5results about How to "Metabolic stability" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to a rapid tissue propagation method for radix ranunculi ternati. The rapid propagation method for the radix ranunculi ternati tissue comprises the following steps: (1) seed selection and disinfection: selecting vigorous seeds, cleaning the seeds, soaking the seeds with mercury bichloride, disinfecting the seeds with alcohol, and finally washing the seeds with sterile water; (2) preparing a primary induction culture medium: preparing the primary induction culture medium; (3) seed induction and proliferation: inoculating the disinfected seeds on a primary induction culture medium, and culturing; after 4-6 weeks of culture, the seeds germinate into plantlets; transferring the plantlets to a subculture induction medium for multiplication culture, and subculturing once every 3-4 weeks; (4) rooting culture: transferring the proliferation seedlings to a rooting culture medium, and culturing for 2-3 weeks until the adventitious buds can root to form complete plants; and (5) seedling hardening and transplanting. The method can improve the rooting rate and shorten the propagation time at the same time.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The application discloses a method for treating harmful substances in breeding waste. The method comprises the following steps: degrading vitamins by using a compost anaerobic fermentation method, separating heavy metals by using a composite chelating agent and an EKR-PRB device, killing pests in the manure pile by using a composite microbial agent, and finally adjusting the system environment by using a stabilizer, so that the treated manure pile is easy to be recycled.

The present application relates to the field of microbial technology, in particular to Citrobacter werkmanii and its application. The present application provides Citrobacter werkmanii SDT3 with dual functions of denitrification and cobalt (III) reduction under aerobic conditions, and the preservation number is CCTCC NO: M 2025956. The strain can efficiently realize aerobic denitrification reduction of nitrate and nitrite under aerobic conditions without additional ammonia nitrogen, and simultaneously complete the synchronous reduction of cobalt (III) -histidine complex (Co(III)His). The strain is suitable for treating flue gas denitration failure absorption liquid containing Co(III)His, nitrate and nitrite, has the advantages of high absorption liquid regeneration efficiency, mild process conditions, wide application range of organic carbon source, no secondary pollution, easy engineering application, etc.; can provide an efficient and stable microbial technology solution for efficient regeneration of flue gas denitration absorption liquid, long-term continuous and stable operation of the denitration system.