Functional peptide fiber, production method thereof and method for recovering peptide chains

a technology peptide chains, which is applied in the field of functional peptide fibers, production methods thereof, and methods for recovering peptide chains, can solve the problems of not knowing a method effective in incorporating functionality into fibers, and it becomes difficult to constitute peptide fibers stably

Inactive Publication Date: 2003-08-28
FUJIFILM BUSINESS INNOVATION CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0064] It is particularly desirable to connect the functional material to a connecting substance which connects the functional material to the peptide chain terminal biochemically specifically. This is because the peptide chain terminal can be chemically modified easily in comparison with other parts of the peptide chain, and the connecting position of the functional material in the functional peptide fiber can be easily controlled by connecting a biochemically specific connecting substance to this terminal.

Problems solved by technology

However, each of the peptide fibers existing in the natural world is generally constituted by folding of one peptide chain at every length of .beta.-strand, and nothing is known about a method effective in incorporating functionality in the fibers.
In case that the number of residues exceeds 200, folding of the peptide chain cannot be effected suitably so that it sometimes becomes difficult to constitute a peptide fiber stably.

Method used

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  • Functional peptide fiber, production method thereof and method for recovering peptide chains
  • Functional peptide fiber, production method thereof and method for recovering peptide chains
  • Functional peptide fiber, production method thereof and method for recovering peptide chains

Examples

Experimental program
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Effect test

example 1

[0111] (1) Synthesis of Peptides Having .alpha.-Helix Structure

[0112] Peptides shown in the following were designed and synthesized into amphipathic 2.alpha.-helix structure. 1

[0113] Adamantane carbonyl group was introduced into the amino terminal as a hydrophobic group to obtain a peptide (Ad-2.alpha.). In the same manner, a peptide (Bx-2.alpha.) whose amino terminal was biotinylated were designed and synthesized. They were formed into a parallel double chain peptide by intermolecular disulfide bond between cysteine residues of their carboxyl terminals. Single chain Ad-1.alpha. and Bx-1.alpha. were also synthesized. Their synthesis was carried out by the general 9-fluorenylmethoxycarbonyl (Fmoc) solid phase method described, e.g., by Takahashi, Y., Ueno, A. and Mihara, H., in Chem., Eur. J., 4, pp. 2475-2484. That is, Ad-1.alpha. and Bx-1.alpha. were synthesized by elongating Fmoc amino acid derivatives (each in 3 to 6 equivalent) corresponding to their amino acid sequences on Rink...

example 2

[0120] A functional fiber in which intervals between functional materials were controlled was obtained by carrying out the same procedure of Example 1, except that both of the peptide (Ad-2.alpha.) in which adamantane carbonyl group was introduced into the amino terminal as a hydrophobic group and the peptide (Bx-2.alpha.) whose amino terminal was biotinylated, obtained in Example 1(1), were used in 1(2) (from 1 to 1,000 .mu.M in concentration, from 1:1 to 100:1 in molar ratio).

example 3

[0121] When a peptide in which its amino terminal was biotinylated and further connected with avidin protein was produced instead of the peptide (Bx-2.alpha.) whose amino terminal was biotinylated in Example 1(1), and it was used in 1(2), it was able to form it into a .beta.-peptide fiber.

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Abstract

This invention provides a functional peptide fiber which comprises a plurality of peptide structure units each containing at least one peptide chain, wherein peptide chains contained in each adjacent peptide structure units do not form peptide bond but are structured into a fibrous form by taking a beta-sheet structure, and wherein at least one of the plurality of peptide structure units contains a peptide chain having a functional material connected thereto. Also disclosed are a method for producing the functional peptide fiber and a method for recovering peptide chains from the functional peptide fiber.

Description

[0001] This invention relates to a method for producing a functional peptide fiber having a desired function and to a method for recovering peptide chains.DESCRIPTION OF THE RELATED ART[0002] .alpha.-Helix structure and .beta.-sheet structure are known as possible secondary structures of peptide chain in which a large number of amino acid residues are peptide-bonded. The three-dimensional structure of various proteins is composed mainly of these .alpha.-helix structure and .beta.-sheet structure. One strand of amino acid sequence constituting a protein has a structure in which .alpha.-helix and .beta.-sheet are intricately connected, and a large number of studies have been reported on the relationship between these amino acid sequences and tertiary structures.[0003] Three-dimensional structure of protein is roughly classified into (1) spherical and (2) fibrous. Proteins such as enzymes and antibodies take a spherical structure, and the fibrous structure can be found in muscle fiber ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K1/02C07K1/04C07K7/08C07K14/00C07K19/00
CPCC07K14/001Y10T428/249948
Inventor MIHARA, HISAKAZU
Owner FUJIFILM BUSINESS INNOVATION CORP
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