Tobacco squalene synthase protein, tobacco squalene synthase gene and application thereof

Abstract

The invention discloses a tobacco squalene synthase?protein, the?amino acid sequence?of which is as shown in SEQIDNO: 1. Tobacco squalene synthase?gene (i) NtSQS ( / i) is a?key regulatory gene?in tobacco sterol synthesis pathway?and has a specific high expression level in?tender?tissue.?Virus mediated?gene silencing?vector TRV-NtSQS which is constructed is?inoculated to?Ben's tobacco?so as to obtain a plant with high efficiency of silence. By detection of?phytosterol?content in?Ben's tobacco, the phytosterol?content in the?gene silencing?plant is found to be?observably?decreased?by 41.26%. The above?phenomenon?indicates that?the sterol content?in a plant can be significantly reduced by silencing?the gene. Tobacco squalene synthase?gene (i) NtSQS ( / i) is the?key regulatory genein tobacco sterol synthesis pathway, can be used to adjust the sterol content?in tobacco and contributes to?genetic engineering breeding?of low sterol?high quality tobacco.

Description

technical field [0001] The invention relates to tobacco sterol synthesis and regulation key genes, in particular to the application of tobacco squalene synthase gene NtSQS in reducing tobacco sterol content. Background technique [0002] Tobacco is a plant of the genus Nicotiana in the family Solanaceae of the dicotyledonous plant class Picoflowers. There are more than 60 species of Nicotiana. Among the two cultivars, common tobacco (also known as Nicotiana tabacum) occupies the main area, and Nicotiana rustica occupies a small area. Cultivated tobacco can be divided into six types: flue-cured tobacco, sun-cured tobacco, air-dried tobacco, burley tobacco, oriental tobacco and yellow flower tobacco according to the quality characteristics of tobacco leaves, biological characteristics and cultivation methods, among which flue-cured tobacco is the most widely cultivated common tobacco. my country's flue-cured tobacco planting area and total output both rank first in the world....

AI Technical Summary

Problems solved by technology

Since the structure of sterols all contain hydroxyl groups, the tetracyclopentene[α]phenanthrene ring structure of its parent can form fused-ring aromatic hydrocarbons during pyrolysis. Therefore, sterols in tobacco are potential substances that affect human health, so reduce The sterol content in mature tobacco leaves is also one of the effective ways to reduce the tar and harm of cigarettes. At present, there is no effective method to effectively reduce the sterol content in tobacco.

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