Calcium alginate-sodium carboxymethyl cellulose co-immobilized white rot fungus and its extracellular enzyme biological microcapsules and its preparation method
A technology of sodium carboxymethyl cellulose and calcium alginate, which is applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of affecting enzyme production, white rot bacteria limitation, and increasing application costs, etc. problems, to achieve the effect of improving degradation efficiency, reducing production costs, and reducing start-up time
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Examples
Embodiment 1
[0048] select Phanerochaete chrysosporium (purchased from Guangdong Microorganism Culture Collection Center, the strain preservation number is GIM3.383) is a white rot fungus strain.
[0049] 1) Preparation of white rot fungus extracellular enzyme cross-linked aggregates: white rot fungi Phanerochaete chrysosporium Inoculate in a liquid medium, culture at a constant temperature of 30°C for 10 days, collect mycelium by filtration, set aside for later use, dialyze the filtrate, and concentrate to obtain a crude enzyme solution; add saturated ammonium sulfate solution and phosphate buffer solution to the crude enzyme solution ( 0.2M, pH7.4), the volume ratio of crude enzyme solution, saturated ammonium sulfate solution and phosphate buffer solution was 1:6:1, and the reaction solution was obtained by aggregating for 15 minutes under stirring with a magnetic stirrer; Dialdehyde, so that the final volume concentration of glutaraldehyde is 0.25%, constant temperature oscillation ...
Embodiment 2
[0056] select as Trametes versicolor (purchased from China Industrial Microorganism Culture Collection Center, the strain collection number is CICC 50001) white rot fungus strains.
[0057] 1) Preparation of white rot fungus extracellular enzyme cross-linked aggregates: white rot fungi Trametes versicolor Inoculate in a liquid medium, culture at a constant temperature of 30°C for 10 days, collect mycelium by filtration, set aside for later use, dialyze the filtrate, and concentrate to obtain a crude enzyme solution; add saturated ammonium sulfate solution and phosphate buffer solution to the crude enzyme solution ( 0.2M, pH7.4), the volume ratio of crude enzyme solution, saturated ammonium sulfate solution and phosphate buffer solution is 1:9, agglomerate for 15min under stirring with a magnetic stirrer to obtain a reaction solution; add 25% glutaraldehyde dropwise to the reaction solution , so that the final volume concentration of glutaraldehyde is 0.3%, crosslinking at c...
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Abstract
Description
Claims
Application Information
- IPC
- C12N11/14; C12N11/10; C02F3/34; C12R1/645
- Inventors
- 李彦春; 王智