Method for extracting maize germ oil by adopting ultrasonic-assisted aqueous enzymatic method

A technology of corn germ oil and corn germ powder, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc., to achieve mild reaction conditions, easy operation, and reduce adverse effects

Inactive Publication Date: 2015-07-01
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been rare reports in the literature and patents on the use of ultrasonic pretreatment methods to assist aqueous enzymatic extraction of corn germ oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put the corn germ into a pulverizer to pulverize, pass through a 60-mesh standard sieve to obtain corn germ powder, add a buffer solution with a pH value of 4, and prepare the mass ratio of the corn germ powder to the buffer solution as 1:6. Under the condition of 40 ℃, ultrasonic treatment for 20min, processing power is 400W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 5.5, add 1600U / g cellulase and 1600U / g α-amylase respectively, water bath Stir magnetically at 50°C for 5 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 76.14%.

Embodiment 2

[0026] The corn germ is put into a pulverizer to grind, and the corn germ powder is obtained through a 60-mesh standard sieve, and a buffer solution with a pH value of 3 is added, and the mass ratio of the corn germ powder and the buffer solution is 1:5. Under the condition of 30°C, ultrasonic treatment for 30min, the processing power is 300W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 6.0, add 1200U / g of cellulase and 1200U / g of α-amylase respectively, water bath Stir magnetically at 55°C for 6 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil the water bath for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 72.78%.

Embodiment 3

[0028] The corn germ is put into a pulverizer to grind, and the corn germ powder is obtained through a 60-mesh standard sieve, and a buffer solution with a pH value of 5 is added, and the mass ratio of the corn germ powder and the buffer solution is 1:7. Under the condition of 50 ℃, ultrasonic treatment for 10min, the processing power is 500W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 5.0, add 2000U / g cellulase and 2000U / g α-amylase respectively, water bath Stir magnetically at 45°C for 4 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil the water bath for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 68.99%.

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Abstract

The invention relates to a method for extracting maize germ oil by adopting an ultrasonic-assisted aqueous enzymatic method. The method for extracting the maize germ oil by adopting the ultrasonic-assisted aqueous enzymatic method is characterized by comprising the following steps: smashing maize germs taken as a raw material, sieving, then adding citric acid-sodium citrate buffer solution, and carrying out ultrasonic pretreatment; cooling, regulating pH value, then adding cellulose and alpha-amylase, stirring in constant-temperature water bath, and carrying out enzymatic hydrolysis; after the enzymatic hydrolysis is finished, deactivating enzymes, cooling to room temperature, centrifuging a sample, collecting supernate, demulsifying emulsion, centrifuging secondarily, then separating supernate, and mixing the supernates, so that the maize germ oil is obtained. The method for extracting the maize germ oil by adopting the ultrasonic-assisted aqueous enzymatic method has the advantages of mild conditions, high oil yield, no pollution, easy operation, safety, rapidness and applicability of large-scale production.

Description

technical field [0001] The invention relates to the technical field of vegetable oil extraction, in particular to a method for extracting corn germ oil by ultrasonic-assisted aqueous enzymatic method. Background technique [0002] Corn germ oil is one of the three health oils recommended by the World Health Organization. It is widely consumed as a high-grade edible oil and enjoys the reputation of "healthy oil", "rest assured oil" and "longevity oil". It contains a large amount of unsaturated fatty acids, among which linoleic acid is an essential fatty acid for human body, which is an integral part of human cells. It can be combined with cholesterol in the human body, showing fluidity and normal metabolism. The efficacy of the disease. In addition to unsaturated fatty acids, it is also rich in vitamin E, A, D, phospholipids and amino acids and many other nutrients, and its nutritional value is very rich. Sitosterol in corn germ oil has the effect of lowering cholesterol. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00
CPCC11B1/00C11B1/04C11B3/001
Inventor 韩翠萍王迪刘畅赵月江杨娟李小雅
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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