Microwave-assisted green solvent wet-process oil extraction method for microalgae

A technology of microalgae and wet method, which is applied in the direction of fat oil/fat production and fat generation, which can solve the problems of high cost of solvent and oil separation, harmful solvents and environment, high equipment cost, etc., and achieve high oil yield and oil quality Good, easy-to-operate effect

Active Publication Date: 2013-03-27
ENN SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] The purpose of the present invention is to propose a new method of microalgae wet oil extraction, which solves the need for drying and dehydration in the current oil extraction of microalgae; the process of breaking the wall and extracting oil is carried out step by step, the process is complicated, and the solvent used for extraction is harmful to people and the environment , high equipment cost, high cost of separation of solvent and oil after extraction, etc.

Method used

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  • Microwave-assisted green solvent wet-process oil extraction method for microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Put 40g of Nannochloropsis algae mud (water content 69.2%) obtained from cultivation, put the algae mud into a blue cap reagent bottle, add 140mL of isopropanol, and tighten the bottle cap. Put it in a microwave oven, microwave for 2 minutes, power 250w, solvent ratio 12:1 (mL / g), finish cooling to room temperature, collect the mixed oil phase and cool it to 5 degrees, keep it for 5 hours, separate the phases, and separate the upper phase into isopropyl The alcohol phase was transferred, and the lower oil phase was transferred to a rotary evaporation flask, and the solvent was removed by rotary evaporation to obtain algae hair oil. Add isopropanol to the residual algal mud and repeat microwave extraction, and use the same method to obtain algal hair oil. Finally, a total of 5.01 g of crude oil was obtained, of which the fatty acid content was 60.788%, and the calculated oil extraction rate was 88.1%.

[0048] Evaluation method of oil extraction rate from microalgae

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Embodiment 2

[0059] The Nannochloropsis algae mud obtained from cultivation is 36.64g (water content 69.2%), put the algae mud into a blue cap reagent bottle, add 140mL of isopropanol, and tighten the bottle cap. Put it into a microwave oven, microwave for 2min, the power is 250w, the solvent ratio is 12:1 (mL / g), finish cooling to room temperature, transfer the mixed oil phase to a rotary evaporating flask, and remove the solvent by rotary evaporation. Add isopropanol to the residual algae mud and repeat the microwave extraction three times, and use the same method to separate the algae hair oil. Finally, a total of 11.51 g of crude oil was obtained, of which the fatty acid content was 15.988%, and the calculated oil extraction rate was 86.3%.

Embodiment 3

[0061] Put 70g of Schizochytrium algae mud (water content is 82%) obtained from cultivation, put the algae mud into a reagent bottle with a blue cap, add 140ml of isopropanol, tighten the bottle cap, and put it in a microwave oven. Set the microwave time to 1.5min, the microwave power to 450W, and the solvent ratio to 8:1 (ml / g), finish cooling to room temperature, collect the mixed oil phase and cool to 8 degrees, keep it for 6h, separate the phases, and separate the upper phase The propanol phase was transferred to a rotary evaporation flask, and the solvent was removed by rotary evaporation. Add 140mL isopropanol to the residual algae mud and repeat the microwave extraction twice, and use the same method to separate the algae hair oil. Finally, a total of 7.23 g of crude oil was obtained, of which the fatty acid content was 63.658%, and the calculated oil extraction rate was 92%.

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Abstract

The invention discloses a wet-process oil extraction method for microalgae, comprising the steps of adding a medium-polarity solvent in microalgae mud, and performing microwave heating extraction. Wall breakage and oil extraction for microalgae are performed simultaneously in the method, the process is simple and easy to operate, and the oil of wet microalgae can be rapidly extracted in one step, thus facilitating realizing industrialization; the method is suitable for oil extraction for the algae produced by autotrophic cultivation and heterotrophic cultivation, capable of achieving a high oil extraction rate, and free from the limits of cultivation methods; the obtained oil is good in quality and beneficial to maintaining the high-added-value active ingredients of microalgae; and a slightly-toxic or lowly-toxic organic solvent with an oil-dissolving capacity is used for extraction and harmless to environment, wherein in particular, isopropanol is an extraction solvent for vegetable oil which is recommended by FDA (Food and Drug Administration), and capable of being used for extracting and processing the high-nutrition oil of microalgae.

Description

technical field [0001] The invention relates to the field of separation of plant components, in particular to a microwave-assisted microalgae wet oil extraction method. Background technique [0002] Microwave extraction is a newly developed new technology that uses microwave energy to increase the extraction rate. Its principle is that in the microwave field, the difference in the ability to absorb microwaves makes certain areas of the matrix material or certain components in the extraction system selectively heated, so that the extracted material is separated from the matrix or system and enters the medium. Among extractants with small electric constant and relatively poor microwave absorption ability; microwave extraction has the following advantages: [0003] 1. Traditional thermal extraction is carried out from outside to inside by means of heat conduction and heat radiation, while microwave-assisted extraction is heated inside and outside at the same time. There is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/00
Inventor 杨巧利朱振旗徐春保杜彦山
Owner ENN SCI & TECH DEV
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