A strain of methylobacter, method for separating and culturing and its application

A Methylobacillus and culture technology, applied in the field of new microbial strains, can solve problems such as carcinogens, and achieve the effect of reducing the content of sterols and the content of harmful substances

Active Publication Date: 2022-07-26
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a strain of Methylobacterium, method of isolation and cultivation and application thereof, to solve the problem that sterols in tobacco produce carcinogens after pyrolysis at high temperature

Method used

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  • A strain of methylobacter, method for separating and culturing and its application
  • A strain of methylobacter, method for separating and culturing and its application
  • A strain of methylobacter, method for separating and culturing and its application

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Experimental program
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Embodiment 1

[0038] 1. Preparation of culture medium:

[0039] Stigmasterol mother liquor: 25g of stigmasterol was added to 1L of water, 2ml of DMSO (dimethyl sulfoxide) was added, sterilized after ultrasonic solubilization, and stored in a refrigerator at 4°C.

[0040] Tobacco culture medium solid plate: take 20g of tobacco stems or tobacco leaves, add 400ml of water to extract at 65°C for 1 hour, filter to remove solids to obtain a liquid culture medium, add 4g of agar, sterilize with high pressure steam at 121°C for 20min, and prepare by cooling Obtain solid medium plates.

[0041] Seed medium solid plate with sterol as the only carbon source: 25g / L stigmasterol mother liquor: 160ml, citric acid: 2g, ferric ammonium citrate: 0.05g, dipotassium hydrogen phosphate: 0.5g, anhydrous magnesium sulfate: 0.5g , Ammonium nitrate: 32g, deionized water was added to 1L, agar 30g. pH 7.5, sterilized by high pressure steam at 121 °C for 20 min, and cooled to prepare seed medium.

[0042] Seed med...

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Abstract

The invention relates to a Methylobacterium sp., a method for separating and culturing the same, and an application thereof, Methylobacterium sp. WGM 16, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2019336. The bacterial strain of this technical solution is enriched by separating tobacco sewage, using sterol mother liquor, and culturing by using tobacco medium containing sterol as carbon source and seed medium with sterol as sole carbon source, respectively. obtained from the separation. The use of the strain can effectively reduce the sterol content in tobacco products, thereby reducing the content of harmful substances formed by sterol substances in the combustion process, and achieving the purpose of reducing coke and harm in tobacco products.

Description

technical field [0001] The invention belongs to the technical field of microbial strains, and in particular relates to a new microbial strain which is cultured and separated from tobacco sewage and its application. Background technique [0002] Tobacco contains about 0.3% phytosterols. Studies have shown that about 20 to 25% of tobacco phytosterols are completely transferred into the smoke and cause harm through thermal cracking. The sterols in tobacco can produce carcinogenic condensed aromatic hydrocarbons after high temperature cracking, and benzopyrene is the most typical. These harmful substances can enter the mainstream smoke. It is then absorbed by the human body, thereby causing harm to human health. Therefore, suitable biological methods are used to degrade sterols in tobacco into small molecular organic acids or finally into CO. 2 It is expected to improve the sensory quality of tobacco and reduce the harm of tobacco, which has important application value and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02C12R2001/01C12N1/205
Inventor 张展叶建斌王璐陈小龙郭霖轩王金棒杨宗灿冯颖杰张婷婷杨晨李倩
Owner CHINA TOBACCO HENAN IND
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