Method for extracting Cyperus esculentus oil by aqueous enzymatic extraction-freeze-thaw coupling technology

A technology for extracting oil and water enzymatic method, which is applied in the directions of fat oil/fat production, fat generation, etc., can solve the problems such as no relevant reports on the extraction of sesame oil, low oil extraction rate and low oil extraction rate by water enzymatic method, To achieve good oil quality, no solvent pollution, and improve the effect of oil extraction

Inactive Publication Date: 2012-01-11
XINJIANG UNIVERSITY +1
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  • Abstract
  • Description
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Problems solved by technology

[0017] However, the water enzymatic oil extraction technology has an inevitable disadvantage: the oil extraction rate is low, which will cause waste of raw materials to a certain extent
[0020] However, the oil extraction rate of the hydroenzyme method is low. According to reports, the oil extraction rate of the hydroenzyme method is lowered by nearly 22% than the traditional vegetable oil production method, and the hydroenzyme method-freeze-thaw coupling technology can overcome this shortcoming and improve Therefore, the research significance of the hydroenzymatic method-freeze-thaw coupling technology is very far-reaching. As far as the oily bean is concerned, Yan Han, Qin Ye, etc. have published research on the extraction of oily bean oil by the aqueous enzymatic method, but the water enzyme There is no relevant report on the extraction of oil from Yousha soybean oil by method-freeze-thaw coupling technology

Method used

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  • Method for extracting Cyperus esculentus oil by aqueous enzymatic extraction-freeze-thaw coupling technology
  • Method for extracting Cyperus esculentus oil by aqueous enzymatic extraction-freeze-thaw coupling technology
  • Method for extracting Cyperus esculentus oil by aqueous enzymatic extraction-freeze-thaw coupling technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment one: the method for extracting Yousha bean oil

[0046] See attached figure 1 , using the hydroenzyme method-freezing-thawing coupling technology to extract the method of Yousha soybean oil, the specific extraction steps are as follows:

[0047] (1) Cleaning: Select plump, pest-free oily beans and wash with water to remove surface impurities and dry the surface moisture.

[0048] (2) Pulverization: pulverize evenly with a miniature universal pulverizer.

[0049] (3) Enzyme elimination: take by weighing 500kg oily bean powder, sodium carbonate and sodium bicarbonate by the mixed solution buffer solution (mol / L) of 1: 7 (m / v) solid-liquid ratio, adjust pH value to be 7,50 Soak for 1 hour at ℃, inactivate enzyme at 90℃ for 10 minutes;

[0050] (4) Enzymolysis: Alkaline protease and cellulase composite enzymes are used, and the ratio of protease and cellulase is 2:1 (m / m). The ratio of solid to liquid is 1:6 (m / v), the enzymolysis time is 6 hours, the amount ...

Embodiment 2

[0053] Embodiment two: the mensuration of average oil content

[0054] Put about 3g of finely ground sample into the weighed filter paper bag, seal the mouth of the bag, put it in an oven at 105±2°C to dry for 3 hours, move it to a desiccator to cool to room temperature, and use long tweezers to extract Inject anhydrous diethyl ether 1.67 times the amount of a siphon into the cylinder, so that the sample package is completely submerged in diethyl ether. Connect all parts of the extractor, connect the flow of condensed water, and carry out extraction in a constant temperature water bath. Adjust the water temperature between 70 and 80°C, so that the condensed and dripping ether becomes beads (refluxing more than 7 times / h), Extract until the ether in the extraction cylinder is drip-checked with filter paper until there is no oil stain (about 6 to 12 hours). After the extraction is completed, take out the filter paper bag with long tweezers, and volatilize the ether in a ventila...

Embodiment 3

[0060] Embodiment three: the selection of enzyme kind and the mensuration of enzyme activity

[0061] 1. Determination of Enzyme Activity

[0062] (1) Determination of protease activity

[0063] ① Reagent

[0064] 0.1mol / L sodium carbonate - sodium bicarbonate buffer (pH10);

[0065] 5% trichloroacetic acid;

[0066] 1% casein solution:

[0067] Weigh 5 grams of casein and place it in 500 ml of 0.1 mol / L sodium carbonate-sodium bicarbonate buffer solution (pH10), and heat to dissolve it.

[0068] 0.4mol / L sodium carbonate solution;

[0069] 100μg / ml tyrosine solution;

[0070] Folin reagent:

[0071] Add 100 grams of sodium tungstate (Na2WO4.2H20) and 25 grams of sodium molybdate (Na2MO2.2H20) into a 2000 ml grinding mouth reflux device, add 700 ml of water, 50 ml of 85% phosphoric acid, and 100 ml of concentrated hydrochloric acid, and reflux for 10 hours. Take off the reflux condenser, add 50 grams of lithium sulfate, 50 milliliters of water and a few drops of concen...

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Abstract

The invention discloses a method for extracting Cyperus esculentus oil by the combination of aqueous enzymatic extraction and freeze-thaw, selecting plump Cyperus esculentus without insect diseases, removing impurities on the surface by washing with the use of water, and drying water on the surface; crushing, weighing 500kg of a Cyperus esculentus powder, adding a mixed buffer solution of sodium carbonate and sodium bicarbonate at the solid-liquid ratio of 1:7 (m / v), adjusting pH value to 7, immersing for 1 hour at the temperature of 50 DEG C, killing enzyme for 10 min at the temperature of 90 DEG C; adopting a compound enzyme of alkali protease and cellulase mixed at the mass ratio of 2:1 (m / m) while the solid-liquid ratio is 1:6, the enzymatic hydrolysis time is 6 hours, the enzyme addition is 2.5% and the enzymatic hydrolysis temperature is 70 DEG C; placing the enzymatic hydrolysis solution into an ultralow temperature refrigerator at the temperature of minus 30 DEG C for 30 min after enzymatic hydrolysis, followed by thawing at room temperature; centrifuging for 20 min at the rotating speed of 4000r / min, carrying out secondary centrifugation, and separating crude oil to obtain the product. According to the invention, the oil extraction rate is raised, and good grease quality is also maintained.

Description

field of invention [0001] The invention relates to the technical field of vegetable oil extraction, in particular, the present invention relates to the technical field of oil extraction of oleander and soybean oil. Background technique [0002] As we all know, among the various components of human food, oil has the highest value as a life energy source, with a calorie content of 37.7 kJ / g, about twice that of protein and carbohydrates. The oil industry can be said to be a cause of eternal human needs. my country's developing oil industry has a very broad prospect. It is predicted that China's population will reach 1.5 billion in 2020, if the per capita consumption recommended by the FAO is estimated at 14kg / a. The total consumption of fats and oils is 21Mt, that is, in addition to the need to import 7Mt. The country still produces 14Mt of edible oil, so the national oil processing volume will increase by 72% on the basis of 2000. [0003] Oil sedge, also known as oil sedge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 敬思群王耀祥
Owner XINJIANG UNIVERSITY
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