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Method for extracting rapeseed oil and passivated myrosinase from rapeseeds

A technology of rapeseed oil and rapeseed, which is applied in the field of extracting rapeseed oil and inactivating glucosidase, can solve the problems of high content of glucosinolate and its degradation products in cake, waste of high-quality protein resources, and low extraction rate of free oil. , to achieve the effect of shortening the oil production cycle, eliminating the use of enzymes, and reducing the detoxification process

Active Publication Date: 2016-06-15
JIANGNAN UNIV
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  • Description
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for extracting rapeseed oil and inactivating glucosidase from rapeseed, aiming to solve the problem of high content of glucosinolate and its degradation products, low extraction rate of free oil, and residues caused by traditional techniques. Problems such as high residual oil rate in the phase, waste of high-quality protein resources, large enzyme consumption, high cost or serious environmental pollution

Method used

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  • Method for extracting rapeseed oil and passivated myrosinase from rapeseeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Dried rapeseeds are not pretreated, only crushed, pre-crushed by a traditional Chinese medicine grinder, and then crushed by a three-roller grinder to a volume average particle size of about 45 μm to obtain oil extraction raw materials.

[0039] The oil extraction raw material is carried out at 50°C, according to the material-water ratio of 1:6 (w / v), and the pH of the system is adjusted to 5.0, and 3% pectinase, cellulase, and xylanase are added, and the enzymatic hydrolysis time is 3 hours. After the enzymatic hydrolysis, adjust the pH to 9.0 with 2N sodium hydroxide, raise the temperature to 60°C, and carry out alkaline extraction for 1.5 h. After the alkaline extraction, add 3% alkaline protease and continue enzymatic hydrolysis for 1 hour. Centrifuge at 6000rpm for 10min after the reaction to obtain incompatible four phases—free oil, emulsion, water phase and slag phase, obtain free rapeseed oil with a scoop, and weigh it.

[0040] The extraction rate of free oil ...

Embodiment 2

[0042] Dried rapeseeds are directly subjected to pressurized heat treatment, put the soaked rapeseed 2 into an autoclave for pressurized heat treatment, the pressure is 0.50Mpa, the temperature is 110°C, the time is set to 20min, and the processed rapeseeds are collected 3.

[0043] The steam-exploded rapeseed was dried in a blast drying oven at 50°C; it was pre-crushed by a traditional Chinese medicine grinder, and then crushed by a three-roller grinder until the volume average particle size was about 28 μm to obtain the raw material for oil extraction.

[0044] The oil extraction raw material is carried out at 50°C, according to the material-water ratio of 1:6 (w / v), and the pH of the system is adjusted to 5.0, and 3% pectinase, cellulase, and xylanase are added, and the enzymatic hydrolysis time is 3 hours. After the enzymatic hydrolysis, adjust the pH to 9.5 with 2N sodium hydroxide, raise the temperature to 60°C, and carry out alkaline extraction for 1.5 h. After the alk...

Embodiment 3

[0048] Dried rapeseed 1 was soaked in deionized water for 5 hours according to the material-to-water ratio of 1:2 (w / v), drained and the filtrate was discarded, and soaked rapeseed 2 was collected.

[0049] The soaked rapeseed 2 is subjected to steam explosion treatment, the steam explosion pressure is set at 1.5MPa±0.1MPa, and the pressure holding time is 180s. After the blasting is over, open the steam blaster collection bin to collect the steam-exploded rapeseed 3 .

[0050] The steam-exploded rapeseed 3 was dried in a blast drying oven at 50°C; pre-crushed by a traditional Chinese medicine grinder, and then crushed by a three-roller grinder to a volume average particle size of about 21 μm to obtain oil extraction raw material I.

[0051] The raw material I obtained is at 70°C, according to the ratio of material to liquid 1:6 (w / v), the concentration of ethanol aqueous solution is 50% (v / v), and the pH of the system is adjusted to 9.0 with 2N sodium hydroxide solution to ex...

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Abstract

The invention discloses a method for extracting rapeseed oil and passivated myrosinase from rapeseeds and belongs to the technical field of agricultural resources.The method has the advantages that raw materials are soaked by deionized water, moisture in cells is turned into steam during steam explosion or pressurization heat-moisture treatment, and cell walls are broken to allow the cell structure to be loose and promote the dissolution of grease in the cells; an ethanol solution is used as the oil extraction medium, glucosinolates and most degradation products are allowed to enter a water phase, the glucosinolate and glucosinolate degradation product contents in cake are lowered, and the detoxification procedure of rapeseed cake is reduced; enzyme use is avoided, oil production cycle is shortened, production cost is reduced, oil product quality is optimized, and the problems that the glucosinolate and glucosinolate degradation product contents of the cake obtained by a traditional rapeseed process are high, high-quality protein resources are wasted, a large amount of enzyme needs to be used, cost is high or environment pollution is severe, and the like are solved.

Description

technical field [0001] The invention relates to a method for extracting rapeseed oil and inactivating glucosidase from rapeseed, belonging to the technical field of agricultural resources. Background technique [0002] Rapeseed is one of the main oil crops in my country. The annual output of rapeseed in the country exceeds 14 million tons, and the provinces in the Yangtze River Basin are the main producing areas of rapeseed. Rapeseed contains 40%-45% fat, 20%-25% protein and 25% carbohydrate. Rapeseed oil is a kind of nutrient-rich oil. The content of unsaturated fatty acid accounts for more than 80%, of which oleic acid is more than 40%. It is easy to be absorbed by the human body and has good frying and anti-oxidation properties. Long-term consumption can prevent arteriosclerosis , promote microvascular circulation, prevent colon cancer, reduce the content of serum cholesterol in the human body, reduce blood pressure and lose weight, especially beneficial to obesity, high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/16A23D9/04
CPCA23D9/04C11B1/04C11B1/10C11B3/16
Inventor 杨瑞金任燕勤李鹏飞张益奇张文斌赵伟华霄
Owner JIANGNAN UNIV
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