Tobacco 7-dehydrocholesterol reductase gene and application thereof

A technology of dehydrocholesterol and reductase, applied in the field of tobacco genetic engineering, can solve problems such as few reports
CN113278637APending Publication Date: 2021-08-20CHINA TOBACCO YUNNAN IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA TOBACCO YUNNAN IND
Publication Date
2021-08-20

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Abstract

The invention relates to a tobacco 7-dehydrocholesterol reductase gene and application thereof. The nucleotide sequence of the 7-dehydrocholesterol reductase gene is as shown in SEQ ID NO. 1. Based on the important influence of sterol on the safety of tobacco leaves, the tobacco sterol metabolism regulation gene is deeply studied, so that a new tobacco variety is constructed by utilizing genetic engineering, and a good application foundation is laid for improving the tobacco variety. According to the application, preliminary research on specific tobacco sterol 7-dehydrocholesterol reductase shows that the specific tobacco sterol 7-dehydrocholesterol reductase is highly related to the sterol content in tobacco leaves, when the gene is silenced in Bensi tobacco, the stigmasterol content and the campesterol content in the tobacco leaves are reduced, and the total sterol content is reduced by 24.8%. Based on the characteristic, a certain foundation and reference can be provided for tobacco quality regulation and control and new tobacco variety cultivation.
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Description

technical field

[0001] The invention belongs to the field of tobacco genetic engineering, and in particular relates to a 7-dehydrocholesterol reductase gene NtDHCR7 of tobacco sterol synthesis and metabolism and application thereof. Background technique

[0002] Sterols belong to macromolecular alcohols and are a class of steroidal compounds that widely exist in nature. Sterols in nature are divided into animal sterols, mycosterols and phytosterols according to their sources. Phytosterols are important components of biofilm systems, which can regulate plant growth and development and respond to various biotic and abiotic stresses. Sterols account for about 0.1% to 0.3% of the mass of tobacco leaves. It has been confirmed by literature that sterols in tobacco are important precursor compounds of benzopyrene in cigarette smoke. Sterols in tobacco mainly include cholesterol, stigmasterol, campasterol, and β-sitosterol. Therefore, reducing the content of sterols in tobacco le...

Claims

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