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Demulsification treatment method for aqueous enzymatic method extracted tea seed oil

A technology of tea seed oil and processing method, which is applied in the direction of fat oil/fat production and fat generation, so as to achieve the effect of improving economic benefits and improving oil extraction rate

Inactive Publication Date: 2016-06-01
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nowadays, many researchers have carried out in-depth research on enzymatic demulsification. There have been different degrees of progress in the enzymatic demulsification of peanut oil, soybean oil, and wheat germ oil. In the water enzymatic extraction of tea seed oil Research reports have not yet covered the exploration of demulsification, which is a topic worth studying

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  • Demulsification treatment method for aqueous enzymatic method extracted tea seed oil

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Embodiment 1

[0026] The demulsification treatment method of extracting tea seed oil by aqueous enzymatic method of the present invention is realized through the following steps:

[0027] S1. Collect emulsion: use neutral protease, react in a magnetic stirrer at 53°C for 3.2h at a material-to-liquid ratio of 1:5.19, after the reaction is completed, inactivate the enzyme at 100°C for 10min, put it into a centrifuge tube after cooling, and heat at 4000r Centrifuge at 1 / min for 20 minutes, suck out the supernatant oil with a pipette gun, and collect the emulsified layer;

[0028] S2, using alkaline protease to demulsify the emulsion;

[0029] S2.1. Preheating the instrument: turn on the magnetic stirrer and set the temperature to 53°C;

[0030] S2.2, adjust the pH value: adjust the pH value of the emulsion to 8.5 with 1mol / L NaOH;

[0031] S2.3. Reaction: Take the enzyme with a pipette, add Genencor food-grade alkaline protease Protex6L (liquid) to the emulsion after adjusting the pH value, ...

Embodiment 2

[0039] The difference between this embodiment and Embodiment 1 lies in step S2.3.

[0040] S2.3. Reaction: Add food-grade 2709 alkaline protease to the emulsion after adjusting the pH value. The dosage of the enzyme is 4000U / g emulsion, and put it into the rotor. React in the container for 15 minutes.

[0041] The demulsification rate of this embodiment is 91.9%.

Embodiment 3

[0043] The difference between this embodiment and Embodiment 1 lies in steps S2.2 and S2.3.

[0044] S2.2, adjust the pH value: adjust the pH value of the emulsion to 9 with 1mol / L NaOH;

[0045] S2.3. Reaction: Add Suleibao alkaline protease to the emulsion after adjusting the pH value. The dosage of the enzyme is 7000U / g emulsion, and put it into the rotor. React in the container for 15 minutes.

[0046] The demulsification rate of this embodiment is 86.8%.

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Abstract

The invention discloses a demulsification treatment method for aqueous enzymatic method extracted tea seed oil. According to the demulsification treatment method, emulsion generated when tea seed oil is extracted by using an aqueous enzymatic method is collected in a centrifugal mode, and alkaline protease is adopted to break stability of the emulsion, so that clear oil can be extracted. The oil extraction rate of tea seed oil can be increased, and the economic benefits can be increased. By the adoption of the demulsification treatment method disclosed by the invention and under appropriate reaction conditions, the demulsification rate can be up to 92.1%, and the technical difficulty of demulsification can be solved before industrialized application of tea seeds.

Description

technical field [0001] The invention relates to extracting tea seed oil by an aqueous enzymatic method, in particular to a demulsification treatment method for extracting tea seed oil by an aqueous enzymatic method. Background technique [0002] Aqueous enzymatic oil extraction technology is a recent oil extraction technology that has attracted more attention at home and abroad. Compared with other oil extraction methods, aqueous enzymatic method has the advantages of safety, low energy consumption, good product quality, and convenient operation. However, a large amount of emulsion produced during the oil extraction process seriously affects the separation of free oil, resulting in a low oil extraction rate and a decline in economic benefits. Demulsification of emulsion has become a technical problem that must be solved before the industrial application of this process. Nowadays, many researchers have carried out in-depth research on enzymatic demulsification. There have be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 王晓琴曾子菲
Owner HUAQIAO UNIVERSITY
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