Method for extracting maize germ oil by adopting ultrasonic-assisted aqueous enzymatic method
A technology of corn germ oil and corn germ powder, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc., to achieve mild reaction conditions, easy operation, and reduce adverse effects
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Embodiment 1
[0024] Put the corn germ into a pulverizer to pulverize, pass through a 60-mesh standard sieve to obtain corn germ powder, add a buffer solution with a pH value of 4, and prepare the mass ratio of the corn germ powder to the buffer solution as 1:6. Under the condition of 40 ℃, ultrasonic treatment for 20min, processing power is 400W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 5.5, add 1600U / g cellulase and 1600U / g α-amylase respectively, water bath Stir magnetically at 50°C for 5 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 76.14%.
Embodiment 2
[0026] The corn germ is put into a pulverizer to grind, and the corn germ powder is obtained through a 60-mesh standard sieve, and a buffer solution with a pH value of 3 is added, and the mass ratio of the corn germ powder and the buffer solution is 1:5. Under the condition of 30°C, ultrasonic treatment for 30min, the processing power is 300W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 6.0, add 1200U / g of cellulase and 1200U / g of α-amylase respectively, water bath Stir magnetically at 55°C for 6 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil the water bath for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 72.78%.
Embodiment 3
[0028] The corn germ is put into a pulverizer to grind, and the corn germ powder is obtained through a 60-mesh standard sieve, and a buffer solution with a pH value of 5 is added, and the mass ratio of the corn germ powder and the buffer solution is 1:7. Under the condition of 50 ℃, ultrasonic treatment for 10min, the processing power is 500W, after ultrasonic treatment, cool to room temperature, adjust the pH value to 5.0, add 2000U / g cellulase and 2000U / g α-amylase respectively, water bath Stir magnetically at 45°C for 4 hours. After the enzymolysis reaction, raise the temperature of the water bath to 100°C and boil the water bath for 10 minutes. After inactivating the enzyme, centrifuge at 5000r / min for 20 minutes, centrifuge twice, and collect the supernatant. The clear oil extraction rate of corn germ oil obtained by this method is 68.99%.
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