Method for extracting schizochytrium aggregatum oil

Abstract

The invention discloses a method for extracting schizochytrium aggregatum oil. The method comprises the following steps of: taking schizochytrium aggregatum mud as a raw material, homogenizing, carrying out alkali extraction, centrifuging and extracting with an organic solvent to obtain the schizochytrium aggregatum oil. The method specifically comprises the steps of: diluting the raw material byutilizing a sodium citrate buffering solution, homogenizing and then carrying out high-temperature treatment, then carrying out alkali extraction, carrying out enzymolysis on the raw material subjected to alkali extraction by adopting neutral protease and alkali protease in sequence, centrifuging and then separating clear oil, and extracting missible oil by adopting the organic solvent, and therefore the oil extraction rate is greatly improved and reaches up to more than 90 percent. The method disclosed by the invention has the advantages of simple process, safety in operation and less pollutions and is suitable for large-scale production; cells can be completely crushed by utilizing the homogenizing treatment; various enzymes in the cells can be prevented from degrading oil by adopting the high-temperature treatment; and the treatment on the missible oil is added, and the oil extraction rate is increased by extraction with the organic solvent, standing delamination and other manners.

Description

technical field

[0001] The invention relates to a method for extracting Schizochytrium oil. Background technique

[0002] Microalgae have the characteristics of short growth cycle, fast reproduction speed, strong plasticity, and simple requirements for nutritional elements, and some of them can achieve high-density fermentation culture through bioengineering methods and control of culture conditions. It has been found that a variety of microalgae are rich in polyunsaturated fatty acids (PUFAs), such as Schizochytrium and Cryptodinophyllum. Microalgae are the initial producers of polyunsaturated fatty acids in the marine food chain. Some algae cells contain high levels of polyunsaturated fatty acids DHA and EPA, and their relative content is as high as 5% to 6% of the dry weight of the cells. The types of fatty acids are relatively simple, and it is relatively easy to separate and purify a single component. Therefore, the use of marine microalgae to produce polyunsaturated f...