52 results about "Dehydrocholesterols" patented technology

The present invention relates to the field of pharmaceutical chemistry, and in particular to methods of preparing cholesterol,and derivatives and analogs thereof. The cholesterol derivatives include, but not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25- hydroxy7dehydrocholesterol and ergosterol. In the invention, phytosterol can be used as a raw material to prepare the compound shown in the formula I through microbial conversion, and then cholesterol and the derivatives and analogues thereof are prepared.

The invention relates to belongs to 24-dehydrocholesterol reductase coded recombinant adenoviruses specifically expressed in tissue, which belongs to the Alzheimer's disease gene treatment method and the treatment field. More specifically, according to the invention, by aid of a gene adenovirus carrier, the coded gene of 24-dehydrocholesterol reductase (DHCR 24) is introduced in nerve cells, the expression of 24-dehydrocholesterol protein in nerve cells is regulated, and the nerve cells protection effect can be performed. The invention also relates to a construction of defective recombinant adenoviruses for replication comprising nerve specifically expressed DHCR 24 gene and an application of the carriers in DHCR 24 gene in cells and an application of the DHCR 24 protein in nerve cells specific expression. The prepared recombinant adenoviruses are expected to obtain positive curative effect in neurodegenerative diseases such as Alzheimer's disease or other gene treatments which require a DHCR 24 supplementary therapy.

The invention relates to a method for virtually screening cholesterol degrading medicine with 24-dehydrocholesterol reductase (DHCR24) being a target point. The method includes the steps that 1, the molecular structure of DHCR24 is determined; 2, molecular docking software is adopted, the active center of macromolecular docking of the medicine is determined according to a combined pocket which is formed after desmosterol and DHCR24 are docked, and an active pocket is set; 3, a macromolecular ligand databse used for docking is arranged; 4, according to the set active pocket, by the utilization of the molecular docking software, macromolecular ligands in the macromolecular ligand database used for docking and the active pocket are docked in sequence; 5, results which are screened after primary docking are accurately docked, and the candidate medicine having the effect of cholesterol degrading are determined. By the adoption of the method, within short time, the macromolecular candidate medicine which has the effect of competitive restraining of DHCR24 and is developed into the novel cholesterol degrading medicine is obtained, in this way, the research and development efficiency is greatly improved, and the research period of new medicine is shortened.

A health-care fluorescent-lamp powder can prevent osteoporosis. It is a kind of alkali boron phosphate with expression formula ((Mo)1-3. (B203)1-4. (P205)0.1-1:Pb0.005-1. Y0.01-1), M belongs to one or above of calcium, magnesium, strontium and sulfone, and Y is one or above of rare-earth ion lanthanum, samarium and gadolinium. The process is carried out by mixing one or above of calcium carbonate, strontium carbonate and barium carbonate with one or above of magnesium oxide, boric acid, ammonium dibasic phosphate, lead oxide, lanthanum oxide, gadolinium oxide and samarium sesquioxide and solid-phase firing at high-temperature. It can transmit medium-wave ultraviolet with peak value=2970, convert human-body hypodermic 7-deoxycholesterol into vitaminD3, and improve human-body calcium-phosphorus metabolism. It can be used for health-care fluorescent-lamp in family and hospital.

The invention belongs to the technical field of biochemical engineering and enzyme engineering, and in particular relates to a method utilizing the microsomal enzyme of an animal liver to biosynthesize 7-dehydrocholesterol. The method includes the following steps: (1) preparation of microsomal enzyme solution; (2) enzymatic conversion reaction; (3) saponification reaction; and (4) extraction. The method can prepare the 7-dehydrocholesterol which is completely identical with that prepared by chemosynthesis; and meanwhile, the technology is simple and easy, and not only the production cost of vitamin D3 can be reduced, but also the environment pollution can be reduced.

The invention relates to a tobacco 7-dehydrocholesterol reductase gene and application thereof. The nucleotide sequence of the 7-dehydrocholesterol reductase gene is as shown in SEQ ID NO. 1. Based on the important influence of sterol on the safety of tobacco leaves, the tobacco sterol metabolism regulation gene is deeply studied, so that a new tobacco variety is constructed by utilizing genetic engineering, and a good application foundation is laid for improving the tobacco variety. According to the application, preliminary research on specific tobacco sterol 7-dehydrocholesterol reductase shows that the specific tobacco sterol 7-dehydrocholesterol reductase is highly related to the sterol content in tobacco leaves, when the gene is silenced in Bensi tobacco, the stigmasterol content and the campesterol content in the tobacco leaves are reduced, and the total sterol content is reduced by 24.8%. Based on the characteristic, a certain foundation and reference can be provided for tobacco quality regulation and control and new tobacco variety cultivation.

The invention discloses a liquid 25-hydroxyvitamin D calibrator diluent, which comprises the following components: a buffer solution, a protein stabilizer, a preservative and a cyclopentane polyhydrophenanthrene stabilizer. The main innovation of the invention lies in that a) 7-dehydrocholesterol is applied to the 25-hydroxyvitamin D calibrator for the first time for improving the stability of 25-hydroxyvitamin D under a liquid aerobic condition; b) all the components in the calibrator diluent are optimal formulas, so that the stability of the 25-hydroxyvitamin D calibrator is powerfully guaranteed, and the 25-hydroxyvitamin D calibrator can be stably stored at 8 DEG C for 12 months; and c) the liquid 25-hydroxyvitamin D calibrator is applied to detection of 25-hydroxy vitamin D in the serum sample, the calibrator does not need to be redissolved during detection, and the repeatability of a calibration curve which is calibrated for multiple times is superior to that of the calibration curve of the freeze-dried calibrator.

