Method for degrading sterol compounds in tobacco through microorganism combination method

A technology of microbial degradation and sterols, which is applied in the fields of tobacco, tobacco processing, food science, etc., can solve the problems of slow growth rate and low efficiency of sterol degradation, and achieve the effect of improving tolerance

Active Publication Date: 2018-05-22
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many studies have found that although mycobacteria can reproduce in tobacco extracts, their growth rate is slow and their sterol degradation efficiency is low.

Method used

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  • Method for degrading sterol compounds in tobacco through microorganism combination method
  • Method for degrading sterol compounds in tobacco through microorganism combination method
  • Method for degrading sterol compounds in tobacco through microorganism combination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Cultivation of Arthrobacter and Mycobacterium

[0025] 1.1 Arthrobacter

[0026] (1) Arthrobacter growth medium: beef extract 5g, egg white 10g, NaCl 5g, nicotine 2g, agar 15g, add water to make the volume to 1L, adjust the pH to 7.0, and sterilize at 121°C for 20min.

[0027] (2) Arthrobacter strain: Inoculate the seed solution of Arthrobacter into the growth medium with 10% inoculum, and cultivate at 30°C and 200rpm for 24 hours to obtain the Arthrobacter strain. The concentration of Arthrobacter in the strain is 10 9 Pieces / mL.

[0028] 1.2 Mycobacterium

[0029] (1) Mycobacterium liquid growth medium: glucose 10.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NH 4 NO 3 2g, 0.05g ferric amine citrate, 2.0g citric acid, 5.0g glycerol, add water to make the volume to 1L, adjust the pH to 7.0-7.2, and sterilize at 121℃ for 20min (prepare solid plate medium and add liquid growth medium mass fraction 2% agar).

[0030] (2) Mycobacterium resting cell liquid: inoculate cryoprese...

Embodiment 2

[0032] The method for degrading sterols in tobacco by the microbial combination method of the present invention includes the following steps:

[0033] (1) Take 30g each of the tobacco stems and dust, extract 20 times the weight of water at 55°C for 1 hour, and then pass through a 200-mesh screen for solid-liquid separation, and the resulting liquid is the tobacco extract;

[0034] (2) According to 10% of the inoculation amount, add Arthrobacter bacterium liquid to the tobacco extract and ferment at 30°C and 200 rpm to reduce the nicotine content in the tobacco extract so that the nicotine content is less than 0.5g / L;

[0035] (3) Add the resting cell liquid of mycobacteria to the tobacco extract of step (2) according to the 10% inoculum, and ferment at 37°C and 200 rpm for 48 hours to reduce the sterols in the tobacco extract content.

Embodiment 3

[0037] The method for degrading sterols in tobacco by the microbial combination method of the present invention includes the following steps:

[0038] (1) Take 30 g of tobacco leaves, use 30 times the weight of water, extract for 70 minutes at 50°C, and then pass through a 200 mesh screen for solid-liquid separation, and the obtained liquid is the tobacco extract;

[0039] (2) According to the inoculation amount of 12%, add Arthrobacter bacteria liquid to the tobacco extract, and ferment at 25°C and 250rpm to reduce the nicotine content in the tobacco extract so that the nicotine content is less than 0.5g / L;

[0040] (3) According to the inoculation amount of 12%, add the resting cell liquid of mycobacteria to the tobacco extract of step (2), and ferment at 35°C and 250 rpm for 50 hours to reduce the sterols in the tobacco extract content.

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Abstract

The invention discloses a method for degrading sterol compounds in tobacco through a microorganism combination method. Nicotine in tobacco leach liquor is degraded through nicotine degrading microorganisms, the content of nicotine in the tobacco leach liquor is reduced, the environment of the tobacco leach liquor is improved, and the tolerance of mycobacteria is improved; by adding sterol degrading microorganisms, through the sterol degrading capacity of the microorganisms, the content of sterol in the tobacco leach liquor is reduced. It is proved through experiments that the effect of reducing the sterol compounds in tobacco by means of the microorganism combination method is far better than the effect of reducing tobacco leach liquor sterol through single microorganisms (mycobacteria), the degradation rates of stigmasterol, cholesterol and beta-sitosterol are improved by 79.1%, 47.0% and 65.2% respectively, and the method is a biological harm reduction technology with high targetingproperty.

Description

Technical field [0001] The invention belongs to the field of tobacco biotechnology and tobacco harm reduction, and relates to a method for degrading sterol compounds in tobacco, in particular to a method for degrading sterol compounds in tobacco by a microbial combination method. Background technique [0002] Sterol compounds in tobacco mainly include stigmasterol, β-sitosterol, cholesterol and campesterol. Moldy tobacco also contains ergosterol, and the total content in tobacco is about 0.1-0.3%. Tobacco sterols can form harmful substances of benzo(a)pyrene during pyrolysis, so reducing sterols in tobacco is of great significance for reducing tobacco harm. Sterol is the precursor compound of benzo(a)pyrene in smoke, and it has been confirmed as one of the serious carcinogens. Therefore, the research and development of the degradation pathways and methods of sterol compounds in tobacco has important application value and significance for reducing tobacco harm and improving tobac...

Claims

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Application Information

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IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 张展叶建斌冯颖杰王璐周浩郝辉杨雪鹏毛多斌
Owner CHINA TOBACCO HENAN IND
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