Beta-glucan peptide and its preparing process and application

A technology of glucan peptide and dextran, which is applied in the field of β-glucan peptide and its preparation and application, can solve the problem of ineffective use as immunomodulators and anticancer agents, lack of effective methods, and product purity Not advanced question

Inactive Publication Date: 2005-09-07
OCEAN UNIV OF CHINA +1
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  • Abstract
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Problems solved by technology

At present, there is no effective method for preparing β-glucan peptide from Grifola frondosa, and the re...

Method used

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  • Beta-glucan peptide and its preparing process and application
  • Beta-glucan peptide and its preparing process and application

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Embodiment Construction

[0014] Add 1000 milliliters of 95% ethanol aqueous solution to 100 grams of Grifola frondosa mycelia, extract at 80°C for 2 hours, collect the precipitate by centrifugation, then add 1500 milliliters of distilled water to the precipitate, and extract at 100°C for 2 hours under reflux, centrifuge Collect the precipitate, then add 1000 milliliters of 5% (percentage by weight, the same below) NaOH aqueous solution to the precipitate, stir and extract at 4° C. for 6 hours, centrifuge to collect the clear liquid, and the clear liquid is neutralized with acetic acid and centrifuged to collect the supernatant, Concentrate and desalt the latter by ultrafiltration, add absolute ethanol to make the ethanol concentration 85%, collect the precipitate by centrifugation, and further separate the precipitate with Q-Sepharose FF column chromatography, and carry out gradient elution with an aqueous solution containing sodium chloride (pH7) , to obtain β-glucan peptide, its molecular weight is 1...

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Abstract

A kind of beta-dextrantide, which is characterized in that the main chain is beta-1, 6-dextran and the side chain is beta-1, 3-dextran in dextrose chain. Defatting the gray-ramali cmycelium with ethanol, extracting with hot water and alkaline, neutralizing with acid, ultrafiltering, condensing and desalinizating, adding ethanol for deposition, separating by column chromatography, eluting using salinity gradient solvent, gathering and merging according to elution peak, ultrafiltering, condensing, desalinizating and drying. The invention can remove the mixed protein, heterosaccharide, and other impurity substance by separating for only one time and can get beta-dextrantide with high purity, simplifying the process and improving the preparing velocity and yield; and at the same time avoids the shortage of destructing the peptide structure when employing enzymatical method. The product can also be used for preparing immuno-modulatory agents and anti-cancer drugs.

Description

technical field [0001] The invention relates to a method for preparing β-glucan peptide from the mycelia of Grifola frondosa and its application in immune regulation and anticancer. Background technique [0002] Grifola frondosa (Grifola. frondosa) belongs to Basidiomycetes, Polyporus, Basidiomycetes, and is a rare edible and medicinal fungus. A large amount of Grifola frondosa mycelium can be obtained by liquid fermentation process. Studies have shown that Grifola frondosa polysaccharides have a wide range of pharmacological activities, but the polysaccharides prepared from Grifola frondosa fruit bodies and fermented mycelia have different structures, and the structures of Grifola frondosa polysaccharides obtained under different culture conditions are also different; Different structures have different activities. In a Chinese patent (CN1398898A), a method for preparing polysaccharide peptides from fruiting bodies of Grifola frondosa is disclosed. The method adopts water ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P35/00A61P37/02C07K1/16C07K14/37
Inventor 于广利徐泽平赵峡李春林
Owner OCEAN UNIV OF CHINA
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