The invention provides clone and expression aiming at 7,8-dehydrogenase gene. The gene encoding 7,8-dehydrogenase is acquired from a constructed drosophila cDNA library, wherein the 7,8-dehydrogenase can transform cholesterol into 7-dehydrocholesterol, thereby providing a new method for synthesizing the 7-dehydrocholesterol by a biological process, acquiring a process route capable of shortening vitamin D3, reducing production cost, and reducing environmental pollution.

The invention discloses saccharomyces cerevisiae engineering bacteria for producing campesterol and a construction method, and belongs to the field of genetic engineering and bioengineering. An expression cassette element of 7-dehydrocholesterol reductase is introduced into a yeast cell body, and is integrated into a yeast genome by utilizing a CRISPR/Cas9 gene operating system, C-22 sterol desaturase is knocked out, competition of an ergosterol branch path is relieved, and yeast synthesis from glucose to campesterol is realized. The method is simple in process, green and environment-friendly, and can be used for producing campesterol through fermentation. The yield of campesterol prepared by adopting the saccharomyces cerevisiae engineering strain BY4742-delta erg5-TEF1p-dhcr7-ADH1t provided BY the invention can reach 253.35mg/L in a shake flask stage, and the horizontal yield of a fermentation tank can reach 916.88mg/L, which is increased by 2.6 times compared with the yield of campesterol in the shake flask stage of the strain.

The invention relates to the technical field of purification of intermediates, in particular to a method for purifying 24-dehydrocholesterol, which comprises the following steps: (1) taking a mixture containing a compound as shown in a formula (I) and a compound as shown in a formula (II) as a raw material to be purified, and obtaining a first purified product in a mode of step (1A) or (1B); (1A) in the presence of a first solvent, contacting the raw material to be purified with MXn to obtain a complex, and then decomplexing in the presence of water and a second solvent; (1B) carrying out column chromatography separation on the raw material to be purified; (2) crystallizing the first purified product by using a third solvent to obtain a second purified product, wherein R in the formula (I) and R in the formula (II) are the same and are H or C1-C4 acyl groups. The method provided by the invention has the advantages of low cost, simple technical process and capability of effectively purifying 24-dehydrocholesterol.

The invention discloses a recombinant yeast engineering strain for producing 7-dehydrocholesterol and application of the recombinant yeast engineering strain, and belongs to the technical field of genetic engineering and biological engineering. According to the invention, endogenous genes ERG5 and ERG6 of saccharomyces cerevisiae are knocked out, delta (24)-sterol reductase from Gallus gallus is expressed, synthesis of 7-dehydrocholesterol is promoted by regulating expression of genes of endogenous related pathways, the yield of 7-dehydrocholesterol after fermentation for 72 hours is 440.92 mg/L, and the yield of 7-dehydrocholesterol after fermentation for 96 hours is 520.03 mg/L, so that a foundation is laid for subsequent biosynthesis of steroid compounds; the method has potential value and significance for the development of synthetic biology.

The invention belongs to the field of gene engineering, and particularly relates to a prawn Y-organ 7,8-dehydrogenase gene and application thereof. The invention provides a gene for coding 7,8-dehydrogenase, which is obtained from a constructed Metapenaeus ensis Y-organ cDNA (complementary deoxyribonucleic acid) library; and the nucleotide sequence of the gene is disclosed as SEQ ID NO.1. The application method comprises the following steps: cloning the 7,8-dehydrogenase coding gene, and connecting with an expression plasmid to construct a recombinant expression plasmid; after linearizing and purifying the recombinant expression plasmid, converting a receptor bacterium, and screening to obtain a gene engineering bacterium; and carrying out induction expression, purification and identification to obtain the 7,8-dehydrogenase of which the amino acid sequence is disclosed as SEQ ID NO.2. The 7,8-dehydrogenase can be used for converting cholesterol into 7-dehydrocholesterol by a one-step enzyme process, thereby enhancing the conversion efficiency, shortening the process route for producing vitamin D3, lowering the production cost and reducing the environmental pollution.

The present invention relates to 7-dehydrocholesterol-hemimethanolate. It has been found that the formation of 7-dehydrocholesterol-hemimethanolate offers an efficient method of increasing the storage stability of 7-dehydrocholesterol, respectively of decreasing the degradation upon storage of 7-dehydrocholesterol.

The invention discloses a food vitamin enhancer and a preparing method thereof. The food vitamin enhancer can be obtained by stirring and then drying ergosterol, cyclohexane, livers, isopropanol, milk, 7-dehydrocholesterol, yolk, a neutralizer, soybean oil, phosphoric acid, yeast, magnesium oxide, chloroform and auxiliaries. The melting point of the product is 100-120 DEG C. The weight loss on drying is 0.1-0.3%, and the product comprises 0.01-0.1% of residue on ignition, 0.001-0.003% of heavy metal and 0.0001-0.0003% of arsenic on the basis of the content of 98-105%.