The invention relates to a Delftia sp. For regulating and controlling quorum sensing quenching and a separation method and application thereof, the Delftia sp. For regulating and controlling quorum sensing quenching is named as Delftia sp. JL5 strain, the preservation unit is China Center for Type Culture Collection, the preservation time is January 21, 2021, the preservation number is CCTCCM 2021139, and the address is Wuhan University, Wuhan, Hubei Province. The Delftiasp. is a bacterium for producing acylase, the enzyme expression quantity is very high, compared with rhodococcus BH4 which is most widely applied at present, the Delftia shows higher N-acyl homoserine lactone (AHL) degradation capacity, and when the Delftia is added into a membrane bioreactor (MBR) through the efficient AHL degradation capacity of the Delftia sp., a biological membrane can be inhibited from being attached to the membrane component to prevent and control membrane pollution and blockage, the water outlet efficiency is improved, and the operation cost is reduced.