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Solid medium pollen and preparation method and use thereof

A solid medium, pollen technology, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as no solution, achieve good economic and social benefits, reduce production costs, and save manpower and material resources. Effect

Inactive Publication Date: 2011-11-16
YUXI ZHONGYAN TOBACCO SEED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no good solution in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The selected demonstration field is located in the seed production base of Yuxi China Tobacco Seed Co., Ltd. in Xishuangbanna, Yunnan. Tobacco seedlings are raised by floating seedlings, which are transplanted into the field after seedlings are formed. The field planting density is: row spacing 1.2m, plant spacing 0.5m.

[0017] In the seed production base, a field of 25 acres of land was implemented. Among them, 5 acres are used as the male parent of MS Yunyan 87, and the remaining 20 acres are used to plant the female parent of MS Yunyan 87. The anthers of the male parent of MS Yunyan 87 were collected in the field and dried to disperse the pollen. The pollen was sifted out with a 50-mesh sieve, and the pollen was collected and sealed and stored at 4°C for use. The starch and the pure pollen of MS Yunyan 87 male parent are evenly mixed in a volume ratio of 1:2 to prepare the required solid medium pollen.

Embodiment 2

[0019] In the seed production base, a field of 25 acres of land was implemented. Among them, 5 acres are used as the male parent of MSK326, and the remaining 20 acres are used as the female parent of MSK326. The anthers of MSK326 male parent were collected in the field and dried to disperse the pollen. The pollen was sifted out with a 50-mesh sieve, and the pollen was collected and sealed and stored at 4°C for later use. The starch and the pure pollen of the MSK326 male parent are mixed uniformly in a volume ratio of 1:2 to prepare the required solid medium pollen.

Embodiment 3

[0021] In the seed production base, a field of 25 acres of land was implemented. Among them, 5 acres are used as the male parent of MS Yunyan 85, and the remaining 20 acres are used for planting the female parent of MS Yunyan 85. The anthers of the male parent of MS Yunyan 87 were collected in the field and dried to disperse the pollen. The pollen was sifted out with a 50-mesh sieve, and the pollen was collected and sealed and stored at 4°C for later use. The starch and the pure pollen of MS Yunyan 85 male parent are mixed uniformly in a volume ratio of 1:2 to prepare the required solid medium pollen.

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PUM

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Abstract

The present invention discloses a solid media pollen, a preparation method and the application thereof. Tobacco pollen parent anther is collected and aired to loose pollen; the pollen is sieved by a 50-mesh sieve; starch or flour is mixed with the collected pure tobacco pollen evenly according to the volume ratio of 1:2-4; and the required solid media pollen is obtained. The solid media pollen isdipped by a cotton swab or a brush pen and is smeared on a female parent tobacco flower stigma for artificial pollination. The use of the solid media pollen improves the effective utilization rate ofthe tobacco pollen, reduces the usage amount of the pure pollen, saves the manpower and material resources used for collecting the pollen and reduces the planting area of tobacco pollen parent and the hybrid seed production cost. The popularization and the application of the technology have good economic benefit and social benefit.

Description

Technical field [0001] The invention belongs to the technical field of artificial pollination, and relates to artificial auxiliary pollination of tobacco hybrid seed production, in particular to a solid medium pollen. At the same time, the invention also relates to the preparation method and application of the solid medium pollen. Background technique [0002] Tobacco (Nicotiana tabacum L.) is an important cash crop and an important source of my country's fiscal revenue. With the development of tobacco agriculture in the world, the popularization and application of tobacco male sterile lines has gradually become one of the main development directions of tobacco leaf production in my country. At present, the technology and production mode of tobacco male sterile lines have been widely promoted and applied in tobacco agricultural production. The planting area of ​​tobacco male sterile lines is expanding year by year, and the amount of seeds used is also increasing year by year. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 马文广郑昀晔李永平陈云松宋碧清索文龙牛永志薛大章
Owner YUXI ZHONGYAN TOBACCO SEED
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