the antioxidant capacity. Peptide absorption thus affects its physiological activity in vivo

Method used

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  • Casein-derived antioxidant peptide and preparation method thereof
  • Casein-derived antioxidant peptide and preparation method thereof
  • Casein-derived antioxidant peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Casein antioxidant peptides were prepared by hydrolyzing casein with alkaline protease.

[0067] (1) Weigh 30 g of casein, immerse in 600 mL of deionized water with a pH of 8.0, preheat in a 60°C water bath for 10 min, and prepare a casein sample solution;

[0068] (2) Then add 0.6g of alkaline protease with an enzyme activity of 2.4U / g, and put it in a water bath at 60°C for 4 hours; h. At 4.0 h, the pH of the hydrolyzate was adjusted to 8.0 with 1M NaOH aqueous solution; a hydrolyzate rich in antioxidant peptides was obtained.

[0069] (3) After the hydrolysis ends, the hydrolyzed solution prepared in step (2) is heated in a boiling water bath at 100° C. for 15 minutes to passivate the enzyme, and after being cooled to room temperature, its pH is adjusted to 7.0 by using 1M hydrochloric acid solution; The final casein hydrolyzate was vacuum concentrated to a dry matter content of 40%.

[0070] (4) Dissolve the concentrated solution in step (3) in 1 mL of acetic acid...

Embodiment 2

[0072] Casein antioxidant peptides were prepared by hydrolyzing casein with papain.

[0073] (1) Weigh 30 g of casein, immerse in 450 mL of distilled water with a pH of 7.0, preheat in a water bath at 50°C for 10 min, and prepare a casein sample solution;

[0074] (2) Next, 0.6 g of papain was added, and put into a water bath at 50° C. for 3 hours for hydrolysis; a hydrolyzate rich in antioxidant peptides was obtained.

[0075] (3) After the hydrolysis ends, the hydrolyzed solution prepared in step (2) is heated in a boiling water bath at 100° C. for 15 minutes to passivate the enzyme, and after being cooled to room temperature, its pH is adjusted to 7.0 by using 1M hydrochloric acid solution; The final casein hydrolyzate was vacuum concentrated to a dry matter content of 30%.

[0076] (4) Dissolve the concentrated solution in step (3) in a Tris-HCl buffer solution with a pH of 8.3, filter it with a 0.45 μm water filter, and take 2 mL of the filtrate to separate on a DEAE-Sep...

Embodiment 3

[0078] Casein antioxidant peptides were prepared by hydrolyzing casein with trypsin.

[0079] (1) Weigh 60 g of casein, immerse in 600 mL of pure water with a pH of 7.5, preheat in a 37°C water bath for 10 min, and prepare a casein sample solution;

[0080] (2) Next, 1.2 g of trypsin was added, and hydrolyzed in a water bath at 37°C for 3 hours; a hydrolyzate rich in antioxidant peptides was obtained.

[0081] (3) After the hydrolysis ends, the hydrolyzed solution prepared in step (2) is heated in a boiling water bath at 85°C for 25min, and after being cooled to the passivation room temperature of the enzyme, its pH is adjusted to 7.0 with 1M hydrochloric acid solution; The final casein hydrolyzate was vacuum concentrated to a dry matter content of 30%.

[0082] (4) Dissolve the concentrated solution in step (3) in 2 mL of acetic acid buffer solution with a concentration of 20 mM and a pH of 4.0. After fully dissolving, filter it with a 0.45 μm water filter, and take 1 mL of ...

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Abstract

The invention relates to the technical field of bioactive peptides, in particular to a casein-derived antioxidant peptide mixture. Casein is hydrolyzed with protease, various pre-products of the casein antioxidant peptide are obtained, then separation, desalination and concentration are performed through ion exchange column chromatography, and the casein antioxidant peptide rich in basic amino acid is obtained. The casein antioxidant peptide has higher cell antioxidant capacity and low-density lipoprotein oxidation inhibition capacity and can still keep good antioxidant capacity after being digested and absorbed by gastrointestinal tracts, so that the casein antioxidant peptide rich in basic amino acid can be applied to fields of food and healthcare products and has very high social and economic benefit.

Description

technical field [0001] The invention relates to the technical field of bioactive peptides, in particular to a casein-derived antioxidant peptide mixture rich in basic amino acids and a preparation method thereof. Background technique [0002] Low-density lipoprotein (LDL) is a lipoprotein particle that carries cholesterol into peripheral tissue cells, and can be oxidized into oxidized low-density lipoprotein (OX-LDL). When low-density lipoprotein, especially oxidized When the modified low-density lipoprotein is excessive, the cholesterol it carries will accumulate on the artery wall, causing atherosclerosis. Therefore, inhibiting the oxidation of LDL by supplementing with additional antioxidants is a very feasible way to prevent atherosclerosis. Although synthetic antioxidants are efficient and cheap, people prefer to choose natural antioxidants because of their side effects. For example, antioxidant peptides have attracted much attention due to their good effects in preve...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C12P21/02C07K1/18A23L33/18A61K38/10A61K38/08A61P9/10
Inventor 李博王婵王波霍艳姣
Owner CHINA AGRI UNIV
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