Asexual regeneration purity preservation method for hybrid rice of dual-purpose genie sterile line

Abstract

The invention belongs to a method of new plants or acquiring of the new plants, relates to the technical field of plant regeneration of a tissue culture technology, and discloses an asexual regeneration purity preservation method for hybrid rice of the dual-purpose genie sterile line. According to the asexual regeneration purity preservation method for hybrid rice of the dual-purpose genie sterileline, the dual-purpose genie sterile line with good comprehensive characters and good combining ability is selected, an intelligent temperature control constant temperature cold water pond is utilized for identifying the low sterility critical temperature of the dual-purpose genie sterile line, selection pressure at the temperature of 23 DEG C is set, microscopic examination and bagging evaluation are utilized, and the dual-purpose genie sterile line with the sterility critical temperature less than or equal to 23 DEG C is screened. The dual-purpose genie sterile line is subjected to vegetative propagation though a regeneration rice technology, the purity of the sterile line and the stability of the sterility critical temperature can be kept, original states of all characters of parents are kept, the purity of the dual-purpose genie sterile line is ensured, and especially, the low sterility critical temperature of the sterile line reaches 100%.

Description

technical field [0001] The invention belongs to the field of new plants or methods for obtaining new plants; the technical field of plant regeneration through tissue culture technology, and in particular relates to a method for asexual regeneration and purity preservation of dual-purpose male sterile lines of hybrid rice. Background technique [0002] At present, the commonly used prior art in the industry is as follows: the degradation of the seed purity of the dual-purpose nuclear sterile line and the temperature drift of the sterility starting point are the key problems affecting the seed production of the dual-purpose nuclear hybrid rice. The sexual reproduction of the existing dual-purpose GMS lines is through continuous multigenerational reproduction. Under the segregation and selection of traits, the rate of hybrid plants in the sterile lines increases, and some traits change, especially the temperature at the starting point of fertility transformation drifts, resultin...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: the purity of the sterile lines in the prior art is low, the temperature of the starting point of sterility caused by the drift of fertility increases, and the risk of seed production safety increases

Images