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Method for extracting schizochytrium aggregatum oil

A technology for Schizochytrium and oil, which is applied in the field of oil extraction from Schizochytrium, can solve problems such as no relevant reports, and achieve the effects of simple process, less pollution and safe operation.

Inactive Publication Date: 2011-09-28
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Many scholars at home and abroad have widely applied this process to the oil extraction of various oilseeds in the laboratory, but there is no related report on the extraction of oil from Schizochytrium sp.

Method used

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Examples

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preparation example Construction

[0035] The preparation method of the fermentation medium: 120g of glucose, 20g of yeast extract, 15g of peptone, 3g of potassium dihydrogen phosphate, 2g of magnesium sulfate, 10ml of trace element compound solution, 5ml of vitamin compound solution, and 0.5 × seawater (diluted with fresh water 1 times seawater) to 1L. Seawater: taken from the waters of Xiamen.

[0036] Preparation method of trace element compound solution: Na 2· EDTA 6.0g, FeCl 3 ·6H 2 O 0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 6H2O 0.026g, NiSO 4 ·6H 2 O 0.052g, CuSO 4 ·5H 2 O0.002g, NaMoO 4 2H 2 O 0.005g, make up 1L with distilled water.

[0037] The preparation method of vitamin complex solution: vitamin B1 (thiamin) 100mg, vitamin H (biotin) 0.5mg, vitamin B12 (cyanocobalamin) 0.5mg, distilled water to supplement 1L.

Embodiment 1

[0038] Embodiment 1, extract Schizochytrium oil

[0039] Weigh the Schizochytrium mud with a wet weight of 100g (moisture content 33%, oil content about 18g), add 0.05mol / L sodium citrate buffer solution with a pH value of 4.8, 150ml, homogeneous, high temperature (121°C) Sterilize for 15 minutes. Adjust the pH value of the homogeneous solution to 9.5 with NaOH solution, and carry out alkaline extraction at a constant speed of 2 hours at a temperature of 60°C. After the alkali extraction, adjust the pH value of the system to 7.0 and the temperature at 55°C, add neutral protease with a dry weight of 2.5% of the dry weight of the Schizochytrium algae mud, and enzymolyze it at a constant speed for 2 hours; Weight 7.5% alkaline protease, enzymolysis at a uniform speed for 6 hours, after cooling, centrifuge at 17696g for 20mim, centrifuge to obtain free oil, emulsion, hydrolyzate and slag, extract the emulsion with 1 / 3 volume of petroleum ether, let it stand for stratification, T...

Embodiment 2

[0040] Embodiment 2, extract Schizochytrium oil

[0041] Weigh the Schizochytrium mud with a wet weight of 100g (33% moisture content, about 18g fat content), add 150ml of sodium citrate buffer solution with a pH of 4.8 at 0.05mol / L, homogenize, and extinguish at high temperature (121°C). Bacteria treatment for 15 minutes. Use NaOH solution to adjust the pH value of the homogeneous solution to 9.5, the temperature is 60°C, and carry out alkaline extraction for 1 hour with constant stirring. After the alkali extraction, adjust the pH value of the system to 7.0 and the temperature at 55°C, add neutral protease with a dry weight of 2.5% of the dry weight of the Schizochytrium algae mud, and enzymolyze it at a constant speed for 2 hours; 5% alkaline protease, hydrolyzed at a constant speed for 6 hours, after cooling, centrifuged at 17696g for 20mim, centrifuged to obtain free oil, emulsion, hydrolyzate and slag, the emulsion was extracted with 1 / 3 volume of petroleum ether, and a...

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Abstract

The invention discloses a method for extracting schizochytrium aggregatum oil. The method comprises the following steps of: taking schizochytrium aggregatum mud as a raw material, homogenizing, carrying out alkali extraction, centrifuging and extracting with an organic solvent to obtain the schizochytrium aggregatum oil. The method specifically comprises the steps of: diluting the raw material byutilizing a sodium citrate buffering solution, homogenizing and then carrying out high-temperature treatment, then carrying out alkali extraction, carrying out enzymolysis on the raw material subjected to alkali extraction by adopting neutral protease and alkali protease in sequence, centrifuging and then separating clear oil, and extracting missible oil by adopting the organic solvent, and therefore the oil extraction rate is greatly improved and reaches up to more than 90 percent. The method disclosed by the invention has the advantages of simple process, safety in operation and less pollutions and is suitable for large-scale production; cells can be completely crushed by utilizing the homogenizing treatment; various enzymes in the cells can be prevented from degrading oil by adopting the high-temperature treatment; and the treatment on the missible oil is added, and the oil extraction rate is increased by extraction with the organic solvent, standing delamination and other manners.

Description

technical field [0001] The invention relates to a method for extracting Schizochytrium oil. Background technique [0002] Microalgae have the characteristics of short growth cycle, fast reproduction speed, strong plasticity, and simple requirements for nutritional elements, and some of them can achieve high-density fermentation culture through bioengineering methods and control of culture conditions. It has been found that a variety of microalgae are rich in polyunsaturated fatty acids (PUFAs), such as Schizochytrium and Cryptodinophyllum. Microalgae are the initial producers of polyunsaturated fatty acids in the marine food chain. Some algae cells contain high levels of polyunsaturated fatty acids DHA and EPA, and their relative content is as high as 5% to 6% of the dry weight of the cells. The types of fatty acids are relatively simple, and it is relatively easy to separate and purify a single component. Therefore, the use of marine microalgae to produce polyunsaturated f...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B1/00C11B1/04
Inventor 林祥志荣辉
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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