Calcium alginate-sodium carboxymethyl cellulose co-immobilized white rot fungus and its extracellular enzyme biological microcapsules and its preparation method

A technology of sodium carboxymethyl cellulose and calcium alginate, which is applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of affecting enzyme production, white rot bacteria limitation, and increasing application costs, etc. problems, to achieve the effect of improving degradation efficiency, reducing production costs, and reducing start-up time

Active Publication Date: 2018-10-26
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, direct use of white rot fungi in wastewater treatment has some unavoidable disadvantages
First of all, white rot fungi are filamentous fungi with poor shear resistance. If the shear force in the reactor is too large, it will destroy its mycelium structure and affect its enzyme production; Growth and Degradability
In addition, the growth and enzyme production of white rot fungi are greatly affected by the water quality environment, which means that in practical applications, the components of wastewater must be artificially controlled, which limits the scope of its application and increases the application cost.
[0004] Simple immobilization of white rot fungi can effectively improve the adaptability of white rot fungi to the environment, but the enzyme production of white rot fungi is still limited by their own metabolism
A simple immobilized lignin-degrading enzyme system can get rid of the metabolic influence of white rot fungi and improve the treatment efficiency of wastewater. However, except for laccase, the other two enzymes in the lignin-degrading enzyme system use hydrogen peroxide as a direct substrate to start reaction, and the amount of hydrogen peroxide is difficult to control in actual operation, which limits the application of manganese peroxidase and lignin peroxidase

Method used

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  • Calcium alginate-sodium carboxymethyl cellulose co-immobilized white rot fungus and its extracellular enzyme biological microcapsules and its preparation method
  • Calcium alginate-sodium carboxymethyl cellulose co-immobilized white rot fungus and its extracellular enzyme biological microcapsules and its preparation method

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Experimental program
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Embodiment 1

[0048] select Phanerochaete chrysosporium (purchased from Guangdong Microorganism Culture Collection Center, the strain preservation number is GIM3.383) is a white rot fungus strain.

[0049] 1) Preparation of white rot fungus extracellular enzyme cross-linked aggregates: white rot fungi Phanerochaete chrysosporium Inoculate in a liquid medium, culture at a constant temperature of 30°C for 10 days, collect mycelium by filtration, set aside for later use, dialyze the filtrate, and concentrate to obtain a crude enzyme solution; add saturated ammonium sulfate solution and phosphate buffer solution to the crude enzyme solution ( 0.2M, pH7.4), the volume ratio of crude enzyme solution, saturated ammonium sulfate solution and phosphate buffer solution was 1:6:1, and the reaction solution was obtained by aggregating for 15 minutes under stirring with a magnetic stirrer; Dialdehyde, so that the final volume concentration of glutaraldehyde is 0.25%, constant temperature oscillation ...

Embodiment 2

[0056] select as Trametes versicolor (purchased from China Industrial Microorganism Culture Collection Center, the strain collection number is CICC 50001) white rot fungus strains.

[0057] 1) Preparation of white rot fungus extracellular enzyme cross-linked aggregates: white rot fungi Trametes versicolor Inoculate in a liquid medium, culture at a constant temperature of 30°C for 10 days, collect mycelium by filtration, set aside for later use, dialyze the filtrate, and concentrate to obtain a crude enzyme solution; add saturated ammonium sulfate solution and phosphate buffer solution to the crude enzyme solution ( 0.2M, pH7.4), the volume ratio of crude enzyme solution, saturated ammonium sulfate solution and phosphate buffer solution is 1:9, agglomerate for 15min under stirring with a magnetic stirrer to obtain a reaction solution; add 25% glutaraldehyde dropwise to the reaction solution , so that the final volume concentration of glutaraldehyde is 0.3%, crosslinking at c...

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Abstract

The invention relates to a calcium alginate-sodium carboxymethyl cellulose co-immobilized Phanerochaete chrysosporium strain and a biological microcapsule of exoenzyme thereof, and a preparation method of the strain. The biological microcapsule comprises a shell and a shell inside core, wherein the shell material is a calcium alginate gel, and a Phanerochaete chrysosporium exoenzyme crosslinking aggregate is embedded inside the calcium alginate gel; and the shell inside core is a calcium chloride-sodium carboxymethyl cellulose water solution, and Phanerochaete chrysosporium cells and an extracellular crosslinking enzyme aggregate are dispersed inside the calcium chloride-sodium carboxymethyl cellulose water solution. In the biological microcapsule preparation process, the Phanerochaete chrysosporium exoenzyme is directly prepared into the crosslinking enzyme aggregate, thereby omitting the step of purification of the lignin degrading enzyme, effectively lowering the production cost and enhancing the degradation efficiency since the crosslinking enzyme aggregate and Phanerochaete chrysosporium form a close relation.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms and environmental engineering, and relates to a method for co-immobilization of white rot fungi, in particular to a biological microbiological method for co-immobilization of white rot fungi and their extracellular enzymes by calcium alginate-sodium carboxymethyl cellulose Capsules and methods for their preparation. Background technique [0002] White rot fungi can degrade xenobiotics under aerobic conditions, and have broad application prospects in wastewater treatment and environmental restoration. The degradation of xenobiotics by white rot fungi begins in the secondary metabolism stage, mainly relying on the lignin-degrading enzymes secreted by them. The extracellular enzyme system mainly includes lignin peroxidase, manganese peroxidase and laccase, which can achieve indirect degradation of xenobiotics by initiating a series of free radical reactions. [0003] However, the dir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C02F3/34C12R1/645
Inventor 李彦春王智郑力文祝德义徐旭东刘洁
Owner 山东济清科技服务有限公司
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